Hydrolytic Degradation of Poly(3-hydroxybutyrate), Polylactide and their Derivatives: Kinetics, Crystallinity, and Surface Morphology

Бонарцев, А. П.; Boskhomodgiev, A. P.; Иорданский, А. Л.; Бонарцева, Г. А.; Ребров, А. В.; Махина, Т. К.; Мышкина, В. Л.; Yakovlev, S. A.; Филатова, Е. В.; Иванов, Е. А.; Bagrov, D. V.; Заиков, Г. Е.

doi:10.1080/15421406.2012.635982

Cited by 78 publications

(57 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The introduction of 3HV into the macromolecule enhanced the hydrolytic stability of the copolymer. The enhanced hydrolytic stability of the PHBV copolymers compared to PHB copolymers corresponded with the literature and may be explained by the higher hydrophobicity of PHBV than PHB [46]. The decrease of molecular weight in time for all PHA scaffolds showed typical exponential decay.…”

Section: Discussionsupporting

confidence: 81%

Enzymatic Hydrolysis of Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) Scaffolds

et al. 2020

View full text Add to dashboard Cite

Polyhydroxyalkanoates (PHAs) are hydrolyzable bio-polyesters. The possibility of utilizing lignocellulosic waste by-products and grape pomace as carbon sources for PHA biosynthesis was investigated. PHAs were biosynthesized by employing Cupriavidus necator grown on fructose (PHBV-1) or grape sugar extract (PHBV-2). Fifty grams of lyophilized grape sugar extract contained 19.2 g of glucose, 19.1 g of fructose, 2.7 g of pectin, 0.52 g of polyphenols, 0.51 g of flavonoids and 7.97 g of non-identified rest compounds. The grape sugar extract supported the higher production of biomass and modified the composition of PHBV-2. The biosynthesized PHAs served as matrices for the preparation of the scaffolds. The PHBV-2 scaffolds had about 44.2% lower crystallinity compared to the PHBV-1 scaffolds. The degree of crystallinity markedly influenced the mechanical behavior and enzymatic hydrolysis of the PHA scaffolds in the synthetic gastric juice and phosphate buffer saline solution with the lipase for 81 days. The higher proportion of amorphous moieties in PHBV-2 accelerated enzymatic hydrolysis. After 81-days of lasting enzymatic hydrolysis, the morphological changes of the PHBV-1 scaffolds were negligible compared to the visible destruction of the PHBV-2 scaffolds. These results indicated that the presence of pectin and phenolic moieties in PHBV may markedly change the semi-crystalline character of PHBV, as well as its mechanical properties and the course of abiotic or enzymatic hydrolysis.

show abstract

Section: Discussionsupporting

confidence: 81%

Enzymatic Hydrolysis of Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) Scaffolds

et al. 2020

View full text Add to dashboard Cite

show abstract

“…In addition, temperature also affects the state of a polymer, which in turn will cause non-enzymatic hydrolytic reactions. Previous report showed that higher temperature would decrease the molecular weight of PHA (Bonartsev et al 2012 ). Therefore, the effect of temperature on polymer films and lipase depolymerizing activities was studied.…”

Section: Discussionmentioning

confidence: 95%

Characterization of the depolymerizing activity of commercial lipases and detection of lipase-like activities in animal organ extracts using poly(3-hydroxybutyrate-co-4-hydroxybutyrate) thin film

et al. 2016

View full text Add to dashboard Cite

Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] is one of the polyhydroxyalkanoate (PHA) copolymers which can be degraded by lipases. In this study, the depolymerizing activity of different known commercial lipases was investigated via microassay using P(3HB-co-92 mol % 4HB) thin film as substrate. Non-enzymatic hydrolysis occurred under conditions in which buffers with pH 12 and 13 were added or temperature of 50 °C and above. Different concentrations of metal ions or detergents alone did not cause the film hydrolysis. The depolymerizing activity of lipases on P(3HB-co-4HB) was optimum in the pH range of 6–8 and at temperatures between 30 and 50 °C. Addition of metal ions and detergents in different concentrations was also shown to cause variable effects on the depolymerizing activity of commercial lipases. Pancreatic extracts from both mouse and chicken showed similar depolymerizing activity as the commercial lipases on the P(3HB-co-4HB) film. The presence of lipolytic enzymes in the organ extracts was confirmed with another lipase activity assay, p-nitrophenyl laurate assay. For the first time this has produced a direct evidence for the involvement of lipase-like enzymes from animal in the degradation of this PHA. Lipase is most likely the enzyme from pancreas that was involved in the degradation.Electronic supplementary materialThe online version of this article (doi:10.1186/s13568-016-0230-z) contains supplementary material, which is available to authorized users.

