Hydrophilic interaction chromatography combined with tandem mass spectrometry method for the quantification of tobramycin in human plasma and its application in a pharmacokinetic study

Chen, Lingyun; Chen, Huashan; Shen, Mei

doi:10.1016/j.jchromb.2014.10.007

Cited by 14 publications

(4 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The mobile phase was operated using a concentration gradient for methanol, ranging from 5% to 80%. The analytical method for tobramycin was similar to that used in other studies [27], [28], [29].…”

Section: Methodsmentioning

confidence: 99%

Recovery rates of combination antibiotic therapy using in vitro microdialysis simulating in vivo conditions

Dhanani

Parker

Lipman

et al. 2018

Journal of Pharmaceutical Analysis

View full text Add to dashboard Cite

Microdialysis is a technique used to measure the unbound antibiotic concentration in the interstitial spaces, the target site of action. In vitro recovery studies are essential to calibrating the microdialysis system for in vivo studies. The effect of a combination of antibiotics on recovery into microdialysate requires investigation. In vitro microdialysis recovery studies were conducted on a combination of vancomycin and tobramycin, in a simulated in vivo model. Comparison was made between recoveries for three different concentrations and three different perfusate flow rates. The overall relative recovery for vancomycin was lower than that of tobramycin. For tobramycin, a concentration of 20μg/mL and flow rate of 1.0μL/min had the best recovery. A concentration of 5.0μg/mL and flow rate of 1.0μL/min yielded maximal recovery for vancomycin. Large molecular size and higher protein binding resulted in lower relative recoveries for vancomycin. Perfusate flow rates and drug concentrations affected the relative recovery when a combination of vancomycin and tobramycin was tested. Low perfusate flow rates were associated with higher recovery rates. For combination antibiotic measurement which includes agents that are highly protein bound, in vitro studies performed prior to in vivo studies may ensure the reliable measurement of unbound concentrations.

show abstract

Section: Methodsmentioning

confidence: 99%

Recovery rates of combination antibiotic therapy using in vitro microdialysis simulating in vivo conditions

Dhanani

Parker

Lipman

et al. 2018

Journal of Pharmaceutical Analysis

View full text Add to dashboard Cite

show abstract

“…The mobile phase was 0.1% formic acid solution in water (90%) and 0.1% formic acid solution in acetonitrile (10%) with a flow rate of 0.6 mL/min. A gradient elution procedure was used, and each component was measured via multiple reaction monitoring (MRM) in the positive ionization mode (Table 1) [29]. Polymyxin B distribution in the Calu-3 cell monolayer was investigated using confocal laser scanning microscopy (CLSM) [26].…”

Section: Lc-ms Analysismentioning

confidence: 99%

Tobramycin Reduces Pulmonary Toxicity of Polymyxin B via Inhibiting the Megalin-Mediated Drug Uptake in the Human Lung Epithelial Cells

Ahmed,

Li,

Zhou

2024

Pharmaceutics

View full text Add to dashboard Cite

Accumulation of polymyxins in the lung epithelial cells can lead to increased mitochondrial oxidative stress and pulmonary toxicity. Aminoglycosides and polymyxins are used, via intravenous and pulmonary delivery, against multidrug-resistant Gram-negative pathogens. Our recent in vitro and animal studies demonstrated that the co-administration of polymyxins with aminoglycosides decreases polymyxin-induced pulmonary toxicity. The aim of this study was to investigate the in vitro transport and uptake of polymyxin B and tobramycin in human lung epithelial Calu-3 cells and the mechanism of reduced pulmonary toxicity resulting from this combination. Transport, intracellular localization, and accumulation of polymyxin B and tobramycin were investigated using doses of 30 mg/L polymyxin B, 70 mg/L tobramycin, and the combination of both. Adding tobramycin significantly (p < 0.05) decreased the polymyxin B-induced cytotoxicity in Calu-3 cells. The combination treatment significantly reduced the transport and uptake of polymyxin B and tobramycin in Calu-3 cells, compared to each drug alone, which supported the reduced pulmonary toxicity. We hypothesized that cellular uptake of polymyxin B and tobramycin shared a common transporter, megalin. We further investigated the megalin expression of Calu-3 cells using confocal microscopy and evaluated megalin activity using a megalin substrate, FITC-BSA, and a megalin inhibitor, sodium maleate. Both polymyxin B and tobramycin significantly inhibited FITC-BSA uptake by Calu-3 cells in a concentration-dependent manner. Sodium maleate substantially inhibited polymyxin B and tobramycin transport and cellular accumulation in the Calu-3 cell monolayer. Our study demonstrated that the significantly reduced uptake of polymyxin B and tobramycin in Calu-3 cells is attributed to the mechanism of action that determines that polymyxin B and tobramycin share a common transporter, megalin.

show abstract

“…The result indicated that the levels of free drug remained below levels that were regarded as safe [bound 1.63 mg/mL ± 1.48%, unbound 0.0671 mg/mL ± 1.68% (n = 5)]. Fast, sensitive, and specific determination of anastrozole (nonsteroidal aromatase inhibitor) [51], carboplatin (platinum compound used for treatment of nonsmall-cell lung cancer and ovarian cancer) [52], tobramycin (broad spectrum aminoglycoside antibiotic) [53], vindesine (tubulin mitotic function inhibitor) [54], metformin [55] in plasma with HILIC-MS/MS method were obtained and the corresponding method was applied to clinical/preclinical pharmacokinetic studies of the drug in healthy volunteers/patients/animals, respectively. Pharmacokinetic studies of different drugs with HILIC method, in fact, were following similar procedure.…”

Section: Plasma and Serum Samplesmentioning

confidence: 99%

Recent applications of hydrophilic interaction liquid chromatography in pharmaceutical analysis

Zhang

et al. 2016

J of Separation Science

View full text Add to dashboard Cite

Hydrophilic interaction liquid chromatography, an alternative liquid chromatography mode, is of particular interest in separating hydrophilic and polar ionic compounds. Compared with traditional liquid chromatography techniques, hydrophilic interaction liquid chromatography offers specific advantages mainly including: (1) relatively green and water-soluble mobile phase composition, which enhances the solubility of hydrophilic and polar ionic compounds; (2) no need for ion-pairing reagents and high content of organic solvent, which benefits mass spectrometry detection; (3) high orthogonality to reverse-phase liquid chromatography, well adapted to two-dimensional liquid chromatography for complicated samples. Therefore, hydrophilic interaction liquid chromatography has been rapidly developed in many areas over the past decades. This review summarizes the recent progress (from 2012 to July 2016) of hydrophilic interaction liquid chromatography in pharmaceutical analysis, with the focus on detecting chemical drugs in various matrices, charactering active compounds of natural products and assessing biotherapeutics through typical structure unit. Moreover, the retention mechanism and behavior of analytes in hydrophilic interaction liquid chromatography as well as some novel hydrophilic interaction liquid chromatography columns used for pharmaceutical analysis are also described.

show abstract

Hydrophilic interaction chromatography combined with tandem mass spectrometry method for the quantification of tobramycin in human plasma and its application in a pharmacokinetic study

Cited by 14 publications

References 21 publications

Recovery rates of combination antibiotic therapy using in vitro microdialysis simulating in vivo conditions

Recovery rates of combination antibiotic therapy using in vitro microdialysis simulating in vivo conditions

Tobramycin Reduces Pulmonary Toxicity of Polymyxin B via Inhibiting the Megalin-Mediated Drug Uptake in the Human Lung Epithelial Cells

Recent applications of hydrophilic interaction liquid chromatography in pharmaceutical analysis

Contact Info

Product

Resources

About