Hydrophilic polydopamine-derived mesoporous channels for loading Ti(IV) ions for salivary phosphoproteome research

Recent developments in mesoporous polydopamine-derived nanoplatforms for cancer theranostics

“…However, the preparation of porous carbon materials by the soft templating method also faces problems, such as low yield and low availability of template agents. Recently, the soft templating method has been widely used in the preparation of MPDA nanoplatforms [ 62 – 64 ]. Tang et al [ 52 ] reported the synthesis of highly nitrogen-doped mesoporous carbon spheres (NMCS).…”

Development of new lanthanide(iii) ion-based magnetic affinity materials for phosphopeptide enrichment

“…41 They prepared the newly designed Ti 4+ -based magnetic IMAC material by binding Ti 4+ ions to adenosine triphosphate (ATP) on the surface of magnetic nanoparticles (MNPs) and found higher sensitivity and selectivity for phosphopeptides in mouse liver mitochondria compared to conventional Ti-IMAC and TiO 2 microbeads. In recent studies, [53][54][55] different types of magnetic IMAC materials containing polydopamine (PDA) as a linker have been developed based on the strong affinity of Ti 4+ ions to catechol groups. These materials exhibited highly sensitive and selective behavior for the phosphoproteomic analyses in standard proteins and also exhibited real examples such as human salivary and HeLa cells, on comparing with the commercial TiO 2 based materials.…”

“…These materials exhibited highly sensitive and selective behavior for the phosphoproteomic analyses in standard proteins and also exhibited real examples such as human salivary and HeLa cells, on comparing with the commercial TiO 2 based materials. [53][54][55] In another study to develop a new chelating agent for the sensitive and selective phosphopeptide enrichment, Zhai et al (2014) prepared a magnetic IMAC material using DOTA (1,4,7,10-tetraazacyclododecane N,N,N,N-tetraacetic acid) as a chelating agent, which is known to be highly stable in complexes with metals and shown to be suitable for protein speciation studies. 2,3 Here, they used TCPP [tetrakis (4-carboxylphenyl) porphyrin] and ethylene diamine to bind DOTA to amine-modified magnetic nanoparticles and immobilized different lanthanide(III) ions to the surface.…”

Magnetic mesoporous silica nanoparticles modified by phosphonate functionalized ionic liquid for selective enrichment of phosphopeptides

“…[16][17][18] Besides, magnetic materials have also drawn much attention due to the rapid magnetic responsiveness that can be used to accelerate separation process. [19][20][21][22][23] Separation and enrichment by nonmagnetic materials is a time-consuming procedure usually including sample loading, centrifuging, washing and eluting; thereby tendency of contamination and loss of target molecules in low abundance is increased. 24 Magnetic nanomaterials and mesoporous materials with suitable surface modication can enrich phosphopeptides and accelerate the separation process in short time with the aid of external magnetic eld.…”