Hydrophilic carrageenan functionalized magnetic carbon‐based framework linked by silane coupling agent for the enrichment of N‐glycopeptides from human saliva

Abstract

In this work, a magnetic graphene material coated with mesoporous silica was selected as the substrate, 3-glycidoxypropyltrimethoxysilane and polyethyleneimine were sequentially bonded through chemical reactions, and then carrageenan was successfully introduced by electrostatic interaction; finally, hydrophilic nanocomposite material was prepared. Due to the large number of hydrophilic groups, and polyethyleneimine was connected by means of chemical bonds, this material exhibits good hydrophilicity and stability for glycopeptide enrichment. In the actual enrichment process, nanomaterial exhibits high selectivity (1:500), high sensitivity (2 fmol), and good repeatability (five cycles). In addition, the synthesized material also shows a good enrichment effect in the face of actual complex biological samples, which captured 40 Nglycopeptides from human saliva, indicating the application potential for enrichment of N-glycopeptides.

Bi‐amino acid functionalized biomimetic honeycomb chitosan membrane as a multifunctional hydrophilic probe for specific capture of N‐linked glycopeptides in nasopharyngeal carcinoma's disease patient's serum

“…As an important post-translational modification, protein glycosylation is involved in a range of biological processes [1], such as host-pathogen interaction and signal transduction [2][3][4], and abnormal glycosylation is usually significantly related to diseases like cancer [5][6][7]. Therefore, in-depth study of glycosylation will help to further understand the sites of action of glycosylation and find new disease-related biomarkers, providing the research direction with broad application prospects in the fields of clinical medicine and biochemistry [8].…”