Hydrolysis of phosphate esters in aqueous solution promoted by cobalt(III)-tetraamine metallohydrolases: Chromatographic and spectroscopic studies

Fabrication of highly active phosphatase-like fluorescent cerium-doped carbon dots for in situ monitoring the hydrolysis of phosphate diesters

“…We used hydrolysis of phosphate diester as a model reaction for the cleavage of phosphorus bond of DNA and RNA. 40 Here, BNPP was chosen as a DNA model substrate, and phosphatase-like activity of the obtained CeCDs was explored based on the inner lter effect (IFE), i.e., the maximum absorption range of BNPP hydrolysis products (p-nitrophenol) partly overlaps with emission spectrum of the as-prepared CeCDs, resulting in an efficient uorescence quenching of the CeCDs. The rate of BNPP hydrolysis was calculated and the possible catalytic mechanism was proposed.…”