Hydrolysis of Peripheral Leucine Enkephalin in Allergic Asthma

Fuso, Lionello; Marini, Mario; Marzano, Marina; Nardecchia, Bernardino; Roda, L.Giorgio; Rossi, Paolo; Urbani, Alessandra

doi:10.1006/clim.1998.4667

Cited by 6 publications

(1 citation statement)

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“…Peptide metabolism, then, can be used as an indirect measure of endogenous enzyme activity and may indicate immune system deficiencies [2]. Leu-enkephalin has been widely utilized as a model compound to study peptidase activity, yet even this small peptide has three possible degradation pathways and several possible metabolites [1±4, 6,8]. The variety of metabolic pathways for Leu-enkephalin and other neuropeptides requires a general yet selective method of analysis if we are to understand the role these compounds play in biological signaling and physiological processes.…”

Section: Introductionmentioning

confidence: 99%

Detection of neuropeptides using on-capillary copper complexation and capillary electrophoresis with electrochemical detection

Gawron

Lunte

2000

Electrophoresis

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Capillary electrophoresis with electrochemical detection using a carbon fiber electrode in conjunction with on-capillary copper complexation was evaluated for the determination of peptides in standard and biological matrices. Peptides composed of 2-10 amino acids were investigated. A comparison was made between the responses obtained for peptides containing the oxidizable residue tyrosine and those obtained for their respective copper complexes. Electrochemical detection of non-tyrosine-containing peptides and a cyclic peptide was also demonstrated. A separation of leucine (Leu)-enkephalin and five metabolites was developed and then used for the investigation of Leu-enkephalin metabolism in plasma. The appearance of the des-tyrosine (des-Tyr) Leu-enkephalin, which cannot be detected directly at a carbon electrode, was monitored using the on-capillary complexation technique. Direct injection of the plasma sample was possible using this methodology.

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Section: Introductionmentioning

confidence: 99%