Hydrophilic interaction liquid chromatography in analysis of granisetron HCl and its related substances. Retention mechanisms and method development

Maksić, Jelena; Tumpa, Anja; Stajić, Ana; Jovanović, Marko; Rakić, Tijana; Jančić–Stojanović, Biljana

doi:10.1016/j.jpba.2016.02.010

Cited by 13 publications

(6 citation statements)

References 16 publications

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“…At the end, spline interpolation was once again tested to prove its applicability. It was implemented on the results of our previous study . In that study, granisetron and its 2 impurities were investigated in HILIC, and their retention mechanisms were assessed.…”

Section: Resultsmentioning

confidence: 99%

Modeling of HILIC retention behavior with theoretical models and new spline interpolation technique

Tumpa

Mišković

Stanimirović

et al. 2017

Journal of Chemometrics

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When it is taken into account that hydrophilic interaction liquid chromatography (HILIC) as an analytical method is relatively young compared with the other techniques, retention modeling could still bring scientifically valuable data to the field. Therefore, in this paper, olanzapine and its 8 impurities were selected as a test mixture, considering that they have never been analyzed in HILIC before. Their investigation on 4 different HILIC columns (bare silica, cyanopropyl, diol and zwitterionic) has been performed. The mixture of 9 structurally similar substances allows the examination of complex HILIC retention behavior depending on the chemical properties of the analytes, as well as of the stationary phase. To describe the nature of the relationship between the retention and the stronger eluent content in the mobile phase, we fitted experimentally obtained data to several theoretical (localized adsorption, nonlocalized partition, quadratic, and mixed) models. Results show that the best fit is the quadratic model with the highest R 2 and cross-validated coefficient of determination (Q 2 ) values, but its usage has some drawbacks. With the aim to improve the possibility to predict retention behavior in HILIC, a new empirical model was proposed. For that purpose, a spline interpolation technique was performed, by dividing the experimental range into several subdivisions. This type of interpolation was performed for the first time in the chromatographic field.The estimation of the polynomial equations was performed using Q 2 values. Obtained Q 2 values pointed out the goodness of fit of the model, as well as its good predictive capabilities. In the end, the prediction capabilities were experimentally verified, under randomly chosen conditions from the experimental range. The errors in prediction were all under 10%, which is satisfying for HILIC.

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Section: Resultsmentioning

confidence: 99%

Modeling of HILIC retention behavior with theoretical models and new spline interpolation technique

Tumpa

Mišković

Stanimirović

et al. 2017

Journal of Chemometrics

Self Cite

View full text Add to dashboard Cite

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“…Recently, papers have focused on the optimization of a complete method, looking at different parameters such as buffers, salts, their concentrations, pH, the organic‐modifier content, temperature, and stationary phase. When optimizing the modifier content, a number of studies used the LSS model [131–135], the ADS model [100,131–136], the Q model [100,136], and the MM model [131,133]. Often, more than one model is used to distinguish for example between the partitioning mechanism and the adsorption mechanism [110,111].…”

Section: Methodsmentioning

confidence: 99%

Recent applications of retention modelling in liquid chromatography

Uijl

Schoenmakers

Pirok

et al. 2020

J of Separation Science

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Recent applications of retention modelling in liquid chromatography (2015–2020) are comprehensively reviewed. The fundamentals of the field, which date back much longer, are summarized. Retention modeling is used in retention‐mechanism studies, for determining physical parameters, such as lipophilicity, and for various more‐practical purposes, including method development and optimization, method transfer, and stationary‐phase characterization and comparison. The review focusses on the effects of mobile‐phase composition on retention, but other variables and novel models to describe their effects are also considered. The five most‐common models are addressed in detail, i.e. the log‐linear (linear‐solvent‐strength) model, the quadratic model, the log–log (adsorption) model, the mixed‐mode model, and the Neue–Kuss model. Isocratic and gradient‐elution methods are considered for determining model parameters and the evaluation and validation of fitted models is discussed. Strategies in which retention models are applied for developing and optimizing one‐ and two‐dimensional liquid chromatographic separations are discussed. The review culminates in some overall conclusions and several concrete recommendations.

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“…For example, retention behavior of granisetron and its two related substances with similar structure (Fig. ) on ZIC HILIC column was assessed, whereby kept the other parameters constant but changed the acetonitrile content (80–97%) . By fitting of the retention data to the above mentioned two theoretical models, it was found that although coefficients of determination ( R 2 ), which was used as measures of goodness of fit, were considerably high (0.9966–0.9989 and 0.9966–0.9987) in both models, the ranges of the values of standard errors regarding partition model and adsorption model were close (0.085–0.141 and 0.010–0.338), and in that case it would be not possible to determine which of the processes contribute to the overall HILIC retention mechanism most.…”

Section: Retention Mechanism and Retention Behavior In Hilicmentioning

confidence: 99%

Recent applications of hydrophilic interaction liquid chromatography in pharmaceutical analysis

Zhang

et al. 2016

J of Separation Science

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Hydrophilic interaction liquid chromatography, an alternative liquid chromatography mode, is of particular interest in separating hydrophilic and polar ionic compounds. Compared with traditional liquid chromatography techniques, hydrophilic interaction liquid chromatography offers specific advantages mainly including: (1) relatively green and water-soluble mobile phase composition, which enhances the solubility of hydrophilic and polar ionic compounds; (2) no need for ion-pairing reagents and high content of organic solvent, which benefits mass spectrometry detection; (3) high orthogonality to reverse-phase liquid chromatography, well adapted to two-dimensional liquid chromatography for complicated samples. Therefore, hydrophilic interaction liquid chromatography has been rapidly developed in many areas over the past decades. This review summarizes the recent progress (from 2012 to July 2016) of hydrophilic interaction liquid chromatography in pharmaceutical analysis, with the focus on detecting chemical drugs in various matrices, charactering active compounds of natural products and assessing biotherapeutics through typical structure unit. Moreover, the retention mechanism and behavior of analytes in hydrophilic interaction liquid chromatography as well as some novel hydrophilic interaction liquid chromatography columns used for pharmaceutical analysis are also described.

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Hydrophilic interaction liquid chromatography in analysis of granisetron HCl and its related substances. Retention mechanisms and method development

Cited by 13 publications

References 16 publications

Modeling of HILIC retention behavior with theoretical models and new spline interpolation technique

Modeling of HILIC retention behavior with theoretical models and new spline interpolation technique

Recent applications of retention modelling in liquid chromatography

Recent applications of hydrophilic interaction liquid chromatography in pharmaceutical analysis

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