Hydrophilic, dual amino acid–functionalized zinc sulfide quantum dot for specific identification of N‐glycopeptides from biological samples

Abstract

translational modifications, and N-glycosylation plays a crucial role in life activities.Mass spectrometry (MS) has been a powerful technique in the analysis of protein glycosylation. However, the direct detection of glycoproteins in biological samples based on MS still suffers from huge challenges. Therefore, enrichment and purification of samples before MS analysis is an essential prerequisite.Methods: Hydrophilic interaction liquid chromatography (HILIC) has significantly developed for selective enrichment of glycopeptides due to its simple operation process and unbiased enrichment. Herein, hydrophilic, dual amino acidfunctionalized zinc sulfide quantum dots (ZnS QDs) were prepared to enrich glycopeptides using an easy procedure. The enriched glycopeptides were detected using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Results:The obtained material exhibited high selectivity (1:2000), low detection limit (0.1 fmol/μl), good repeatability (10 times), and excellent recovery (89.8%) in glycopeptide enrichment. In the actual application in biological samples, 71 Nglycopeptides and 161 N-glycopeptides were detected from human saliva and serum, respectively.Conclusions: ZnS-Au-GC was successfully prepared using an easy method. The results showed that the obtained material exhibited excellent performance in glycopeptide enrichment. Furthermore, it had showed great potential for glycopeptide enrichment in complex biological samples.

Easy fabrication of l-glutamic acid/ZnS composites for efficient photo-catalytic and supercapacitor performance

“…In a previous study, we observed that the absorbance maxima were changed very slowly for MB without the use of ZnS on sunlight illumination. 16 …”

“…In a previous study, we observed that the absorbance maxima were changed very slowly for MB without the use of ZnS on sunlight illumination. 16 Such data indicates the efficient photocatalytic ability of ZnS under sunlight exposure. Further, under the same experimental conditions, L-GA/ZnS composites were employed as photocatalysts to evaluate their photocatalytic performance with that of L-GA alone.…”

“…L-GA/ZnS composites were fabricated utilizing a facile solvent casting method. 16 In a similar fashion, ZnS composites were synthesized without the inclusion of L-GA. The typical procedure involves dissolving a known amount of L-GA (0.5 g) in 25 mL of double-distilled water and then heating the mixtures at 60 °C through continuous stirring for 1 hour until they become completely soluble.…”

“…Similar results were observed for other polymer composite materials. 16 Fig. 9 shows the percentage of removal MB for L-GA, ZnS, and all L-GA/ZnS composite samples as a function of irradiation time.…”