Hydrophobicity of polymer based anion-exchange columns for ion chromatography

Nesterenko, Pavel N.; Nesterenko, Ekaterina P.

doi:10.1016/j.heliyon.2021.e07290

Cited by 27 publications

(4 citation statements)

References 69 publications

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“…Our study completes the picture by showing that a high level of hydrophobicity delays elution and broadens peaks as can be seen in 6- and 8-drug species, disrupting ion exchange impactfully. On the contrary, in iCIEF, charge variants are separated in soluble matrix driven by absolute isoelectric point; therefore, undesired interaction with stationary phase as in CEX is eliminated. ,, Despite the well-recognized success of IEX on mAbs, its complex separation of ADCs could lead to misleading results. Our findings emphasize the importance of method selection for ADCs during control strategy establishment.…”

Section: Resultsmentioning

confidence: 99%

In-Depth Structure and Function Characterization of Heterogeneous Interchain Cysteine-Conjugated Antibody–Drug Conjugates

Chen,

Qiu,

Shi

et al. 2023

ACS Pharmacol. Transl. Sci.

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Antibody−drug conjugates (ADCs), integrating high specificity of antigen-targeting antibodies and high potency of cellkilling chemical drugs, have become one of the most rapidly expanding therapeutic biologics in oncology. Although ADCs were widely studied from multiple aspects, overall structural elucidation with comprehensive understanding of variants is scarcely reported. Here, for the first time, we present a holistic and in-depth characterization of an interchain cysteine-conjugated ADC, focusing on conjugation and charge heterogeneity, and in vitro biological activities. Conjugation mapping utilized a bottom-up approach, unraveled positional isomer composition, provided insights into the conjugation process, and elucidated how conjugation affects the physicochemical and biological properties of an ADC. Charge profiling combined bottom-up and top-down approaches to interrogate the origin of charge heterogeneity, its impact on function, and best practice for characterization. Specifically, we pioneered the utilization of capillary isoelectric focusing-mass spectrometry to decode not only critical post-translational modifications but also drug load and positional isomer distribution. The study design provides general guidance for in-depth characterization of ADCs, and the analytical findings in turn benefit the discovery and development of future ADCs.

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Section: Resultsmentioning

confidence: 99%

In-Depth Structure and Function Characterization of Heterogeneous Interchain Cysteine-Conjugated Antibody–Drug Conjugates

Chen,

Qiu,

Shi

et al. 2023

ACS Pharmacol. Transl. Sci.

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“…While hydrophobic interaction resulting from either the polymer matrix or the exchange groups even or the linker, also plays an important role in the separation selectivity of the anion exchanger. About this, a more recent article offered a detailed summarization [22]. The use of hydrolyzed GMA-DVB particles to prepare GH-MAPTAC is aimed to reduce the possible hydrophobic interaction from the matrix, which is unwanted towards polarizable anions such as nitrate or bromide.…”

Section: F G U R Ementioning

confidence: 99%

A strong anion exchanger of poly(glycidyl methacrylate‐divinylbenzene) substrate functionalized with cationic quaternary ammonium monomer

Chen

Zhang

et al. 2022

J of Separation Science

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A strong anion exchanger applied for ion chromatography is described by grafting methacrylamido propyl trimethyl ammonium chloride onto hydrolyzed poly(glycidyl methacrylate-divinylbenzene) substrate. The anion exchanger has been in detail characterized and the final capacity of the anion exchanger can be readily manipulated by different monomer amounts. It exhibited obvious temperature-sensitive properties and large tolerance to the samples with high ionic strength. The obtained anion exchanger demonstrated effective separation ability towards the common seven inorganic anions within 12 min and high separation efficiency, for example, 58 000/m of theoretical plate count for chloride.

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“…As the separation mechanisms of HILIC or AEX are strongly associated with polar interactions (polarity, hydrogen bonding, polarizability, etc. ) , or electrostatic and hydrophobic interactions, , respectively, the currently understood separation mechanism of unified-HILIC/AEX is expected to be a combined or individual effect of these physicochemical properties. Thus, RT prediction in unified-HILIC/AEX is more crucial than that in conventional RP-LC and HILIC, and it is essential to improve current prediction methods.…”

Section: Introductionmentioning

confidence: 99%

Predicting Retention Time in Unified-Hydrophilic-Interaction/Anion-Exchange Liquid Chromatography High-Resolution Tandem Mass Spectrometry (Unified-HILIC/AEX/HRMS/MS) for Comprehensive Structural Annotation of Polar Metabolome

Torigoe,

Takahashi,

Heravizadeh

et al. 2024

Anal. Chem.

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The accuracy of the structural annotation of unidentified peaks obtained in metabolomic analysis using liquid chromatography/tandem mass spectrometry (LC/MS/MS) can be enhanced using retention time (RT) information as well as precursor and product ions. Unified-hydrophilic-interaction/anionexchange liquid chromatography high-resolution tandem mass spectrometry (unified-HILIC/AEX/HRMS/MS) has been recently developed as an innovative method ideal for nontargeted polar metabolomics. However, the RT prediction for unified-HILIC/ AEX has not been developed because of the complex separation mechanism characterized by the continuous transition of the separation modes from HILIC to AEX. In this study, we propose an RT prediction model of unified-HILIC/AEX/HRMS/MS, which enables the comprehensive structural annotation of polar metabolites. With training data for 203 polar metabolites, we ranked the feature importance using a random forest among 12,420 molecular descriptors (MDs) and constructed an RT prediction model with 26 selected MDs. The accuracy of the RT model was evaluated using test data for 51 polar metabolites, and 86.3% of the ΔRTs (difference between measured and predicted RTs) were within ±1.50 min, with a mean absolute error of 0.80 min, indicating high RT prediction accuracy. Nontargeted metabolomic data from the NIST SRM 1950−Metabolites in frozen human plasma were analyzed using the developed RT model and in silico MS/MS prediction, resulting in a successful structural estimation of 216 polar metabolites, in addition to the 62 identified based on standards. The proposed model can help accelerate the structural annotation of unknown hydrophilic metabolites, which is a key issue in metabolomic research.

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Hydrophobicity of polymer based anion-exchange columns for ion chromatography

Cited by 27 publications

References 69 publications

In-Depth Structure and Function Characterization of Heterogeneous Interchain Cysteine-Conjugated Antibody–Drug Conjugates

In-Depth Structure and Function Characterization of Heterogeneous Interchain Cysteine-Conjugated Antibody–Drug Conjugates

A strong anion exchanger of poly(glycidyl methacrylate‐divinylbenzene) substrate functionalized with cationic quaternary ammonium monomer

Predicting Retention Time in Unified-Hydrophilic-Interaction/Anion-Exchange Liquid Chromatography High-Resolution Tandem Mass Spectrometry (Unified-HILIC/AEX/HRMS/MS) for Comprehensive Structural Annotation of Polar Metabolome

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