Hydrolysis of sucrose by invertase entrapped in polyvinyl alcohol hydrogel capsules

Rebroš, Martin; Rosenberg, Michal; Mlichová, Zuzana; Krištofíková, Ľ.

doi:10.1016/j.foodchem.2006.06.020

Cited by 63 publications

(55 citation statements)

References 15 publications

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“…The non-constant trend of activity during repeated bioconversions can be caused by different residual reaction components after each washing of particles. There were observed similar effect in work with PVA particles before [19]. The activity of the 90-min reaction dropped at the same speed as the others despite particles being exposed to the highest product concentrations, therefore inhibition was excluded.…”

Section: Repeated Biotransformationsmentioning

confidence: 53%

“…Storage at 4 • C caused a significant decrease in relative activity after 90 h ( Figure 4) and is therefore not suitable for these co-immobilized enzymes. Storage at 4 • C was chosen based on the previously reported application for immobilized invertase [19]. The particles retained 78% of activity after eight months.…”

Section: Storage Of Particlesmentioning

confidence: 99%

“…LentiKats ® is an entrapment technology that has been applied for the immobilization of whole cells and enzymes [13][14][15][16][17][18][19][20][21]. Its lens-shaped capsules are made of polyvinyl alcohol gel which offers several advantages such as low matrix costs, inexpensive and simple gel preparation, low diffusion limits, etc.…”

Section: Introductionmentioning

confidence: 99%

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Co-Immobilization of Ketoreductase and Glucose Dehydrogenase

et al. 2018

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Abstract:A two-enzyme system composed of immobilized ketoreductase (Hansenula polymorpha) and glucose dehydrogenase (Bacillus megaterium) was developed for the asymmetric reduction of keto esters to optically active hydroxy esters via immobilization in polyvinyl alcohol (PVA) gel particles. The concentration of enzymes was optimized, and the final particles were used 18 times in a row in a batch mode to achieve minimal loss of activity and complete conversion of the model substrate, β-ketoester ethyl-2-methylacetoacetate. Excellent stability was also achieved using new storage conditions of PVA particles, with 80% of activity being retained after almost 10 months.

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Section: Repeated Biotransformationsmentioning

confidence: 53%

Section: Storage Of Particlesmentioning

confidence: 99%

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Co-Immobilization of Ketoreductase and Glucose Dehydrogenase

et al. 2018

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“…The most crucial advantage of this method is being simple: It is a single-step technique providing mono-size particles under desired conditions (20). The polymeric magnetic particles are quite available candidates in terms of and due to the high mechanical resistance, insolubility and wonderful shelf-life (21), having a variety of surface functional groups, (22)(23)(24)(25)(26), good mechanical features, easy preparation, and biocompatibility developed for bio-friendly components (27). Poly(2-hydroxyethyl methacrylate) [Poly(HEMA)] has been considered as a suitable matrix for enzyme immobilization due to being a biocompatible synthetic polymer with a good mechanical strength for several biomedical and biotechnological applications (28).…”

Section: Introductionmentioning

confidence: 99%

Characterization of Magnetic Polymeric Microparticles

Köse¹

2016

J. Turk. Chem. Soc., Sect. A: Chem.

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Abstract:The separation process is very important in a variety of scientific fields, especially in biochemistry and biotechnology. The separation performance of a method can be determined by the separation time and the purity of the separated molecule, which is directly proportional with the ligand chosen for the target molecule. The separation time is actually a very important step for the cost and the time-dependent analysis, especially in medical applications. Magnetic separation is a very advantageous technique because it eliminates the time-consuming sample preparation and centrifugation steps. However, the magnetic performance of adsorbent may not be strong enough to respond the magnetic force applied externally. Therefore, in nanoparticle studies, magnetic performance of nanoparticles is highly important. In this study, the magnetic performance of polymeric microparticles synthesized using different magnetic cores (Fe2O3 & Fe3O4, Fe & Ni, Ni & Co, Fe & Ni & Co) with a solid support, HEMA (2-hydroxyethylmethacrylate), and a functional monomer, AdeM (adenine methacrylate), were compared. Some other properties such as size, zeta potential, surface morphology, etc. were also studied.

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“…[9][10][11][12][13] Polymeric materials are suitable candidates due to their reactive functional groups, good mechanical properties, ease of preparation method and applicability to introduce bio-friendly components for improving biocompatibility. 14 Polymeric gels have applications in many different areas of biotechnology. 15 One of the new types of polymer gels with considerable potential in biotechnology is 'cryogels'.…”

Section: Introductionmentioning

confidence: 99%

Cadmium Removal Performances of Different Dye Ligands Attached Cryogel Disks

Keleş¹,

Inanan²,

Tüzmen³

et al. 2015

Croat. Chem. Acta

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Abstract. Poly(HEMA) cryogel disks were synthesized by free radical polymerization of 2-hydroxyethylmethacrylate (HEMA), and then Cibacron Blue F3GA (CB), Reactive Green 19 (RG) and Congo Red (CR) were immobilized as dye ligands. Disks were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) and swelling degree, porosity calculations. Cd 2+ adsorption experiments were performed for 60 min. Maximum adsorption capacities were determined as 25.5 mg/g; 48.0 mg/g and 28.5 mg/g at pH = 7.0 for poly(HEMA)-CB; poly(HEMA)-RG and poly-(HEMA)-CR, respectively. Langmuir isotherm fitted best with the adsorption data and adsorption thermodynamics showed that Cd 2+ adsorption is thermodynamically favorable and a physisorption process. A great majority of adsorbed Cd 2+ desorbed with 1 M NaCl and cryogel disks can be re-used in adsorption experiments. Cd 2+ removal efficiencies of disks from human plasma are approximately 45 %. Dyeattached cryogel disks synthesized in this study have potential in use for environmental and therapeutic applications.

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Hydrolysis of sucrose by invertase entrapped in polyvinyl alcohol hydrogel capsules

Cited by 63 publications

References 15 publications

Co-Immobilization of Ketoreductase and Glucose Dehydrogenase

Co-Immobilization of Ketoreductase and Glucose Dehydrogenase

Characterization of Magnetic Polymeric Microparticles

Cadmium Removal Performances of Different Dye Ligands Attached Cryogel Disks

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