Hydroperoxyde als Tr�ger der darmerregenden Wirkung hochunges�ttigter Fetts�uren

Dakhil, T.; Vogt, W.

doi:10.1007/bf00245107

Cited by 21 publications

(7 citation statements)

References 23 publications

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“…This technique has previously been used successfully as a supplement to other methods for identification of minute quantities of biologically active principles (cf. Ambache 1959, Dakhil andVogt 1962, Ramwell andShaw 1966). Judging from the results of the present study the purified smooth muscle stimulating principle is an unsaturated hydroxycarboxylic acid.…”

Section: Vmcular Effects In Guinea-pig Skinsupporting

confidence: 59%

Purification and Properties of the Slow Reacting Substance Formed in the Cat Paw Perfused with Compound 48/80¹

Strandberg

Uvnäs

1971

Acta Physiologica Scandinavica

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STRANDBERG, K. and B. UVNAS. Purification and flroperties of the slow reacting substance formed in the cat paw perfused with compound 48/80. Acta physiol. scand. 1971. 82. 358-374.Cat paws were perfused with a physiological salt solution containing compound 48/80. In addition to histamine, a principle producing a slow sustained contraction of the isolated guineapig ileum appeared in the effluent. This slow reacting substance (SRS) was purified by ethanol extraction, solvent partition, silicic acid and anion exchange chromatography. The overall recovery was 10-20 per cent, calculated from the non-histamine smooth muscle stimulating activity of the ethanolic extract. The purification calculated from the crude lipid extract was about 200-fold. The purified material was diaJysable, thermostable at neutral pH and more labile in acid than in alkaline milieu. I t behaved homogenously on thin layer chromatography in several systems. The most marked biological effects were contraction of the isolated guineapig ileum and the human bronchus, increases in the bronchial resistance in guinea-pigs in vivo, in the colouration (Evan's blue dye i.v.) of guinea-pig skin after intradermal injection and in the blood flow in the cat hindlimb after intraarterial injection. Treatment of the purified material with N,N'-carbo-di-p-tolylimide, phenyl isocyanate, iodine monobromide, potassium permanganate or acetic anhydride abolished or greatly reduced the biological activity. Incubation of SRS with 15-hydroxy prostaglandin dehydrogenase in the presence of NAD' resulted in no loss of biological activity. Together the results indicate that SRS is a biologically active carboxylic acid with hydroxyl groups and one or more double bonds but probably of nonprostaglandin nature.

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Section: Vmcular Effects In Guinea-pig Skinsupporting

confidence: 59%

Purification and Properties of the Slow Reacting Substance Formed in the Cat Paw Perfused with Compound 48/80¹

Strandberg

Uvnäs

1971

Acta Physiologica Scandinavica

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“…Dakhil & Vogt (1962) showed that some of the smooth muscle stimulating activity of unsaturated long-chain fatty acids was due to the formation of peroxides. RCS has strong pharmacological activity in that it contracts rabbit aorta (Piper & Vane, 1969) and many other arterial muscle strips from several species (Piper, unpublished).…”

Section: Discussionmentioning

confidence: 99%

“…The prostaglandin precursors, arachidonic acid and bis-homo-y-linolenic acid have several pharmacological activities (Horton & Main, 1966) although in general they are much weaker than the prostaglandins. Dakhil & Vogt (1962) showed that some of the smooth muscle stimulating activity of unsaturated long-chain fatty acids was due to the formation of peroxides. RCS has strong pharmacological activity in that it contracts rabbit aorta (Piper & Vane, 1969) and many other arterial muscle strips from several species (Piper, unpublished).…”

Section: Discussionmentioning

confidence: 99%

The release of prostaglandins and rabbit aorta contracting substance (RCS) from rabbit spleen and its antagonism by anti‐inflammatory drugs

Gryglewski

Vane

1972

British J Pharmacology

108

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Summary . Slices of rabbit spleen generate rabbit aorta contracting substance (RCS) and a prostaglandin of the E series when they are vibrated or stirred. . The release of both substances is increased by arachidonic acid and inhibited by aspirin‐like drugs. . When the spleen effluent containing RCS and prostaglandin E is incubated for a further 3 min, the prostaglandin concentration increases and the RCS concentration declines. . RCS may therefore be an unstable intermediate in the biosynthesis of prostaglandins.

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“…Aspirin in the doses used did not affect this inactivation. Since it is likely that the spasmogenic activity of arachidonic acid is due to peroxide formation in the solution (Dakhil & Vogt, 1962) it seems probable that the lungs can inactivate fatty acid peroxides.…”

Section: Discussionmentioning

confidence: 99%

Release of rabbit aorta contracting substance (RCS) and prostaglandins induced by chemical or mechanical stimulation of guinea‐pig lungs

Palmer

Piper

Vane

1973

British J Pharmacology

214

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Summary1. Rabbit aorta contracting substance (RCS) and prostaglandins were released from guinea-pig isolated perfused lungs by gentle massage and also by infusion of Prosparol.2. RCS and prostaglandins were also released by infusion into the pulmonary artery of bradykinin, arachidonic and dihomo-'y-linolenic acids or shock perfusate (containing RCS-releasing factor).3. Arachidonic and dihomo-y-linolenic acids caused a prolonged release of RCS and prostaglandins whereas bradykinin and shock perfusate gave a short-lasting output. 4. RCS and prostaglandins, together with histamine were released when superfused chopped lung tissue was agitated. 5. Challenge of sensitized guinea-pigs in vivo led to the release of an RCSlike substance into the carotid arterial blood. 6. Intravenous injection of bradykinin into guinea-pigs also released an RCSlike substance. 7. The release of RCS and prostaglandins was inhibited by aspirin or indomethacin in all experiments. 8. RCS contracted all vascular tissues investigated and also rat stomach strip. 9. The half-life of RCS was estimated as 1-2 minutes.

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Hydroperoxyde als Tr�ger der darmerregenden Wirkung hochunges�ttigter Fetts�uren

Cited by 21 publications

References 23 publications

Purification and Properties of the Slow Reacting Substance Formed in the Cat Paw Perfused with Compound 48/80¹

Purification and Properties of the Slow Reacting Substance Formed in the Cat Paw Perfused with Compound 48/80¹

The release of prostaglandins and rabbit aorta contracting substance (RCS) from rabbit spleen and its antagonism by anti‐inflammatory drugs

Release of rabbit aorta contracting substance (RCS) and prostaglandins induced by chemical or mechanical stimulation of guinea‐pig lungs

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Hydroperoxyde als Tr�ger der darmerregenden Wirkung hochunges�ttigter Fetts�uren

Cited by 21 publications

References 23 publications

Purification and Properties of the Slow Reacting Substance Formed in the Cat Paw Perfused with Compound 48/801

Purification and Properties of the Slow Reacting Substance Formed in the Cat Paw Perfused with Compound 48/801

The release of prostaglandins and rabbit aorta contracting substance (RCS) from rabbit spleen and its antagonism by anti‐inflammatory drugs

Release of rabbit aorta contracting substance (RCS) and prostaglandins induced by chemical or mechanical stimulation of guinea‐pig lungs

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Purification and Properties of the Slow Reacting Substance Formed in the Cat Paw Perfused with Compound 48/80¹

Purification and Properties of the Slow Reacting Substance Formed in the Cat Paw Perfused with Compound 48/80¹