Hydrophilic electrospun polyurethane nanofiber matrices for hMSC culture in a microfluidic cell chip

Lee, Kwang Ho; Kwon, Gu Han; Shin, Suji; Baek, Jongduk; Han, Dong Keun; Park, Yongdoo; Lee, Sang Hoon

doi:10.1002/jbm.a.32059

Cited by 47 publications

(26 citation statements)

References 28 publications

(29 reference statements)

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“…Another interesting work reported that nanofiber-based extracellular matrix proteins can be used for patterning substrates of hMSCs. 149 Various types of nanostructures can be engineered to mimic microenvironments using submicron sized synthetic nanofibers. It was observed that the cells on the AA-grafted nanofibers greatly promoted the hMSC adhesion, migration, and proliferation when compared to untreated nanofibers.…”

Section: Patterning Structuresmentioning

confidence: 99%

Stem cells in microfluidics

Lin²,

Lee³

2011

Biomicrofluidics

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Microfluidic techniques have been recently developed for cell-based assays. In microfluidic systems, the objective is for these microenvironments to mimic in vivo surroundings. With advantageous characteristics such as optical transparency and the capability for automating protocols, different types of cells can be cultured, screened, and monitored in real time to systematically investigate their morphology and functions under well-controlled microenvironments in response to various stimuli. Recently, the study of stem cells using microfluidic platforms has attracted considerable interest. Even though stem cells have been studied extensively using bench-top systems, an understanding of their behavior in in vivo-like microenvironments which stimulate cell proliferation and differentiation is still lacking. In this paper, recent cell studies using microfluidic systems are first introduced. The various miniature systems for cell culture, sorting and isolation, and stimulation are then systematically reviewed. The main focus of this review is on papers published in recent years studying stem cells by using microfluidic technology. This review aims to provide experts in microfluidics an overview of various microfluidic systems for stem cell research.

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Section: Patterning Structuresmentioning

confidence: 99%

Stem cells in microfluidics

Lin²,

Lee³

2011

Biomicrofluidics

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“…4, 15, 28 Although 2D cell culture matrices such as collagen and fibronectin layers have been widely applied in culture flasks and microfluidic devices, little research has been done to incorporate in vivo representative ECM analogue fibers in a fluidic device. 22 Cells and tissues are embedded within 3D, fibrous ECM in vivo , which have proven to be able to regulate cellular activities. 29 In other words, even under flow conditions, if cells are not cultured on an ECM analogue scaffold, they may not be able to fully mimic in vivo conditions.…”

Section: Resultsmentioning

confidence: 99%

“…There are few reports showing the integration of electrospun fibers in a fluidic device, all of which are based upon sealing a layer of electrospun fibers between a substrate (on which the fibers are first sprayed) and a channel slab. 21, 22 In addition, with these approaches the electrospun fibers only reside on one side of a square channel, which limits the area and capacity for cell culture, as well as the creation of a true 3D ECM scaffold. Moreover, some techniques used in these reports, such as nano-gold electrode array 21 add to the cost and complexity of the device.…”

Section: Introductionmentioning

confidence: 99%

Use of electrospinning and dynamic air focusing to create three-dimensional cell culture scaffolds in microfluidic devices

Chen

Mehl

Sell

et al. 2016

Analyst

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Organs-on-a-chip has emerged as a powerful tool for pharmacological and physiological studies. A key part in the construction of such a model is the ability to pattern or culture cells in a biomimetic fashion. Most of the reported cells-on-a-chip models integrate cells on a flat surface, which does not accurately represent the extracellular matrix that they experience in vivo. Electrospinning, a technique used to generate sub-micron diameter polymer fibers, has been used as an in vitro cell culture substrate and for tissue engineering applications. Electrospinning of fibers directly into a fully sealed fluidic channel using a conventional setup has not been possible due to issues of confining the fibers into a discrete network. In this work, a dynamic focusing method was developed, with this approach enabling direct deposition of electrospun fibers into a fully sealed fluidic channel, to act as a matrix for cell culture and subsequent studies under continuous flowing conditions. Scanning electron microscopy of electrospun polycaprolactone fibers shows that this method enables the formation of fibrous layers on the inner wall of a 3D-printed fluidic device (mean fiber size = 1.6 ± 0.6 μm and average pore size = 113 ± 19 μm2). Cells were able to be cultured in this 3D scaffold without the addition of adhesion proteins. Media was pumped through the channel at high flow rates (up to 400 μL/min) during a dynamic cell culture process and both the fibers and the cells were found to be strongly adherent. A PDMS fluidic device was also prepared (from a 3D printed mold) and coated with polycaprolactone fibers. The PDMS device enables optical detection and confocal imaging of cultured cells on the fibers. Finally, macrophages were cultured in the devices to study how the fibrous scaffold can affect cell behavior. It was found that under lipopolysaccharide stimulation, macrophages cultured on PCL fibers inside of a channel secreted significantly more cytokines than those cultured on a thin layer of PCL in a channel or directly on the inner wall of a channel. Overall, this study represents a new approach and technique for in vitro cell studies, where electrospinning can be used to easily and quickly create 3D scaffolds that can improve the culture conditions in microfluidic devices.

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“…This phenomenon is not favorable in cell culture applications in most of the experiments because cells needed to adhere to the substrate. To address this problem, chemical or electrochemical methods have been used to render such scaffolds hydrophilic [15][16][17]. Lee et al [15] used AA-grafted nanofibers to treat the electrospun polyurethane nanofiber matrices surface.…”

Section: Introductionmentioning

confidence: 99%

“…To address this problem, chemical or electrochemical methods have been used to render such scaffolds hydrophilic [15][16][17]. Lee et al [15] used AA-grafted nanofibers to treat the electrospun polyurethane nanofiber matrices surface. The results confirmed that the contact angle of the AA-grafted treated surface was 10 times smaller than the untreated ones.…”

Section: Introductionmentioning

confidence: 99%

Plasma based surface modification of Poly (dimethylsiloxane) electrospun membrane proper for Organ On a Chip applications

et al. 2018

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Constructing of the scaffolds for cell culture applications has long been of interest for engineering researchers and biologist. In this study, a novel process is utilized for construction of suitable membrane with a high mechanical strength and appropriate surface behavior. Poly (dimethylsiloxane) (PDMS) is electrospun into fine fibers using poly (methyl methacrylate) (PMMA) as the carrier polymer in different weight ratios.Since the surface behavior of all PDMS substrates is moderately hydrophobic (120 < contact angle (CA) < 150), the electrospun membranes with higher PDMS ratios show slightly higher hydrophilicity. Direct plasma treatment is utilized to change the interfacial wettability of the membrane. Applying plasma changes the surface energy and renders the PDMS/PMMA substrates superhydrophilic (CA<5). In the following, the mechanical properties of the membrane are evaluated by the tensile test, and the results confirm the suitable mechanical strength of electrospun PDMS for cell culturing objectives (yield stress of more than 10 kPa). Also, changing the PDMS ratio doesn't significantly affect the total stiffness of the membrane. Thus, applying the proposed fabrication protocol provides a proper platform for cell viability and proliferation which have been proven by the results of MTT assay.2

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Hydrophilic electrospun polyurethane nanofiber matrices for hMSC culture in a microfluidic cell chip

Cited by 47 publications

References 28 publications

Stem cells in microfluidics

Stem cells in microfluidics

Use of electrospinning and dynamic air focusing to create three-dimensional cell culture scaffolds in microfluidic devices

Plasma based surface modification of Poly (dimethylsiloxane) electrospun membrane proper for Organ On a Chip applications

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