Hydrolyzed tilapia fish collagen induces osteogenic differentiation of human periodontal ligament cells

Abstract

Alveolar bone regeneration has aroused worldwide attention and plays an important role in oral clinics. In recent years, the application of biomaterials to induce osteogenic differentiation of periodontal ligament cells has become the hot topic in the field of alveolar bone regeneration. At present, most existing biomaterials lack osteoinductivity, while extrinsic inducers carry the risk of unwanted side effects. The objective of this work was to study the in vitro functionality of a newly developed hydrolyzed tilapia fish collagen (HFC) for periodontal tissue regeneration. HFC was extracted from the scales of tilapia, human periodontal ligament cells (hPDL cells) were cultured with HFC without the addition of any inducing reagent, and the effects of HFC on cell viability and osteogenic differentiation were investigated. The results revealed that HFC promoted the cell viability of hPDL cells. Furthermore, the upregulation of osteogenic markers ALP, COL I, RUNX2, and OCN at the gene level and the production of osteogenic-related proteins (alkaline phosphatase and osteocalcin) proved the success of osteogenic differentiation of hPDL cells treated with HFC. In addition, we revealed that the effect of HFC was mediated by ERK signaling pathways. Taken together, the data presented in this paper suggested for the first time that HFC is a promising bioactive ingredient for biomaterials used in alveolar bone regeneration.

Expression and regulation of the ERK1/2 and p38 MAPK signaling pathways in periodontal tissue remodeling of orthodontic tooth movement

“…It was suggested that flow shear stress stimulated osteogenic differentiation of hPDLCs via the ERK1/2 and p38 MAPK signaling pathways. Activation of the ERK1/2 pathway and upregulation of osteogenesis-associated genes may accelerate osteogenesis and calcification of hPDLCs ( 8 , 28 ). Therefore, in the present study, the effects of an ERK1/2 inhibitor and p38 MAPK inhibitor on osteogenesis-associated genes in hPDLCs were investigated.…”

“…Consequently, signals are transduced to the cell nucleus, regulating the endonuclear genes and stimulating a series of biological reactions. Various signaling pathways, including the mitogen-activated protein kinase (MAPK) pathway are involved in this biological process ( 6 – 8 ). The MAPK pathway is composed of a series of Ser/Thr kinases, including extracellular signal-regulated kinase (ERK)1/2, c-Jun N-terminal kinase (JNK), p38 and ERK5 subfamilies, which after cascade phosphorylation, regulate the activity of certain transcription factors.…”

Fish Collagen: Extraction, Characterization, and Applications for Biomaterials Engineering

“…In vivo studies under growth factor-free conditions showed a favorable interaction between the collagen sample and the surrounding tissue. Liu et al [ 134 ] investigated the in vitro functionality of tilapia fish collagen for periodontal tissue regeneration. The results of cultured human periodontal ligament cells with hydrolyzed fish collagen were investigated by using osteogenic markers ALP, COL I, RUNX2, and OCN at the gene level, and the production of osteogenic-related proteins revealed favorable cell viability and osteogenic differentiation.…”

Comparative proteomics analysis of teleost intermuscular bones and ribs provides insight into their development

“…Osteocalcin and annexins A2a protein related with osteoblasts were identified as down-regulated proteins in both 1-IB-vs-1-Rib and 2-IB-vs-2-Rib groups, which mean they had higher expression in both 1- and 2-year old ribs compared with that of IBs. It had been reported that osteocalcin could induce bone regeneration and have distinct roles in the dentin mineralization process [ 38 , 39 ], so it may play a critical role in the development and growth of ribs. Due to localization of annexins in areas of cartilage and bone mineralization, annexins made an important role in mineralization process.…”