Hydrolyzed Ce(IV) salts limit sucrose-dependent biofilm formation by Streptococcus mutans

Bhatt, Lopa; Chen, Lin; Guo, Jinglong; Klie, Robert F.; Shi, Junhe; Pesavento, Russell P.

doi:10.1016/j.jinorgbio.2020.110997

Cited by 8 publications

(11 citation statements)

References 31 publications

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“…Streptococcus mutans forms biofilms that contribute to dental caries in the presence of fermentable carbohydrates and constitutes a target for therapeutic intervention. Ceria NPs reduced bacterial adhesion by 40%, and planktonic growth and dispersal assays supported a non-bactericidal mode of biofilm inhibition [ 188 ]. A detailed study of the bio–nano interface demonstrated that rod-like ceria NPs could be reduced by Bacillus subtilis under planktonic conditions, so that the Ce (III) ions adjacent to the surface oxygen vacancies would be chelated by the adsorption sites present on the bacterial cell wall.…”

Section: (Bio)applicationsmentioning

confidence: 99%

Nanostructured Ceria: Biomolecular Templates and (Bio)applications

et al. 2021

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Ceria (CeO2) nanostructures are well-known in catalysis for energy and environmental preservation and remediation. Recently, they have also been gaining momentum for biological applications in virtue of their unique redox properties that make them antioxidant or pro-oxidant, depending on the experimental conditions and ceria nanomorphology. In particular, interest has grown in the use of biotemplates to exert control over ceria morphology and reactivity. However, only a handful of reports exist on the use of specific biomolecules to template ceria nucleation and growth into defined nanostructures. This review focusses on the latest advancements in the area of biomolecular templates for ceria nanostructures and existing opportunities for their (bio)applications.

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Section: (Bio)applicationsmentioning

confidence: 99%

Nanostructured Ceria: Biomolecular Templates and (Bio)applications

et al. 2021

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“…, nanoceria, CeO 2 -NP, 3–5 nm) prepared from the in situ hydrolysis of ceric nitrate limited in vitro biofilm adherence of S. mutans with limited toxicity to either human or bacterial cells at moderate concentrations . We continued to investigate these nanoceria as potential oral antibiofilm agents due to their potential for reduced harmful effects on the microbiota.…”

Section: Introductionmentioning

confidence: 99%

Nanoceria Aggregate Formulation Promotes Buffer Stability, Cell Clustering, and Reduction of Adherent Biofilm in Streptococcus mutans

Ellepola,

Bhatt,

Chen

et al. 2023

ACS Biomater. Sci. Eng.

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Streptococcus mutans is one of the key etiological factors in tooth-borne biofilm development that leads to dental caries in the presence of fermentable sugars. We previously reported on the ability of acid-stabilized nanoceria (CeO2-NP) produced by the hydrolysis of ceric salts to limit biofilm adherence of S. mutans via non-bactericidal mechanism(s). Herein, we report a chondroitin sulfate A (CSA) formulation (CeO2-NP-CSA) comprising nanoceria aggregates that promotes resistance to bulk precipitation under a range of conditions with retention of the biofilm-inhibiting activity, allowing for a more thorough mechanistic study of its bioactivity. The principal mechanism of reduced in vitro biofilm adherence of S. mutans by CeO2-NP-CSA is the production of nonadherent cell clusters. Additionally, dose-dependent in vitro human cell toxicity studies demonstrated no additional toxicity beyond that of equimolar doses of sodium fluoride, currently utilized in many oral health products. This study represents a unique approach and use of a nanoceria aggregate formulation with implications for promoting oral health and dental caries prevention as an adjunctive treatment.

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“…However, nanoparticles often cannot endure rigorous surface treatments to recondition surfaces after eventual biofilm formation and/or fouling. Metal salts (e.g., Ce(IV)) have been shown to disrupt saccharide-dependent biofilm formation during the kinetically controlled reversible adhesion of primary colonization ( Bhatt et al., 2020 ). However, metal salts often do not persist long term in aqueous environments thereby rendering them inactive.…”

Section: Introductionmentioning

confidence: 99%

Thermodynamic Surface Analyses to Inform Biofilm Resistance

et al. 2020

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Biofilms are the habitat of 95% of bacteria successfully protecting bacteria from many antibiotics. However, inhibiting biofilm formation is difficult in that it is a complex system involving the physical and chemical interaction of both substrate and bacteria. Focusing on the substrate surface and potential interactions with bacteria, we examined both physical and chemical properties of substrates coated with a series of phenyl acrylate monomer derivatives. Atomic force microscopy (AFM) showed smooth surfaces often approximating surgical grade steel. Induced biofilm growth of five separate bacteria on copolymer samples comprising varying concentrations of phenyl acrylate monomer derivatives evidenced differing degrees of biofilm resistance via optical microscopy. Using goniometric surface analyses, the van Oss-Chaudhury-Good equation was solved linear algebraically to determine the surface energy profile of each polymerized phenyl acrylate monomer derivative, two bacteria, and collagen. Based on the microscopy and surface energy profiles, a thermodynamic explanation for biofilm resistance is posited.

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Hydrolyzed Ce(IV) salts limit sucrose-dependent biofilm formation by Streptococcus mutans

Cited by 8 publications

References 31 publications

Nanostructured Ceria: Biomolecular Templates and (Bio)applications

Nanostructured Ceria: Biomolecular Templates and (Bio)applications

Nanoceria Aggregate Formulation Promotes Buffer Stability, Cell Clustering, and Reduction of Adherent Biofilm in Streptococcus mutans

Thermodynamic Surface Analyses to Inform Biofilm Resistance

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