Hydrolysis rates of olive oil by lipase in a monodispersed emulsion system using membrane emulsification

Shiomori, Koichiro; Hayashi, Toshimitsu; Baba, Yoshihiro; Kawano, Yoshinobu; Hano, Tadashi

doi:10.1016/0922-338x(96)87730-0

Cited by 26 publications

(16 citation statements)

References 16 publications

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“…In addition, it seems that the presence of SDS did not negatively affect the lipase activity in an emulsion environment. As reported, the cooperative function of PVA and SDS is favorable to effective hydrolysis by lipase in an emulsion system (Shiomori et al, 1995). The conversion as a function of time observed in the emulsion feed solution and in the permeate solution is reported in Figure 2 (the conversion in the permeate was calculated as the overall conversion minus the conversion observed in the emulsion feed).…”

Section: Performance Of Emulsion Enzyme Membrane Reactor Operating Inmentioning

confidence: 74%

Use of stable emulsion to improve stability, activity, and enantioselectivity of lipase immobilized in a membrane reactor

Giorno

Drioli

2003

Biotech & Bioengineering

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The enantiocatalytic performance of immobilized lipase in an emulsion membrane reactor using stable emulsion prepared by membrane emulsification technology was studied. The production of optical pure (S)-naproxen from racemic naproxen methyl ester was used as a model reaction system. The O/W emulsion, containing the substrate in the organic phase, was fed to the enzyme membrane reactor from shell-to-lumen. The enzyme was immobilized in the sponge layer (shell side) of capillary polyamide membrane with 50 kDa cut-off. The aqueous phase was able to permeate through the membrane while the microemulsion was retained by the thin selective layer. Therefore, the substrate was kept in the enzyme-loaded membrane while the water-soluble product was continuously removed from the reaction site. The results show that lipase maintained stable activity during the entire operation time (more than 250 h), showing an enantiomeric excess (96 +/- 2%) comparable to the free enzyme (98 +/- 1%) and much higher compared to similar lipase-loaded membrane reactors used in two-separate phase systems (90%). The results demonstrate that immobilized enzymes can achieve high stability as well as high catalytic activity and enantioselectivity.

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Section: Performance Of Emulsion Enzyme Membrane Reactor Operating Inmentioning

confidence: 74%

Use of stable emulsion to improve stability, activity, and enantioselectivity of lipase immobilized in a membrane reactor

Giorno

Drioli

2003

Biotech & Bioengineering

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“…Their transformation provides products with high market values such as monoglycerides, widely used as emulsifiers in the food, cosmetics and pharmaceutical industries (Schmid, 1987;Ranalli and Mattia, 1997;Shiomori et al, 1995;Bhaskar et al, 1993). Furthermore, the transesterification of oil and fats produces a mixture of esters known as biodiesel.…”

Section: Introductionmentioning

confidence: 99%

Phase behavior of olive and soybean oils in compressed propane and n-butane

Ndiaye

Lanza²,

Tavares

et al. 2006

Braz. J. Chem. Eng.

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-The aim of this work is to report the experimental data and thermodynamic modeling of phase equilibrium of binary systems containing soybean and olive oils with propane and n-butane. Phase equilibrium experiments were carried out using the static synthetic method in a high-pressure variable-volume view cell in the temperature range from 30 to 70 o C and varying the solvent overall composition from 5 to 98 wt%. Vapor-liquid, liquid-liquid and vapor-liquid-liquid phase transitions were observed at relatively low pressures. The Peng-Robinson and the SAFT equations of state without any binary interaction parameters were employed in an attempt at representing the phase behavior of the systems. Results show the satisfactory performance of SAFT-EoS in predicting qualitatively all phase transitions reported in this work.

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“…Several examples exist in the literature that use enzymes in organic media [37,38] but availability and cost are factors in choosing a workable system for this work. One enzyme of particular interest whose use is underway in our lab is the CandidaAntarctica lipase.…”

Section: Hydrolysis Of Polymersmentioning

confidence: 99%

Polyethylene Prodrugs Using Precisely Placed Pharmaceutical Agents

Leonard

Turek

Sloan

et al. 2010

Macro Chemistry & Physics

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Polyethylene‐based polymeric prodrugs have been prepared by acyclic diene metathesis (ADMET) polymerization; this condensation type, step growth polymerization, affords controlled polymer architectures via exact methylene run lengths between drug branches. Polyethylene was synthesized with the nonsteroidal anti‐inflammatory drug (NSAID) branches ibuprofen and naproxen attached at every 21st backbone carbon through a hydrolysable ester linkage. These ester linkages can have their reactivity tuned by using a variety of spacers that link the drug to the polymer backbone. Two types of spacers, tetraethylene glycol (TEG, hydrophilic) and decanediol (hydrophobic), were incorporated between the drugs and the polymer backbone to observe the effect of spacer properties on the rate of drug release. The rate of hydrolysis and subsequent release of drug was monitored as a direct effect of the spacer and type of reactive linker. With the primary structure of the polymers perfectly known, making such subtle changes to these structures has a dramatic influence on their physical properties. The polymers discussed herein are characterized with NMR, IR, TGA, and DSC. The rate at which these materials hydrolyze and release their pharmaceutical species via enzymatic or chemical hydrolysis was monitored by UV‐vis. Tailoring the type of spacer and hydrolysable linker to a particular drug offers a new class of polyethylene with controllable features that can be used in the application of a new drug delivery material.magnified image

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Hydrolysis rates of olive oil by lipase in a monodispersed emulsion system using membrane emulsification

Cited by 26 publications

References 16 publications

Use of stable emulsion to improve stability, activity, and enantioselectivity of lipase immobilized in a membrane reactor

Use of stable emulsion to improve stability, activity, and enantioselectivity of lipase immobilized in a membrane reactor

Phase behavior of olive and soybean oils in compressed propane and n-butane

Polyethylene Prodrugs Using Precisely Placed Pharmaceutical Agents

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