Hydrolysis and transgalactosylation catalysed by β-galactosidase from brush border membrane vesicles isolated from pig small intestine: A study using lactulose and its mixtures with lactose or galactose as substrates

Julio-González, Lesbia Cristina; Hernández‐Hernández, Oswaldo; Moreno, F. Javier; Jimeno, María Luisa; Doyagüez, Elisa G.; Olano, Agustı́n; Corzo, Nieves

doi:10.1016/j.foodres.2019.108811

Cited by 9 publications

(2 citation statements)

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“…Isolation by Semi-Preparative Chromatography. Trehalose-derived oligosaccharides were isolated by hydrophilic interaction liquid chromatography equipped with a refractive index detector (HILIC-RID), following the method of Julio-Gonzaĺez et al 32 Sample separation was carried out by a semi-preparative ZORBAX NH2 column (PrepHT cartridge 250 × 21.2 mm, 7 μm particle size) (Agilent), using acetonitrile/water (70:30, v/v) as a mobile phase, at a flow rate of 21 mL min −1 for 45 min at 25 °C. Two milliliters was repeatedly injected.…”

Section: Methodsmentioning

confidence: 99%

Enzymatic Synthesis and Structural Characterization of Novel Trehalose-Based Oligosaccharides

Gallego-Lobillo

Doyagüez

Jimeno

et al. 2021

J. Agric. Food Chem.

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Trehalose, α- d -glucopyranosyl-(1↔1)-α- d -glucopyranoside, is a disaccharide with multiple effects on the human body. Synthesis of new trehalose derivatives was investigated through transgalactosylation reactions using β-galactosidase from four different species. β-galactosidases from Bacillus circulans ( B. circulans ) and Aspergillus oryzae ( A. oryzae ) were observed to be the best biocatalysts, using lactose as the donor and trehalose as the acceptor. Galactosyl derivatives of trehalose were characterized using nuclear magnetic resonance spectroscopy. Trisaccharides were the most abundant oligosaccharides obtained followed by the tetrasaccharide fraction (19.5% vs 8.2% carbohydrates). Interestingly, the pentasaccharide [β-Gal p -(1→4)] 3 -trehalose was characterized for the first time. Greater oligosaccharide production was observed using β-galactosidase from B. circulans than that obtained from A. oryzae , where the main structures were based on galactose monomers linked by β-(1→6) and β-(1→4) bonds with trehalose in the ending. These results indicate the feasibility of commercially available β-galactosidases for the synthesis of trehalose-derived oligosaccharides, which might have functional properties, excluding the adverse effects of the single trehalose.

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Section: Methodsmentioning

confidence: 99%

Enzymatic Synthesis and Structural Characterization of Novel Trehalose-Based Oligosaccharides

Gallego-Lobillo

Doyagüez

Jimeno

et al. 2021

J. Agric. Food Chem.

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“…b-Galactosidase (EC 3.2.1.23) hydrolyzes the glycosidic bond in b-galactosides and finds applications in the food industry [1,2]. The trans-glycosylation property of bgalactosidase (b-gal) is widely used to produce various galactosylated products and prebiotics such as GOS and lactulose [3][4][5][6][7]. The b-gal enzyme is produced intracellularly by many bacteria and yeast, a major constraint for industrial production [1,8].…”

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confidence: 99%

A New Extracellular β-Galactosidase Producing Kluyveromyces sp. PCH397 from Yak Milk and Its Applications for Lactose Hydrolysis and Prebiotics Synthesis

Nag

Kumar

et al. 2021

Indian J Microbiol

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b-Galactosidase is a crucial glycoside hydrolase enzyme with potential applications in the dairy, food, and pharmaceutical industries. The enzyme is produced in the intracellular environment by bacteria and yeast. The present study reports yeast Kluyveromyces sp. PCH397 isolated from yak milk, which has displayed extracellular bgalactosidase activity in cell-free supernatant through the growth phase. To investigate further, cell counting and methylene blue staining of culture collected at different growth stages were performed and suggested for possible autolysis or cell lysis, thereby releasing enzymes into the extracellular medium. The maximum enzyme production (9.94 ± 2.53U/ml) was achieved at 37 °C in a modified deMan, Rogosa, and Sharpe (MRS) medium supplemented with lactose (1.5%) as a carbon source. The enzyme showed activity at a wide temperature range (4-50 °C), maximum at 50 °C in neutral pH (7.0). In addition to the hydrolysis of lactose (5.0%), crude b-galactosidase also synthesized vital prebiotics (i.e., lactulose and galactooligosaccharides (GOS)). Additionally, b-fructofuranosidase (FFase) activity in the culture supernatant ensued the synthesis of a significant prebiotic, fructo-oligosaccharides (FOS). Hence, the unique features such as extracellular enzymes production, efficient lactose hydrolysis, and broad temperature functionality by yeast isolate PCH397 are of industrial relevance. In conclusion, the present study unrevealed for the first time, extracellular production of bgalactosidase from a new yeast source and its applications in milk lactose hydrolysis and synthesis of valuable prebiotics of industrial importance.

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In vitro and in vivo digestibility of prebiotic galactooligosacharides synthesized by β‐galactosidase from Lactobacillus delbruecki subsp. bulgaricusCRL450

Fara,

Hernández Hernández,

Palacios

et al. 2024

J Sci Food Agric

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BackgroundTo date, the general belief that prebiotics reach the colon without any alterations is challenged. Some in vitro and in vivo studies have demonstrated that ‘non‐digestible’ oligosaccharides are digested to different degrees depending on their structural composition. In this work, we compared different methods to assess the digestibility of oligosaccharides synthesized by β‐galactosidase (β‐gal) of Lactobacillus delbruecki subsp. bulgaricus CRL450 (CRL450‐β‐gal) from lactose, lactulose and lactitol.ResultsIn simulated gastrointestinal fluid (SGIF) method no changes were observed. However, the oligosaccharides synthesized by CRL450‐β‐gal were partially hydrolyzed in vitro, depending on their structure and composition, with rat small intestinal extract (RSIE) and small intestinal brush‐border membrane vesicles (BBMV) from pig. Digestion of some oligosaccharides increased when mixtures were fed to C57BL/6 mice used as in vivo model, however, lactulose‐oligosaccharides were the most resistant to the physiological conditions of mice. In general β(1 → 6) linked products showed higher resistance compared to β(1 → 3) oligosaccharides.ConclusionIn vitro digestion methods, without disaccharidases, may underestimate the importance of carbohydrates hydrolysis in the small intestine. Although BVMM and RSIE digestion assays are appropriate in vitro methods for these studies, in vivo studies remain the most reliable for understanding what actually happens in the digestion of oligosaccharides.This article is protected by copyright. All rights reserved.

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Hydrolysis and transgalactosylation catalysed by β-galactosidase from brush border membrane vesicles isolated from pig small intestine: A study using lactulose and its mixtures with lactose or galactose as substrates

Cited by 9 publications

References 31 publications

Enzymatic Synthesis and Structural Characterization of Novel Trehalose-Based Oligosaccharides

Enzymatic Synthesis and Structural Characterization of Novel Trehalose-Based Oligosaccharides

A New Extracellular β-Galactosidase Producing Kluyveromyces sp. PCH397 from Yak Milk and Its Applications for Lactose Hydrolysis and Prebiotics Synthesis

In vitro and in vivo digestibility of prebiotic galactooligosacharides synthesized by β‐galactosidase from Lactobacillus delbruecki subsp. bulgaricusCRL450

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