Hydrolysis of indoxyl acetate by Campylobacter species

Abstract

One hundred and twelve Campylobacter strains comprising 15 species and subspecies were examined for their ability to hydrolyze indoxyl acetate. All strains of C. coli, C. cryaerophila, C. fennelliae, and C. jejuni hydrolyzed the compound, whereas three strains of C. cinaedi were negative and a fourth was weakly positive. Representatives of all other species were negative. Organisms that hydrolyzed indoxyl acetate did so regardless of the medium used.

First finding of urease-positive thermophilic strains of Campylobacter in river water in the Far East, namely, in Japan and their phenotypic and genotypic characterization

“…Thus, these characteristics may possibly be common to all UPTC. Furthermore, we examined arylsulphatase and pyrazinamidase activities (Burnens and Nicolet 1993), as well as indoxyl acetate hydrolysis (Mills and Gherna 1987). As shown in Table 1, these two strains were positive for arylsulphatase and pyrazinamidase and negative for indoxyl acetate hydrolysis.…”

Routine identification of Campylobacter jejuni and Campylobacter coli from human stool samples

“…Oxidase test (Organon Teknika, Germany) and hip-purate hydrolysis test (Rosco, Denmark) were performed according to the manufacturers' instructions. Indoxyl acetate hydrolysis was tested according to Mills and Gherna [10]. Antimicrobial susceptibility testing was performed using a disk di¡usion test for nalidixic acid and cephalotin.…”

Occurrence of campylobacters in small domestic and laboratory animals

“…tested with Microscreen@ Campylobacter latex test (Mercia Diagnostics, Guildford) according to the manufacturer's instructions. A single, representative, presumptive campylobacter-positive colony from each sample, was subcultured from the filtration plate and further characterized by the following biochemical tests : growth in air, oxidase, catalase (Skirrow and Benjamin 1980), nitrate reduction (Cook 1950), H,S production (Roop et al 1984a), susceptibility to nalidixic acid and cephalothin (Skirrow and Benjamin 1980), hydrolysis of hippurate (Harvey 1980) and indoxyl acetate (Mills and Gherna 1987), growth at various temperatures and in the presence of 1% glycine (Lander and Gill 1985), and finally by DNA-DNA hybridization.…”