Hydrophilic immobilized trypsin reactor with magnetic graphene oxide as support for high efficient proteome digestion

Jiang, Bo; Yang, Kaiguang; Zhao, Qun; Wu, Qi; Liang, Zhen; Zhang, Lihua; Peng, Xiaojun; Zhang, Yukui

doi:10.1016/j.chroma.2012.07.030

Cited by 85 publications

(28 citation statements)

References 48 publications

(50 reference statements)

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“…The functionalization of magnetic nanoparticles with carbon-based nanomaterials has recently attracted great interest as the resulting hybrid nanomaterials combine the properties of both building blocks. These hybrids can be applied in enzyme immobilization (Jiang et al, 2012;Gokhale et al, 2013) as well as in drug delivery (Yang et al, 2009;Mahmoudi et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Hybrid Nanomaterials of Magnetic Iron Nanoparticles and Graphene Oxide as Matrices for the Immobilization of β-Glucosidase: Synthesis, Characterization, and Biocatalytic Properties

et al. 2018

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Hybrid nanostructures of magnetic iron nanoparticles and graphene oxide were synthesized and used as nanosupports for the covalent immobilization of β-glucosidase. This study revealed that the immobilization efficiency depends on the structure and the surface chemistry of nanostructures employed. The hybrid nanostructure-based biocatalysts formed exhibited a two to four-fold higher thermostability as compared to the free enzyme, as well as an enhanced performance at higher temperatures (up to 70 • C) and in a wider pH range. Moreover, these biocatalysts retained a significant part of their bioactivity (up to 40%) after 12 repeated reaction cycles.

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Section: Introductionmentioning

confidence: 99%

Hybrid Nanomaterials of Magnetic Iron Nanoparticles and Graphene Oxide as Matrices for the Immobilization of β-Glucosidase: Synthesis, Characterization, and Biocatalytic Properties

et al. 2018

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“…The sequence coverage obtained from the database of MG@PDA‐trypsin nanocomposites is 62% for Cyt‐c and 83% for Myo, which is similar to those of other reported IMERs. For example, in Jiang's work, the sequence coverage obtained from the database of trypsin‐immobilized GO‐CO‐NH‐Fe 3 O 4 nanocomposites is 60% for Cyt‐c and 84% for Myo . The results indicate that trypsin immobilized MG@PDA nanocomposites are beneficial to improve the digestion efficiency.…”

Section: Methodsmentioning

confidence: 95%

Hydrophilic polydopamine‐coated magnetic graphene nanocomposites for highly efficient tryptic immobilization

Shi

Deng

et al. 2014

Proteomics

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In this work, polydopamine-coated magnetic graphene (MG@PDA) nanocomposites were synthesized by a facile method. Trypsin was then directly immobilized on the surface of the nanocomposites through simple PDA chemistry with no need for introducing any other coupling groups. The as-made MG@PDA nanocomposites inherit not only the large surface area of graphene which makes them capable of immobilizing high amount of trypsin (up to 0.175 mg/mg), but also the good hydrophilicity of PDA which greatly improves their biocompatibility. Moreover, the strong magnetic responsibility makes them easy to be separated from the digested peptide solution when applying a magnetic field. The feasibility of the trypsin-immobilized MG@PDA (MG@PDA-trypsin) nanocomposites for protein digestion was investigated and the results indicated their high digestion efficiency in a short digestion time (10 min). In addition, the reusability and stability of the MG@PDA-trypsin nanocomposites were also tested in our work. To further confirm the efficiency of MG@PDA-trypsin nanocomposites for proteome analysis, they were applied to digest proteins extracted from skimmed milk, followed by nano RPLC-ESI-MS/MS analysis, and a total of 321 proteins were identified, much more than those obtained by 16-h in-solution digestion (264 proteins), indicating the great potential of MG@PDA-trypsin nanocomposites as the supports for high-throughput proteome study.

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“…Carbon-encapsulated ZVI has shown better efficiency in the removal of phenol compared to bare ZVI . Graphene provides high surface area for attachment of catalyst due to its 2D structure (Zhang et al 2010;Jiang et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Effects of pH and Anions on the Generation of Reactive Oxygen Species (ROS) in nZVI-rGo-Activated Persulfate System

Ahmad

et al. 2015

Water Air Soil Pollut

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In this study, nanosized zero-valent iron-reduced graphene oxide (nZVI-rGO)-activated persulfate (PS) was used to investigate the generation of reactive oxygen species (ROS) for the degradation of trichloroethylene (TCE) in the aqueous solution. More than 98 % of TCE was degraded within 2 min under experimental conditions. The generation of ·OH increased when the pH was shifted toward the basic region while ·SO 4 − radicals' intensity increased in the acidic pH. Different scenarios have been observed in ·O 2 − generation in the neutral and strong basic pH and decreased in acidic or slightly basic pH. In addition, the intensity of ·OH was increased with the addition of HCO 3 − (10 mM) and NO 3 − (100 mM) but decreased in the presence of Cl − (10 and 100 mM), HCO 3 − (100 mM), and NO 3 − (10 mM). The degradation of anisole, probe for both ·OH and ·SO 4 − , was slightly enhanced by 10 mM NO 3 − anions but decreased in 100 mM salt solution. ·O 2 − intensity was increased while HCO 3 − (10 and 100 mM) and NO 3 − (100 mM) anions were used.nZVI-rGO-activated PS process could remove TCE in aqueous effectively, and the ROS generation and intensity were influenced by solution pH values and anions.

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Hydrophilic immobilized trypsin reactor with magnetic graphene oxide as support for high efficient proteome digestion

Cited by 85 publications

References 48 publications

Hybrid Nanomaterials of Magnetic Iron Nanoparticles and Graphene Oxide as Matrices for the Immobilization of β-Glucosidase: Synthesis, Characterization, and Biocatalytic Properties

Hybrid Nanomaterials of Magnetic Iron Nanoparticles and Graphene Oxide as Matrices for the Immobilization of β-Glucosidase: Synthesis, Characterization, and Biocatalytic Properties

Hydrophilic polydopamine‐coated magnetic graphene nanocomposites for highly efficient tryptic immobilization

Effects of pH and Anions on the Generation of Reactive Oxygen Species (ROS) in nZVI-rGo-Activated Persulfate System

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