Hydrolytic and transphosphatidylation activities of phospholipase D from Savoy cabbage towards lysophosphatidylcholine

A Short Review on Various Screening Methods to Isolate Potential Lipase Producers: Lipases-the Present and Future Enzymes of Biotech Industry

“…Quantification of individual phospholipid compounds was performed by HPTLC in studies by More and Pandit (2010). The method was also used by Vitro et al (2000) for determining the fatty acid distribution. Rajan et al (2011) quantified the liberated free fatty acids produced by the action of alkaline lipase from Aspergillus fumigates MTCC 9657 using HPTLC.…”

Phospholipase D and its application in biocatalysis

“…This method, which is based on the binding of PLD onto octyl-Sepharose in the presence of Ca 2+ and the subsequent selective elution of the enzyme by EDTA, has successfully been used for the purification of PLD from plant leaves or seedlings as well as from E. coli in recombinant PLD production (Novotna´et al 1999, Virto et al 2000, Oblozinsky et al 2003. Interestingly, Ca 2+ concentration necessary for binding of PLD to the hydrophobic carrier correlates with the Ca 2+ concentration of maximum activation.…”

Membrane lipids and cell death: an overview

“…PLD activation normally follows receptor-mediated enhancement in PLC activity, the DAG product of which can indirectly activate PLD via the PKC pathway (Frohman et al, 1999;Nozawa, 2002). Moreover, PLD can attenuate signal transduction via its peculiar transphosphatidylation reaction condensing DAG with PC or LPC, instead of producing phosphatidic acid (PA), which is the major product of its hydrolytic activity (van Blitterswijk and Hilkmann, 1993;Sung et al, 1997;Virto et al, 2000).…”