Hydrolysis of Magnetic Amylase-Imprinted Poly(ethylene-<i>co</i>-vinyl alcohol) Composite Nanoparticles

Lee, Mei-Hwa; Thomas, James L.; Chen, Yunchao; Wang, Hsuan-Yun; Lin, Hung-Yin

doi:10.1021/am201576y

Cited by 37 publications

(18 citation statements)

References 28 publications

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“…Lee et al included a-amylase in poly(ethyleneco-vinyl alcohol) droplets containing magnetic nanoparticles. 264 The solvent was removed and the enzyme washed out yielding a MIP containing a cavity that specifically recognises a-amylase. Newly bound amylase had 94% of the activity of free enzyme and the thus bound enzyme could be reused 29 times with 86% remaining activity.…”

Section: Starch Hydrolysismentioning

confidence: 99%

Immobilised enzymes in biorenewables production

et al. 2013

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Oils, fats, carbohydrates, lignin, and amino acids are all important raw materials for the production of biorenewables. These compounds already play an important role in everyday life in the form of wood, fabrics, starch, paper and rubber. Enzymatic reactions do, in principle, allow the transformation of these raw materials into biorenewables under mild and sustainable conditions. There are a few examples of processes using immobilised enzymes that are already applied on an industrial scale, such as the production of High-Fructose Corn Syrup, but these are still rather rare. Fortunately, there is a rapid expansion in the research efforts that try to improve this, driven by a combination of economic and ecological reasons. This review focusses on those efforts, by looking at attempts to use fatty acids, carbohydrates, proteins and lignin (and their building blocks), as substrates in the synthesis of biorenewables using immobilised enzymes. Therefore, many examples (390 references) from the recent literature are discussed, in which we look both at the specific reactions as well as to the methods of immobilisation of the enzymes, as the latter are shown to be a crucial factor with respect to stability and reuse. The applications of the renewables produced in this way range from building blocks for the pharmaceutical and polymer industry, transport fuels, to additives for the food industry. A critical evaluation of the relevant factors that need to be improved for large-scale use of these examples is presented in the outlook of this review.

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Section: Starch Hydrolysismentioning

confidence: 99%

Immobilised enzymes in biorenewables production

et al. 2013

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“…The performance of an immobilized enzyme depends greatly on the character and structure of the carrier materials . Numerous approaches have been developed for the immobilization of amylase using different support materials like poly (ethylene‐co‐vinyl alcohol) magnetic composite , mesoporous silica thin films , carboxymethyl cellulose‐silver nanoparticle , alumina , and polyaniline‐polyvinyl alcohol film .…”

Section: Introductionmentioning

confidence: 99%

Immobilization of α‐amylase on reactive modified fiber and its application for continuous starch hydrolysis in a packed bed bioreactor

Temoçin

2013

Starch Stärke

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In this study, the enzyme α‐amylase was immobilized on reactive modified poly (ethylene terephthalate) fiber. The activities of free α‐amylase and immobilized α‐amylase were compared in a batch starch hydrolysis system. Optimum pH and Michaelis–Menten constants were determined for both free α‐amylase and immobilized α‐amylase. The immobilization shifted the optimum pH to a higher level. The Michaelis–Menten constant values (Km) of the immobilized α‐amylase and free α‐amylase were obtained as 11.94 and 6.61 mg/mL, respectively. The experiments indicated that the immobilization increased the thermal stability of the α‐amylase. The immobilized α‐amylase, easily separated from the reaction media, could be used effectively for 15 cycles with 65% retention of the initial activity. Moreover, the immobilized α‐amylase was effectively used in packed bed bioreactor for continuous starch hydrolysis throughout 6.5 h without loss of activity. Different starches (e.g., potato and wheat) were hydrolyzed by the immobilized α‐amylase in continuous process. The results indicate a potential use of this immobilized enzyme system for the construction of packed bed bioreactors to be used in the hydrolysis of the starch.

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“…Lee et al [45] evaluated the immobilization of amylase on the magnetic molecularly imprinted polymers (MMIPs) composite NPs. The activities of both bound template and rebound enzyme were confirmed by measuring glucose production from starch hydrolysis, at different temperatures, for MMIPs with different compositions.…”

Section: α-Amylasementioning

confidence: 99%

Nanomaterials as novel supports for the immobilization of amylolytic enzymes and their applications: A review

Husain¹

2017

Biocatalysis

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Numerous types of nanoparticles and nanocomposites have successfully been employed for the immobilization and stabilization of amylolytic enzymes; α-amylases, β-amylases, glucoamylases and pullulanases. Nano-support immobilized amylolytic enzymes retained very high activity and yield of immobilization. The immobilization of these enzymes, particularly α-amylases and pullulanases, to the nanosupports is helpful in minimizing the problem of steric hindrances during binding of substrate to the active site of the enzyme. The majority of nano-support immobilized amylolytic enzymes exhibited very high resistance to inactivation induced by different kinds of physical and chemical denaturants and these immobilized enzyme preparations maintained very high activity on their repeated and continuous uses. Amylolytic enzymes immobilized on nano-supports have successfully been applied in food, fuel, textile, paper and pulp, detergent, environmental, medical, and analytical fields.

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Hydrolysis of Magnetic Amylase-Imprinted Poly(ethylene-co-vinyl alcohol) Composite Nanoparticles

Cited by 37 publications

References 28 publications

Immobilised enzymes in biorenewables production

Immobilised enzymes in biorenewables production

Immobilization of α‐amylase on reactive modified fiber and its application for continuous starch hydrolysis in a packed bed bioreactor

Nanomaterials as novel supports for the immobilization of amylolytic enzymes and their applications: A review

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