Hydrolysis of freshly prepared wheat starch fractions and commercial wheat starch using α-amylase

Sakintuna, Billur; Budak, Okan; Dik, T. A.; Yöndem‐Makascıoǧlu, Füsun; Kıncal, N. Suzan

doi:10.1080/00986440302118

Cited by 7 publications

(2 citation statements)

References 18 publications

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“…However, K. oxytoca KMS006 does not efficiently ferment cassava starch owing to the low expression of amylolytic enzymes. During starch hydrolysis, α‐amylase hydrolyzes the starch to maltose, which can be further hydrolyzed into glucose subunits by glucoamylase activity 21,22 . A high addition of enzymes for starch hydrolysis may increase the production costs associated with the medium for 2,3‐BD fermentation.…”

Section: Introductionmentioning

confidence: 99%

Sequential coupling of enzymatic hydrolysis and fermentation platform for high yield and economical production of 2,3‐butanediol from cassava by metabolically engineered Klebsiella oxytoca

Khunnonkwao

Jantama

et al. 2020

J of Chemical Tech & Biotech

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BACKGROUND 2,3‐butanediol (2,3‐BD) is one of the important platform chemicals used for biofuels and biochemicals. To make an economically feasible production of 2,3‐BD, an optimization process with a cheap substrate is required. The best conditions for enzymatic hydrolysis and fermentation were investigated to achieve high concentration and yield of 2,3‐BD from cassava starch while reducing the production cost regarding enzyme usage, energy input and medium preparation. RESULTS A 2.5 L bioreactor containing 1 L working volume of sterilized and gelatinized starch (130 g L−1) was placed at room temperature (25 °C). When the temperature of the starch solution reached 85 °C, α‐amylase (200 U g−1 starch) was added to the solution. The enzymatic reaction was allowed to proceed while the temperature of the solution was simultaneously dampened to 45 °C within 1 h. The reaction was further maintained at 45 °C for another 2 h. After hydrolysis, 64.8 g L−1 2,3‐BD was produced with a yield of 0.46 g g−1, productivity of 1.25 g L−1 h−1 and few by‐products by Klebsiella oxytoca KMS006. Further improvements of 2,3‐BD (92.5 g L−1) and yield (0.49 g g−1) were observed in fed‐batch mode under a constant feeding rate of 3.5 g starch h−1. CONCLUSION With our developed strategy, costs related to energy consumption and medium preparation were reduced by using heat generated by autoclave as well as by neglecting the use of glucoamylase for hydrolysis of cassava starch. The highest yield of 2,3‐BD (0.49 g g−1) was achieved, which is 98% of theoretical maximum. The process may be applied to the economical production of 2,3‐BD. © 2020 Society of Chemical Industry

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Section: Introductionmentioning

confidence: 99%

Sequential coupling of enzymatic hydrolysis and fermentation platform for high yield and economical production of 2,3‐butanediol from cassava by metabolically engineered Klebsiella oxytoca

Khunnonkwao

Jantama

et al. 2020

J of Chemical Tech & Biotech

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“…Enzymatic hydrolysis is preferred over acid hydrolysis because of its advantages, such as no formation of 5‐(hydroxymethyl)‐2‐furaldehyde, which is formed from the dehydration of d ‐glucose, inhibiting yeast growth during fermentation , and lower energy consumption by milder operating conditions . In this process, α‐amylase hydrolyzes the starch chain producing dextrins, which can be hydrolyzed in maltoses by β‐amylase or in glucose by the glucoamylases .…”

Section: Introductionmentioning

confidence: 99%

Conversion of cassava starch to ethanol and a byproduct under different hydrolysis conditions

et al. 2015

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Cassava starch can be converted into sugars by enzymatic hydrolysis to be used for ethanol production. In this work, the studied variables were starch and enzymes concentration seeking a higher conversion of the reducing sugars routine. Two series of experiments were conducted following the steps of gelatinization, liquefaction, and saccharification, ending with the fermentation step. It was found that a 100–250 times increase in α‐amylase concentration results in a 23% increase in the starch yield. Therefore, an optimal enzyme concentration could be found. The glucoamylase enzyme has proved to be more effective, evaluated by the rate of starch conversion (k), at a lower concentration of starch (10%), and enzyme (10 U/g). In simultaneous saccharification and fermentation (SSF), the hydrolysis yield was 33% higher than in a conventional process, achieving efficiencies higher than 70%. The solid residue separated after the SSF process proved to be a high‐quality animal feed additive.

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Binding interactions of α-amylase with starch granules: The influence of supramolecular structure and surface area

Warren

Royall

Gaisford

et al. 2011

Carbohydrate Polymers

124

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Hydrolysis of freshly prepared wheat starch fractions and commercial wheat starch using α-amylase

Cited by 7 publications

References 18 publications

Sequential coupling of enzymatic hydrolysis and fermentation platform for high yield and economical production of 2,3‐butanediol from cassava by metabolically engineered Klebsiella oxytoca

Sequential coupling of enzymatic hydrolysis and fermentation platform for high yield and economical production of 2,3‐butanediol from cassava by metabolically engineered Klebsiella oxytoca

Conversion of cassava starch to ethanol and a byproduct under different hydrolysis conditions

Binding interactions of α-amylase with starch granules: The influence of supramolecular structure and surface area

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