show abstract

“…PHB is prepared by a biotechnology route, possesses promising properties such as a high level of biocompatibility [7][8][9], and can undergo biodegradation in living tissues without forming toxic substances [10][11][12]. Therefore, PHB is used in the development of medical implements for surgery, dentistry, cardiac surgery, orthopedics, and other areas [13,14].…”

Section: Supramolecular Structure Formation Of Poly(3-hydroxybutyratementioning

confidence: 99%

Structure-Formation Features in Ultrathin Fibers of Poly(3-Hydroxybutyrate) Modified with Nanoparticles

et al. 2016

Self Cite

View full text Add to dashboard Cite

Supramolecular structure formation of poly(3-hydroxybutyrate) ultrathin fibers prepared by electrospinning and the influence of low concentrations of silicon andTiO 2 nanoparticles on the structure, physicomechanical and sorption properties, and resistance to thermal destruction and thermoand photo-oxidative destruction of non-woven fibrous material based on these fibers were studied. It was found that nanoparticles promoted the formation of thinner fibers with enhanced physicomechanical parameters and a structure that was more resistant to thermal and thermo-and photo-oxidative destruction and had a positive effect on the growth dynamics of mesenchymal stem cells.Tissue engineering utilizes various polymeric constructs prepared by one of the most promising methods, i.e., electrospinning (ES) of fibrous nanocomposites from biopolymers [1-3]. The method allows fibrous scaffolds with large surface-to-volume ratios to be produced. This enables free migration and proliferation of cells into the three-dimensional matrix and; therefore, excellent integration of the material into living tissues [4].Polyoxyalkanoates (POA), the most scrutinized of which is poly(3-hydroxybutyrate) (PHB), are special biopolymers [5,6]. PHB is prepared by a biotechnology route, possesses promising properties such as a high level of biocompatibility [7][8][9], and can undergo biodegradation in living tissues without forming toxic substances [10][11][12]. Therefore, PHB is used in the development of medical implements for surgery, dentistry, cardiac surgery, orthopedics, and other areas [13,14].The diameter of a monofilament and its molecular and supramolecular structure affect its physicomechanical and diffusion properties, the kinetics of oxidative and photo-oxidative reactions, and the biodegradation of fibrous non-woven materials [7]. The diameter of fibers produced by ES depends on parameters such as the concentration of the substance in the starting solution, solvent evaporation rate, potential, distance from the needle to the spun layer, electrical conductivity, viscosity, temperature, etc.[4]. Therefore, the production of a finished fiber with a definite morphology continues to be a critical problem.However, the diameter of PHB ultrathin fibers is practically unaffected by changing the ES parameters (electrical conductivity, throughput, spinning solution viscosity) [15,16]. Therefore, the structure and properties of non-woven PHB materials remain practically unchanged.The creation of modern bioresorbable fibrous materials for use as matrices for targeted drug delivery, scaffolds for growing living cells, artificial ligaments, etc. requires a method for varying the PHB fiber structural parameters in order to construct materials with the desired array of properties.

show abstract

Hydrolytic Degradation of Poly(3-hydroxybutyrate), Polylactide and their Derivatives: Kinetics, Crystallinity, and Surface Morphology

Cited by 78 publications

References 25 publications

Enzymatic Hydrolysis of Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) Scaffolds

Enzymatic Hydrolysis of Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) Scaffolds

Characterization of the depolymerizing activity of commercial lipases and detection of lipase-like activities in animal organ extracts using poly(3-hydroxybutyrate-co-4-hydroxybutyrate) thin film

Structure-Formation Features in Ultrathin Fibers of Poly(3-Hydroxybutyrate) Modified with Nanoparticles

Contact Info

Product

Resources

About