Hydrophobic Interaction Chromatography

Tomaz, Cândida T.; Queiroz, João A.

doi:10.1016/b978-0-12-415807-8.00006-7

Cited by 9 publications

(1 citation statement)

References 79 publications

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“…28 For separation processes, monoliths act as stationary phases, as the well-distributed nanometer- or micrometer-sized pores enhance the surface area of the monolith and allow the trapping of molecules. 29 Typically, the monolith is thermally polymerized by monomers to form a porous structure with high mechanical stability. 30 Since many monomers are capable of forming monoliths with porous structures, there is high flexibility in choosing suitable monomers according to the final application.…”

Section: Introductionmentioning

confidence: 99%

Monolith-modified cellulose paper for biochemical sensing applications

et al. 2022

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Section: Introductionmentioning

confidence: 99%

Monolith-modified cellulose paper for biochemical sensing applications

et al. 2022

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Hydrophobic Interaction Chromatography in 2 D Liquid Chromatography Characterization of Lignosulfonates

et al. 2020

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Lignosulfonates are bulk‐scale byproducts of industrial sulfite pulping. Their amphiphilic character plays a central role in their successful application in large‐scale materials production. As an inherent feature of the chemical structure, this amphiphilic character poses a major analytical challenge. In this study, the amphiphilic behavior of an industrial lignosulfonate was investigated by hydrophobic interaction chromatography (HIC). This technique exploits hydrophobic regions present on the surface of lignosulfonates. Extensive characterization of the obtained fractions from preparative HIC, in terms of elemental composition, functional‐group content, chemical structure, and molecular weight distribution, revealed a detailed picture of the chemical composition distribution. The charge‐to‐size ratio, that is, differences in the degree of sulfonation, was the dominant factor governing separation in HIC. A combination of HIC with size exclusion chromatography showed good orthogonality of separation and demonstrated the power of this 2 D liquid chromatography approach for an in‐depth characterization, in general, and amphiphilicity, in particular.

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ADC Analysis by Hydrophobic Interaction Chromatography

Fleming

2019

Methods in Molecular Biology

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Hydrophobic Interaction Chromatography

Cited by 9 publications

References 79 publications

Monolith-modified cellulose paper for biochemical sensing applications

Monolith-modified cellulose paper for biochemical sensing applications

Hydrophobic Interaction Chromatography in 2 D Liquid Chromatography Characterization of Lignosulfonates

ADC Analysis by Hydrophobic Interaction Chromatography

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