Hydrophilic interaction chromatography

Gama, Mariana R.; Silva, Raquel Gomes da Costa; Collins, Carol H.; Bottoli, Carla Beatriz Grespan

doi:10.1016/j.trac.2012.03.009

Cited by 145 publications

(74 citation statements)

References 96 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The types of stationary phase matrices, column packing methods, eluent compositions, and detection modes vary between IC and HPLC. IC variants, such as ion-exclusion chromatography (IEC), ion-pair chromatography (IPC), and reversed-phase liquid chromatography (RPLC), have also been used to separate hydrophilic molecules, biomolecules, and metal complexes (Fritz 1991;Gama et al 2012;Gennaro and Angelino 1997;Michalski 2014;Nakatani et al 2012). In tandem with mass spectrometric (MS) detection, as implemented in HPLC-MS, LC-MS, and LC-MS/MS, more complex water-soluble organic compositions, molecular structures, molar weights, and functional groups (Fenn 2002(Fenn , 2003Michalski 2014) can be quantified at ultra-low detection limits.…”

Section: Recent Reviews Of Liquid Chromatography (Lc) Methodsmentioning

confidence: 99%

Enhanced Ion Chromatographic Speciation of Water-Soluble PM$$_{2.5}$$ to Improve Aerosol Source Apportionment

Chow

Watson

2017

Aerosol Sci Eng

View full text Add to dashboard Cite

Ion chromatography (IC) is widely used to quantify sulfate, nitrate, ammonium, sodium, chloride, and potassium from PM 2:5 water extracts. IC hardware and software have progressed to allow a broader range of water-soluble compounds to be determined for the existing anion and cation programs and on the same solutions using analytical column, eluent, and detector modifications. Alkylamine, organic acid, and carbohydrate quantification by IC expands the number of source markers, especially for different types of biomass burning and secondary organic aerosols. Although modern systems are highly automated, internal quality control (QC) and external quality assurance (QA) programs are essential. QC includes detailed standard operating procedures, calibration over the range of expected concentrations, performance tests with independent standards, inspection of filters and chromatograms, and anion/cation balances. QA consists of independent system and performance audits, analysis of externally prepared performance samples, and interlaboratory comparisons. The additional water-soluble species provide compounds for speciated emission inventories, source markers to refine aerosol source apportionment, and increased understanding of global carbon, sulfur, and nitrogen cycles.

show abstract

Section: Recent Reviews Of Liquid Chromatography (Lc) Methodsmentioning

confidence: 99%

Enhanced Ion Chromatographic Speciation of Water-Soluble PM$$_{2.5}$$ to Improve Aerosol Source Apportionment

Chow

Watson

2017

Aerosol Sci Eng

View full text Add to dashboard Cite

show abstract

“…The reported formate concentrations were the final concentrations in the mobile phase. Because of its low viscosity [44][45][46][47][48] , MeCN was used as the organic component of the mobile phase. The chromatographic results presented in this study were for the optimized parameters and for those columns where a separation was observed.…”

Section: Optimization Of Column Parametersmentioning

confidence: 99%

“…45,47,56,[60][61][62][63] Studies by Li and Huang 62 showed better separation when MeCN was used as the mobile phase compared to MeOH, THF and isopropanol. Attempts by Dinh et al 58 to admixture THF with the MeCN-containing mobile phase reduced the extent of formation of the water enriched layer on the stationary phase.…”

Section: The Moderately Polar Aprotic Diluent For Analyte Injectionmentioning

confidence: 99%

Comparative study of different column types for the separation of polar basic hallucinogenic alkaloids

Ncube¹,

Poliwoda²,

Cukrowska³

et al. 2016

S.Afr.j.chem.

View full text Add to dashboard Cite

The number of hallucinogenic compounds that have been separated simultaneously by liquid chromatography is limited. This research aimed to identify a column(s) that can allow for separation of several hallucinogens. The extent of separation of seven polar hallucinogenic tryptamine and phenethylamine derived alkaloids containing a basic N atom that becomes protonated at low pH values were investigated on five reverse-based columns and one hydrophilic interaction liquid chromatography (HILIC) column. An RP-phenyl and a negatively charged fused core HILIC were identified and recommended as effective columns in this regard. This research is the first to introduce a HILIC column in separation of hallucinogenic alkaloids and to simultaneously study the separation of the two principal psychoactive agents, muscimol and psilocin. The results of this study showed that better separation in HILIC columns can be achieved if an aprotic diluent of relatively lower polarity is used to introduce analytes into a mobile phase of higher polarity. Tetrahydrofuran was recommended where acetonitrile is used as the organic component of the mobile phase.

show abstract

“…Since its introduction by Alpert in 1990 [101], HILIC mode has been increasingly applied. There are several review articles giving exhaustive description of HILIC applications [102][103][104][105][106].…”

Section: Hydrophilic Interaction Modementioning

confidence: 99%

Versatile ligands for high-performance liquid chromatography: An overview of ionic liquid-functionalized stationary phases

Zhang

Mallik

Takafuji

et al. 2015

Analytica Chimica Acta

View full text Add to dashboard Cite

Hydrophilic interaction chromatography

Cited by 145 publications

References 96 publications

Enhanced Ion Chromatographic Speciation of Water-Soluble PM$$_{2.5}$$ to Improve Aerosol Source Apportionment

Enhanced Ion Chromatographic Speciation of Water-Soluble PM$$_{2.5}$$ to Improve Aerosol Source Apportionment

Comparative study of different column types for the separation of polar basic hallucinogenic alkaloids

Versatile ligands for high-performance liquid chromatography: An overview of ionic liquid-functionalized stationary phases

Contact Info

Product

Resources

About