Abstract:When saturated with ATP, sea cucumber myosin hydrolyzes ATP about 15 times more slowly than rabbit myosin, as expected from the slower contraction time of the muscle. The Arrhenius plots coincide at low temperatures, but only the rabbit plot changes slope at higher temperatures. This unexpected change in slope apparently results from the use of Verona1 buffer. At high substrate concentrations in Verona1 buffer, the enzymatic activities of the two proteins depend identically on calcium ion and on pH. The two my… Show more
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