Hydrophilic Interaction Chromatography Analysis of Esculin in Ointments with UV Detection

Abdulla, Fatma H.; Rasheed, Ashraf Saad

doi:10.2174/1573412916666200316124837

Cited by 8 publications

(3 citation statements)

References 24 publications

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“…The mobile phase was filtered with 0.45 µm filters. The first-dimension column utilized a home-made ZIC-HILIC column, ZIC-SB5 (100 mm x 4.6 mm) 47 Ȧ, particle size 3.5 µm, following the reference [38][39][40]. while the second-dimension column was obtained from Nouryon-Kromasil (Sweden), RP column, Kromasil C18 (50 mm x 3.0 mm id) 100 Ȧ, particle size 5µm.…”

Section: Methodsmentioning

confidence: 99%

Online Two-Dimensional Liquid Chromatography for Determination of Selected Flavonoids in Wine Samples

Abdulmehdi,

Rasheed

2024

Iraqi Journal of Science

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The development of two-dimensional chromatography systems is crucial in many scientific fields as it provides improved separation efficiency and selectivity of complex mixtures. By separating compounds based on two independent properties, these systems can offer a more detailed understanding of sample composition. This increased level of detail is particularly useful in industries such as pharmaceuticals, environmental analysis, and proteomics, where complex mixtures with similar properties need to be differentiated. A new chromatography system with two dimensions was developed to separate and quantify specific flavonoids in German wine samples. The first dimension used a homemade hydrophilic interaction liquid chromatographic column, while the second dimension used a reversed-phase liquid chromatographic column. This 2D ZIC-HILIC x RP technique was able to address the problem of peak overlapping that commonly occurs in one-dimensional liquid chromatography, especially for natural products. The study presents analytical and statistical data for both techniques, and suggests that the use of two-dimensional chromatography can improve the resolution of conventional one-dimensional liquid chromatography. In a conventional ZIC-HILIC-HPLC system, apigenin and naringenin were detected together but were successfully separated and measured using a 2DLC system. The limits of detection (LOD) for apigenin and naringenin were found to be 0.0705 µg/mL and 0.1300 µg/mL, respectively, while the limits of quantification (LOQ) were 0.2136 µg/mL and 0.3939 µg/mL, respectively. The recovery rate for both compounds was over 99%, with a relative standard deviation (RSD) of less than 1.5%. The linearity range for both compounds was between 0.005 µg/mL to 10 µg/mL, and the correlation coefficient (r2) was determined to be 0.9998. For hesperidin and rutin, the LOD and LOQ were found to be 0.0336 µg/mL and 0.1018 µg/mL, and 0.0120 µg/mL and 0.0363 µg/mL, respectively. The linearity range for both compounds was between 0.005 µg/mL to 12 µg/mL, and the correlation coefficient (r2) was determined to be 0.9999.

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Section: Methodsmentioning

confidence: 99%

Online Two-Dimensional Liquid Chromatography for Determination of Selected Flavonoids in Wine Samples

Abdulmehdi,

Rasheed

2024

Iraqi Journal of Science

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“…Additionally, the influence of the chain longitude of the ZIC-HILIC columns on ketoprofen retention behaviour has not been examined previously. Rasheed and colleagues [19,27] have investigated the influence of chain length on HILIC columns and its effect on the Ranitidine and esculin behaviour. They discovered that the longer the chain length, the more interaction between the Ranitidine and esculin and the ZIC-HILIC column occurs, resulting in a longer retention time.…”

Section: Introductionmentioning

confidence: 99%

Quantification of ketoprofen in film-coated tablets using hydrophilic interaction liquid chromatography-Ultraviolet spectrometry

2022

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A simple zwitterionic hydrophilic interaction liquid chromatography (ZIC-HILIC) method was developed and applied to filmcoated tablets to determine ketoprofen. Separation was performed using gradient elution with a mixture of acetonitrile and acetate buffer at an Ultraviolet (UV) detection wavelength of 255 nm on self-constructed ZIC-HILIC-1 and ZIC-HILIC-4 columns. The hydrophilicity of ketoprofen was validated by examining its behaviour with varied acetate buffer, acetonitrile proportions, and pH values. The mechanism of separation is based on ketoprofen partitioning in the hydrophobic interaction. The two available approaches are a viable alternative to the present ketoprofen separation methods. The developed ZIC-HILIC methods had an excellent precision (0.61%), linear ranges of 0.05-10 ppm, and detection limits of 0.01-0.008 ppm with a coefficient of 0.9995 0.9997 for the ZIC-HILIC-1 and ZIC-HILIC-4 columns, respectively. The methods' findings were compared to the British Pharmacopoeia procedure using statistical testing, which revealed no difference in accuracy between the methods.

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“…Rasheed and his team have presented numerous studies employing the HILIC technique in various applications, and are mentioned in the following references. [11][12][13][14][15][16][17][18][19][20][21]…”

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confidence: 99%

Knowledge of The HILIC Retention Behaviors of Two Quinolone Antibiotics on Sulfobetaine-Type Zwitterionic Stationary Phases

Al-Aphalahy¹,

Qassim²,

Karabat³

2023

IJDDT

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Fluoroquinolones treat infections of the urinary, respiratory, gastrointestinal, skin, bone, and joint systems. This article describes the development of the hydrophilic interaction liquid chromatography (ZIC-HILIC) method to determine norfl oxacin and ofl oxacin retention characteristics. Separation of all analytes was accomplished using ZIC-HILIC1 and ZIC-HILIC4 columns. The mobile phase was 40 mM sodium acetate water solution in acetonitrile pumped at a 0.7 mL/min fl ow rate. The infl uence of acetonitrile concentration, the presence of salt in the mobile phase, and pH on the retention of norfl oxacin and ofl oxacin in the HILIC mode were investigated in this study. When using ZIC-HILIC columns, it was discovered that the retention factors of the analytes were inversely proportional to the amount of water present in the mobile phase, which is representative of a conventional hydrophilic partitioning mechanism. The ZIC-HILIC based strategies described in this article represent a signifi cant step toward more sensitive drug analysis. We observed that zwitterionic materials could govern the separation of materials, as shown by the infl uence of the chain length between the zwitterion groups, which contributed to the explanation of the variation in the retention period of the materials to be separated.

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Hydrophilic Interaction Chromatography Analysis of Esculin in Ointments with UV Detection

Cited by 8 publications

References 24 publications

Online Two-Dimensional Liquid Chromatography for Determination of Selected Flavonoids in Wine Samples

Online Two-Dimensional Liquid Chromatography for Determination of Selected Flavonoids in Wine Samples

Quantification of ketoprofen in film-coated tablets using hydrophilic interaction liquid chromatography-Ultraviolet spectrometry

Knowledge of The HILIC Retention Behaviors of Two Quinolone Antibiotics on Sulfobetaine-Type Zwitterionic Stationary Phases

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