Hydrophilic Fluorescent Nanogel Thermometer for Intracellular Thermometry

Gota, Chie; Okabe, Kimiko; Funatsu, Takashi; Harada, Yoshie; Uchiyama, Seiichi

doi:10.1021/ja807714j

Cited by 490 publications

(515 citation statements)

References 24 publications

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“…Thus, several approaches have been proposed to detect cellular temperature response using the emission intensity or lifetime of organic dyes (Chapman et al, 1995;Gota et al, 2009) and transition metal ions (Zohar et al, 1998;Suzuki et al, 2007). These pioneering works were able to provide the average temperature for individual cells.…”

Section: Approaches To Monitoring Of Intracellular Temperaturementioning

confidence: 99%

“…Among these emerging thermometers, which are based on lanthanide nanoparticles (Vetrone et a., 2010;Peng et al, 2010;Brites et al, 2010;Fisher et al, 2011), dye-coated nanoparticles (Huang et al, 2010), quantum dots (Yang et al, 2010), semiconducting polymer dots (Ye et al, 2011), temperature-responsive polymers (Chen & Chen, 2011;Gota et al, 2009) and a fluorescent nanogel thermometer (FNT) by combining a thermo-responsive polymer with a water-sensitive fluorophore (Okabe et al, 2012). To the point, the last is the only thermometer that allows the mapping of intracellular temperatures due to its ability to diffuse throughout living cells and its sensitivity to temperature.…”

Section: Approaches To Monitoring Of Intracellular Temperaturementioning

confidence: 99%

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Cell Thermoregulation: Problems, Advances and Perspectives

Ibraimov¹

2017

JMBR

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Thermoregulation at organism level is the well-established fact. The question on possibility of thermoregulation at the cell level remains opened. Based on study of distribution of chromosomal heterochromatin regions (HRs) in various human populations, in norm and at some forms of pathology the hypothesis about thermoregulation existence at the cell level has been presented. The essence of hypothesis of cell thermoregulation (СТ) is elimination of the temperature difference between the nucleus and cytoplasm when the nucleus temperature becomes higher than the cytoplasm temperature. The nucleus, in contrast to the cytoplasm, cannot conduct heat directly in the extracellular space, from where the heat is taken by the circulating flow of sap, lymph and blood. Thus, the nucleus can conduct heat only in the cytoplasm. With this, the nucleus has two options for the dissipation of heat surplus: either by increasing its volume or increasing the heat conductivity of the nuclear envelope. As the first option is limited, and the second one is hampered because of thickness of the cell membranes, apparently the higher eukaryotes took advantage of the opportunity of a dense layer of peripheral condensed chromatin (CC) as heat conductor for a more efficient elimination of the temperature difference between the nucleus and cytoplasm. The СС localized between a nucleus and cytoplasm is made of different types of chromosomal HRs. For this reason, СС is subject to wide variability in population. Obviously, the density of the СС packing depends on the type and quantity of chromosomal HRs in its structure that can affect upon its heat-conducting ability. If the situation is this then we are entitled to expect a new type of variability with all consequences resulting from here.

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Section: Approaches To Monitoring Of Intracellular Temperaturementioning

confidence: 99%

Section: Approaches To Monitoring Of Intracellular Temperaturementioning

confidence: 99%

Cell Thermoregulation: Problems, Advances and Perspectives

Ibraimov¹

2017

JMBR

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“…As the temperature is increased, the polymer shrinks, accompanied by the release of water molecules, resulting in fluorescence enhancement due to the BD units. Such nanosystems are applicable for the monitoring of temperature-dependent intracellular events, as demonstrated by studies with COS7 cells, in which environmental pH and surrounding proteins did not affect the performance of the polymer (Gota et al, 2009). Similarly, dual fluorescent micelles based on tetramethylrhodamine isothiocyanate and hostasol methacrylate have been synthesized, and were shown to be spontaneously taken up by HeLa cells .…”

Section: Synthesis Of Polymeric Nanoparticlesmentioning

confidence: 99%

Polymeric nanoparticles for optical sensing

Canfarotta

Whitcombe

Piletsky

2013

Biotechnology Advances

120

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Nanotechnology is a powerful tool for use in diagnostic applications. For these purposes a variety of functional nanoparticles containing fluorescent labels, gold and quantum dots at their cores have been produced, with the aim of enhanced sensitivity and multiplexing capabilities. This work will review progress in the application of polymeric nanoparticles in optical diagnostics, both for in vitro and in vivo detection, together with a discussion of their biodistribution and biocompatibility.

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“…Usually, the PNIPAM-based fluorescent hydro-gels, reported from other research groups, exhibit an on−off feature during the temperature change around the PNIPAM LCST. 1,6,9,[21][22][23]30 An on−off PL feature imposes limits on potential applications, compared to a continuously TR PL system.…”

Section: ■ Introductionmentioning

confidence: 99%

Interfacing a Tetraphenylethene Derivative and a Smart Hydrogel for Temperature-Dependent Photoluminescence with Sensitive Thermoresponse

Jiang

Yang

et al. 2014

ACS Appl. Mater. Interfaces

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Hydrophilic Fluorescent Nanogel Thermometer for Intracellular Thermometry

Cited by 490 publications

References 24 publications

Cell Thermoregulation: Problems, Advances and Perspectives

Cell Thermoregulation: Problems, Advances and Perspectives

Polymeric nanoparticles for optical sensing

Interfacing a Tetraphenylethene Derivative and a Smart Hydrogel for Temperature-Dependent Photoluminescence with Sensitive Thermoresponse

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