Hydrolytic activity of a dinuclear copper(II,II) complex in phosphate diester and DNA cleavage

Rossi, Liane M.; Neves, Ademir; Hörner, Rosmari; Terenzi, Hernán; Szpoganicz, Bruno; Sugai, Juliet Kiyoko

doi:10.1016/s0020-1693(02)01111-8

Cited by 77 publications

(45 citation statements)

References 13 publications

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“…DNA cleavage was performed as described elsewhere [54,55]. The reaction was performed in a final volume of 20 µl containing 1 µg pBSK II plasmid DNA, 160 µM 1 and 25 mM 3-(Nmorpholino)propanesulfonic acid (MOPS), pH 7.0.…”

Section: Dna Cleavagementioning

confidence: 99%

A new heterobinuclear FeIIICuII complex with a single terminal FeIII–O(phenolate) bond. Relevance to purple acid phosphatases and nucleases

et al. 2005

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Section: Dna Cleavagementioning

confidence: 99%

A new heterobinuclear FeIIICuII complex with a single terminal FeIII–O(phenolate) bond. Relevance to purple acid phosphatases and nucleases

et al. 2005

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“…The design and synthesis of dinuclear 3d metal complexes is an attractive topic because of the potential applications of these compounds as catalysts, oxidizing agents, [1][2][3] electrochemical catalysts for the oxidation of alcohol, ether, and water, [4][5][6] biocatalysts for the hydrolysis of phosphodiesters, [7][8][9][10][11][12] and for DNA and RNA cleavage [12][13][14][15] as well as to mimic the active sites in metalloproteins. [16][17][18] Dinuclear dioxido-bridged complexes of Mn, Fe, Cu, and Ni have been reported to activate C-H bonds in aliphatic and aro-geometry around each copper center is achieved by two terminal chloro ligands.…”

Section: Introductionmentioning

confidence: 99%

“…[14,17,18,21,23,27,28] As alluded to above, one of the important properties of dinuclear metal(II) complexes is their increased efficiency in hydrolyzing phosphate esters and in cleavage reactions of DNA compared to the related mononuclear species. [12][13][14][15] Such bimetallic cooperativity has been observed in the hydrolysis of phosphate esters by dinuclear copper(II) and zinc(II) complexes. [12][13][14][15][29][30][31][32] For example, in the hydrolysis of phosphate monoester, a 50-fold enhancement in the rate of reaction was observed when two bis(benzimidazolyl)cop-per(II) units linked by a bridging 2-phenoxy-1,3-xylyl group were used compared to the corresponding monomer.…”

Section: Introductionmentioning

confidence: 99%

DNA Cleavage by Structurally Characterized Dinuclear Copper(II) Complexes Based on Triazine

Massoud

Louka

et al. 2011

Eur J Inorg Chem

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The reaction of 2‐chloro‐4,6‐bis(di‐2‐picolylamino)‐1,3,5‐triazine (bdpaTCl) with copper(II) perchlorate and copper(II) chloride afforded two dinuclear complexes [Cu2(μ‐bdpaTCl)(μ‐OH)2(H2O)0.5(ClO4)0.5](ClO4)1.5·(H2O)1.5 (1) and [Cu2(μ‐bdpaTCl)Cl4]·2CH3OH (2), respectively. These complexes were characterized by IR, UV/Vis, and EPR spectroscopy, single‐crystal X‐ray crystallography, and temperature dependence magnetic susceptibility measurements (2–300 K) as well as by electrochemical and molar conductivity measurements. In 1, each of the three N‐donor atoms of the binucleating bdpaTCl ligand coordinate to CuII ions, which are further bridged by two OH– anions in a distorted five‐coordinate geometry. In addition, each CuII ion forms a Cu–O semicoordinate bond with an aqua ligand or perchlorato anion. The Cu···Cu distance across the hydroxido bridges is 2.9698(11) Å. In 2, the bdpaTCl ligand acts as bis‐tridentate ligand connecting the two CuII ions, and the five‐coordinate geometry around each copper center is achieved by two terminal chloro ligands. Magnetic measurements revealed strong antiferromagnetic coupling in 1 (J = –311.2 cm–1) and very weak coupling in 2 (J = –2.4 cm–1). DNA cleavage by these two complexes has been investigated (pH = 7.0, 37 °C). Although the bridged dihydroxido complex 1 did not show any detectable cleavage for DNA, significant cleavage was observed with the tetrachloro complex 2. Under pseudo‐Michaelis–Menten kinetic conditions, the kinetic parameters kcat = 2.53 × 10–5 s–1 and KM = 1.44 × 10–4 M were determined for 2. The kcat value corresponds to a 2.5 × 106 fold rate enhancement over noncatalyzed DNA. Electrophoretic experiments conducted in the presence and absence of oxidative scavengers DMSO, KI, and NaN3, and radical promoter H2O2 provide evidence for the oxidative cleavage of DNA by hydroxy radicals and hydrogen peroxide species.

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“…Many mimetic models of hydrolase have been widely applied to the study of the ester hydrolysis, such as hydrolysis of carboxylic acid esters, [5][6][7] amino acid esters 8,9 and phosphoric acid esters. [10][11][12] The hydrolysis of carboxylic esters is of particular interests and paramount importance in biological and industrial processes. 13 In biological enzymes, zinc ion has been often found in the active sites, where it can activate the substrate and the water molecules.…”

Section: Introductionmentioning

confidence: 99%

Catalytic Kinetics of the Schiff Base Metal Complexes Bearing Side Chain of Cyclic morpholine in Carboxylic Ester Hydrolysis

Zhang¹,

Li²,

Ou³

et al. 2007

Chin. J. Chem.

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It has been reported that two Schiff base transition metal complexes bearing the side chain of the morpholine ring were synthesized and characterized, and two complexes with the same base agent but different metal ions were used as a simulant hydrolase in the catalytic hydrolysis of p-nitrophenyl picolinate in this paper. The mechanism of PNPP catalytic hydrolysis is proposed and supported by the results of the spectral analysis and the kinetic calculation. A kinetic mathematical model, applied to the calculation of the kinetic and thermodynamics parameters of PNPP catalytic hydrolysis, has been established on the foundation of the mechanism proposed. The result of the study shows that the two complexes have a good catalytic activity in PNPP catalytic hydrolysis, and the rate of the PNPP catalytic hydrolysis was increased with the increase of the pH values in the buffer solution and affected by the polarization effect of metal ion of the complexes.

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Hydrolytic activity of a dinuclear copper(II,II) complex in phosphate diester and DNA cleavage

Cited by 77 publications

References 13 publications

A new heterobinuclear FeIIICuII complex with a single terminal FeIII–O(phenolate) bond. Relevance to purple acid phosphatases and nucleases

A new heterobinuclear FeIIICuII complex with a single terminal FeIII–O(phenolate) bond. Relevance to purple acid phosphatases and nucleases

DNA Cleavage by Structurally Characterized Dinuclear Copper(II) Complexes Based on Triazine

Catalytic Kinetics of the Schiff Base Metal Complexes Bearing Side Chain of Cyclic morpholine in Carboxylic Ester Hydrolysis

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