Hydromechanical stress in shake flasks: Correlation for the maximum local energy dissipation rate

Peter, Cyril P.; Suzuki, Yusuke; Büchs, Jochen

doi:10.1002/bit.20827

Cited by 98 publications

(65 citation statements)

References 57 publications

(93 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is a further confirmation that the extreme and not the average values of e are relevant when considering processing suspension of containing dispersed particles. Similar conclusions were reported by other authors 83,84 when studying droplet breakup and found that the maximum stable droplet size scales with the maximum energy dissipation rate present in the system.…”

Section: Scaling Of Maximum Stable Aggregate Size With E Maxsupporting

confidence: 88%

Determination of maximum turbulent energy dissipation rate generated by a rushton impeller through large eddy simulation

et al. 2013

View full text Add to dashboard Cite

Large eddy simulation (LES) of a stirred tank equipped with a Rushton impeller and four cylindrical baffles was used to characterize the flow pattern and to assess the maximum turbulent kinetic energy dissipation rate e max . While the shorter baffle-impeller distance significantly affects the radial velocity profile and the trailing vortices expansion, the flow field in the impeller vicinity is comparable to that of a standard setup with rectangular baffles connected to the wall. The phase-resolved profile of e max indicates its very strong variation from 10 Á N 3 D 2 to 130 6 13 Á N 3 D 2. When using peak values of the corresponding hydrodynamic stress s max 5 ffiffiffiffiffiffiffiffiffiffiffiffiffiffi lqe max p À Á , the maximum stable aggregate size measured in the same stirred tank closely correlates with breakage data obtained under laminar conditions using the same initial aggregates. This indicates that the same mechanism was involved in the aggregate breakup under both conditions, allowing us to predict aggregates breakup under various conditions.

show abstract

Section: Scaling Of Maximum Stable Aggregate Size With E Maxsupporting

confidence: 88%

Determination of maximum turbulent energy dissipation rate generated by a rushton impeller through large eddy simulation

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Our measurements, however, in agreement with the other few previous direct measurements (Zirbel et al 2000, Kaku et al 2006, show a much steeper relationship. Thus, the 2.8 relationship, which has been empirically tested (Büchs et al 2000, Peter et al 2006), seems to hold only for the range of conditions usual in shaking bioreactors, in which the volume of fluid is <20% of the nominal flask volume (e.g., Büchs et al 2000), and the frequency of oscillation is usually much higher. Under these conditions, the kinetic energy production rate is very high, typically above 5 × 10 3 cm 2 s -3 , and the bottom of the flask runs dry (Büchs et al 2000).…”

Section: Fig 4 Turbulent Kinetic Energy Dissipation Rate (ε) Plottementioning

confidence: 99%

Evaluation of oscillating grids and orbital shakers as means to generate isotropic and homogeneous small‐scale turbulence in laboratory enclosures commonly used in plankton studies

Guadayol

Peters

Stiansen

et al. 2009

Limnology & Ocean Methods

View full text Add to dashboard Cite

The effects of turbulent motion on planktonic organisms have mainly been studied in the laboratory with devices capable of generating controlled turbulent conditions. Owing to technical and logistical difficulties, thorough assessments of hydrodynamics in such experiments are not routinely made. In this study, we examined the suitability of two widely used systems to generate isotropic, homogeneous, and stationary turbulence in laboratory containers: oscillating grid devices with large stroke length and relatively low frequencies of oscillation and orbital shaker tables. Turbulent kinetic energy dissipation rates were estimated from velocity measurements made with acoustic Doppler velocimeters. Both systems were shown to generate isotropic conditions in a relatively broad range of dissipation rates. Grid-stirred tanks produce homogeneous turbulence in both the horizontal and vertical dimensions, as long as stroke length is comparable to the height of the container. Turbulence in orbital shakers is not completely homogeneous, as it depends on the distance to the wall and to the surface. Empirical models are derived as a tool for the calculation of dissipation rates in the two systems within the ranges and conditions examined in this study.

show abstract

“…In recent years, the knowledge about the conditions in shake flasks has increased. (Maier, 2002;Maier and Büchs, 2001;Maier et al, 2004) characterized the mass-transfer (Büchs et al, 2000a,b;Peter et al, 2004) characterized the power input, Peter et al (2006) the hydromechanical stress, and (Maier and Büchs, 2001;Peter et al, 2004) the fluid movement. Until lately shake flask experiments could be performed only in the batch mode of operation.…”

Section: Introductionmentioning

confidence: 98%

Scale‐up from shake flasks to fermenters in batch and continuous mode with Corynebacterium glutamicum on lactic acid based on oxygen transfer and pH

Seletzky

Noak

Fricke

et al. 2007

Biotech & Bioengineering

Self Cite

View full text Add to dashboard Cite

Scale-up from shake flasks to fermenters has been hampered by the lack of knowledge concerning the influence of operating conditions on mass transfer, hydromechanics, and power input. However, in recent years the properties of shake flasks have been described with empirical models. A practical scale-up strategy for everyday use is introduced for the scale-up of aerobic cultures from shake flasks to fermenters in batch and continuous mode. The strategy is based on empirical correlations of the volumetric mass transfer coefficient (k(L) a) and the pH. The accuracy of the empirical k(L) a correlations and the assumptions required to use these correlations for an arbitrary biological medium are discussed. To determine the optimal pH of the culture medium a simple laboratory method based on titration curves of the medium and a mechanistic pH model, which is solely based on the medium composition, is applied. The effectiveness of the scale-up strategy is demonstrated by comparing the behavior of Corynebacterium glutamicum on lactic acid in shake flasks and fermenters in batch and continuous mode. The maximum growth rate (micro(max) = 0.32 h(-1)) and the oxygen substrate coefficient (Y O2 /S= 0.0174 mol/l) of C. glutamicum on lactic acid were equal for shake flask, fermenter, batch, and continuous cultures. The biomass substrate yield was independent of the scale, but was lower in batch cultures (Y(X/S) = 0.36 g/g) than in continuous cultures (Y(X/S) = 0.45 g/g). The experimental data (biomass, respiration, pH) could be described with a simple biological model combined with a mechanistic pH model.

show abstract

Hydromechanical stress in shake flasks: Correlation for the maximum local energy dissipation rate

Cited by 98 publications

References 57 publications

Determination of maximum turbulent energy dissipation rate generated by a rushton impeller through large eddy simulation

Determination of maximum turbulent energy dissipation rate generated by a rushton impeller through large eddy simulation

Evaluation of oscillating grids and orbital shakers as means to generate isotropic and homogeneous small‐scale turbulence in laboratory enclosures commonly used in plankton studies

Scale‐up from shake flasks to fermenters in batch and continuous mode with Corynebacterium glutamicum on lactic acid based on oxygen transfer and pH

Contact Info

Product

Resources

About