Hydrophobically modified carbon dots as a multifunctional platform for serum-resistant gene delivery and cell imaging

Abstract

Hydrophobically modified carbon dots were prepared and their structure-activity relationship as non-viral gene vectors was investigated.

Carbon dot composites for bioapplications: a review

“…, covalent interaction) of the two. 306–308 Among various CD composites, PEI–CD composites have been paid much attention due to their synthetic maneuverability and high DNA-binding ability. 309–311 For instance, both Dou and Liu's groups have reported PEI functionalized CDs applied for DNA delivery.…”

“…For instance, in Yan and co-workers’ study, glyoxylic acid-modified CDs (GA-CDs) were used as a FRET ratiometric fluorescent probe for the detection of Cu 2+ in aqueous solution. 388 Similarly, Chen et al also applied cyclam-functionalized CDs as a FRET nanoprobe for the detection of Cu 2+ and S. 2–389 In addition to the above examples, the detection of other important metal ions such as Ag + , Cu 2+ , Cr 6+ , and Pb 2+ has also been successfully realized with CD composite-based sensing platforms. 390–401…”

Carbon Dot Nanoparticles: Exploring the Potential Use for Gene Delivery in Ophthalmic Diseases

“…Due to the optical property of C-dots, better efficiency in the transfection process was observed by cell imaging and real-time detection using confocal laser scanning microscopy (CLSM) assay. Another study was done by Chen et al [9], prepared CDs from low molecular weight PEI and modified them with various hydrophobic chains and different degrees of substitution. Analytical approaches like 1 H NMR spectroscopy, FT-IR spectroscopy, TEM, and XPS were used to confirm modification and substitution.…”

“…Due to small size capacity, high production costs, high immunogenicity, inflammatory responses, and invasive administration to the retina, viral gene delivery raises concerns [3]. This brings up basic research on non-viral vectors, such as different kind of inorganic nanoparticles (gold nanoparticles [4], magnetic nanoparticles [5], carbon nanotubes [6], nanodiamonds [7], graphene [8]) for nucleic acid delivery [9]. Moreover, NPs overcome various safety concerns as compared to viral vectors, such as cost-effectiveness, ease to customize, design, and optimize for cellular uptake, large DNA capacity which is a very critical factor to deliver large ocular disease gene.…”

pH sensing and bioimaging using green synthesized carbon dots from black fungus

“…The as-prepared CDs demonstrated excitation-dependent FL behavior, which approximates to reported literatures of CDs. 24 Increasing the excitation wavelength from 320 nm to 420 nm, the emission peak red-shifts gradually. At 370 nm excitation, the CDs show highest intensity at emission peak of 450 nm (Fig.…”