Hydrophilic meso-substituted cyanine dyes in solution and in complexes with serum albumins: spectral properties and molecular docking study

Пронкин, П. Г.; Шведова, Л. А.; Татиколов, А. С.

doi:10.1007/s12039-020-01858-2

Cited by 4 publications

(3 citation statements)

References 28 publications

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“…As to HSA, it is known that the formation of complexes with serum albumins generally proceeds more efficiently for anionic cyanine dyes than for cationic ones [ 64 , 65 , 66 , 67 ]. This may explain lower K eff values obtained for complexation of cationic MCD 1 and 4 with HSA than with dsDNA.…”

Section: Resultsmentioning

confidence: 99%

Photonics of Some Monomethine Cyanine Dyes in Solutions and in Complexes with Biomolecules

Pronkin,

Tatikolov

2023

IJMS

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In search of new probes for biomolecules, the spectral fluorescent study of four monomethine cyanine dyes (MCD), both unsymmetrical and symmetrical, has been carried out in different organic solvents, in aqueous buffer solutions, and in the presence of DNA and HSA. The complexation of MCD with biomacromolecules leads to a steep growth of the fluorescence intensity. Complexes of MCD with dsDNA and HSA of various types were modeled in silico by molecular docking. Experiments on thermal dissociation of dsDNA in the presence of MCD showed the formation of intercalative complexes of MCD with DNA. Quenching of intrinsic fluorescence of HSA by MCD occurred with rate constants much higher than the diffusion limit, that is, in dye–HSA complexes. Effective constants of MCD complexation with the biomacromolecules were estimated. MCD 1 has the best characteristics as a possible fluorescent probe for dsDNA and can serve as a sensitive and selective probe for dsDNA in the presence of HSA. Photochemical properties of MCD complexed with DNA have been also studied. An increase in the quantum yield of the triplet states of MCD in complexes with DNA has been found, which may be important for using these dyes as potential candidates in photodynamic therapy.

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Section: Resultsmentioning

confidence: 99%

Photonics of Some Monomethine Cyanine Dyes in Solutions and in Complexes with Biomolecules

Pronkin,

Tatikolov

2023

IJMS

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“…A comparative study of the interaction of meso -alkyl-substituted anionic trimethine cyanine dyes 79 (OXEC), 80 (DMEC; Figure 17 ), and 52 (DMC) with HSA and BSA was performed in [ 79 ]. Interaction with albumins leads to the formation of complexes of trans -isomers of the dyes; however, in the case of BSA, the shift of the cis – trans isomeric equilibrium is incomplete.…”

Section: Influence Of Interaction With Proteins and Other Biomolecule...mentioning

confidence: 99%

“…Dyes 79 and 69 are also able to interact with the protein in the form of cis -isomers. Molecular docking of dye–HSA systems was carried out; the results correspond to the binding of 52 on the surface of domain I of HSA (dye 53 is probably able to bind to domain IIA of HSA) [ 78 , 79 ].…”

Section: Influence Of Interaction With Proteins and Other Biomolecule...mentioning

confidence: 99%

Photonics of Trimethine Cyanine Dyes as Probes for Biomolecules

Пронкин

Татиколов

2022

Molecules

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Cyanine dyes are widely used as fluorescent probes in biophysics and medical biochemistry due to their unique photophysical and photochemical properties (their photonics). This review is focused on a subclass of the most widespread and studied cyanine dyes—trimethine cyanines, which can serve as potential probes for biomolecules. The works devoted to the study of the noncovalent interaction of trimethine cyanine dyes with biomolecules and changing the properties of these dyes upon the interaction are reviewed. In addition to the spectral-fluorescent properties, elementary photochemical properties of trimethine cyanines are considered, including: photoisomerization and back isomerization of the photoisomer, generation and decay of the triplet state, and its quenching by oxygen and other quenchers. The influence of DNA and other nucleic acids, proteins, and other biomolecules on these properties is covered. The interaction of a monomer dye molecule with a biomolecule usually leads to a fluorescence growth, damping of photoisomerization (if any), and an increase in intersystem crossing to the triplet state. Sometimes aggregation of dye molecules on biomolecules is observed. Quenching of the dye triplet state in a complex with biomolecules by molecular oxygen usually occurs with a rate constant much lower than the diffusion limit with allowance for the spin-statistical factor 1/9. The practical application of trimethine cyanines in biophysics and (medical) biochemistry is also considered. In conclusion, the prospects for further studies on the cyanine dye–biomolecule system and the development of new effective dye probes (including probes of a new type) for biomolecules are discussed.

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Spectral-fluorescent and photochemical study of 6,6′-di(benzoylamino)trimethine cyanine dyes in solutions as possible probes for DNA

Пронкин

Татиколов

2023

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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Hydrophilic meso-substituted cyanine dyes in solution and in complexes with serum albumins: spectral properties and molecular docking study

Cited by 4 publications

References 28 publications

Photonics of Some Monomethine Cyanine Dyes in Solutions and in Complexes with Biomolecules

Photonics of Some Monomethine Cyanine Dyes in Solutions and in Complexes with Biomolecules

Photonics of Trimethine Cyanine Dyes as Probes for Biomolecules

Spectral-fluorescent and photochemical study of 6,6′-di(benzoylamino)trimethine cyanine dyes in solutions as possible probes for DNA

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