Hydrological regulation of Vibrio dynamics in a tropical monsoonal estuary: a classification and regression tree approach

Krishna, K. S.; Veettil, Vipindas Puthiya; Anas, Abdulaziz; Nair, Shanta

doi:10.1007/s11356-020-10486-9

Cited by 11 publications

(9 citation statements)

References 55 publications

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“…Compared to the environmental temperature, salinity is a more compelling critical factor (Takemura et al, 2014). Most experiments showed a significant positive correlation between Vibrio and salinity (Perkins et al, 2014;Krishna et al, 2021), consistent with our results. According to a study, the potential opportunistic pathogenic V. alginolyticus and V. parahaemolyticus are halotolerant (Mookerjee et al, 2015), and thus the 16S NGS results of this experiment showed that their distribution patterns showed dominance at high salinity.…”

Section: Influencing Factors On the Distribution Of Potential Pathoge...supporting

confidence: 91%

16S Next-generation sequencing and quantitative PCR reveal the distribution of potential pathogens in the Liaohe Estuary

et al. 2022

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The existence of potentially pathogenic bacteria seriously threatens aquatic animals and human health. Estuaries are closely related to human activities, and the detection of pathogens is important for aquaculture and public health. However, monitoring only indicator microorganisms and pathogens is not enough to accurately and comprehensively estimate water pollution. Here, the diversity of potentially pathogenic bacteria in water samples from the Liaohe estuary was profiled using 16S next-generation sequencing (16S NGS) and quantitative polymerase chain reaction (qPCR) analysis. The results showed that the dominant genera of environmental pathogens were Pseudomonas, Vibrio, Mycobacterium, Acinetobacter, Exiguobacterium, Sphingomonas, and Legionella, and the abundance of enteric pathogens was significantly less than the environmental pathogens, mainly, Citrobacter, Enterococcus, Escherichia-Shigella, Enterobacter, Bacteroides. The qPCR results showed that the 16S rRNA genes of Vibrio were the most abundant, with concentrations between 7.06 and 9.48 lg copies/L, followed by oaa gene, fliC gene, trh gene, and uidA gene, and the temperature and salinity were the main factors affecting its abundance. Variance partitioning analysis (VPA) analysis of spatial factors on the potential pathogen’s distribution (19.6% vs 5.3%) was greater than environmental factors. In addition, the co-occurrence analysis of potential pathogens in the estuary revealed significant co-occurrence among the opportunistic pathogens Testosteronemonas, Brevimonas vesicularis, and Pseudomonas putida. Our findings provide an essential reference for monitoring and occurrence of potentially pathogenic bacteria in estuaries.

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Section: Influencing Factors On the Distribution Of Potential Pathoge...supporting

confidence: 91%

16S Next-generation sequencing and quantitative PCR reveal the distribution of potential pathogens in the Liaohe Estuary

et al. 2022

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“…The high population density (860 persons/km² according to census 2011 data), less availability of safe drinking water (Figure 2), prolonged monsoon and prevalence of Vibrio cholerae in the surface water makes the region endemic to cholera. The environmental monitoring studies conducted in this area also confirmed the prevalence of Vibrio cholerae in the aquatic environments thorough out the year [16]. Water associated diseases are considered one of the major reasons for reduced man working days in the region [17].…”

Section: Resultsmentioning

confidence: 68%

Multiple Drug-Resistant Vibrio Cholerae Responsible for Cholera Outbreak among Migrant Domestic Workers in Kerala, South India

Krishna

Anas

Kuttan

et al. 2021

The 1st International Electronic Conference on Microbiology

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Cholera outbreaks are prevalent in countries with a low Human Development Index (HDI) where people have limited access to safe drinking water, sanitation and hygiene (WASH). Intriguingly, the state of Kerala which records the highest HDI in India is endemic to cholera. We discuss the epidemiology of a cholera outbreak reported among migrant workers of Kerala in 2017. Virulence genes of Vibrio cholerae, toxR and ctxA, were detected in the river and ground water samples collected from the outbreaks sites which indicates need of enhanced awareness on WASH practices among migrant workers. The pathogenic Vibrio cholerae isolated from four patients in two districts had a similar DNA band pattern when analysed using repetitive extragenic palindromic-PCR (BOX), which indicates their single clonal origin. The four isolates were serotyped as O1 Ogawa. These isolates were resistant to multiple antibiotics including the carbapenem like imipenem. Nevertheless, isolates of these pathogenic bacteria were susceptible to tetracycline, identical to clinical isolates of Vibrio cholera reported earlier from this region. The current study highlights the importance of generating awareness on WASH protocols among migrant workers to prevent the outbreaks and considers community-based data as socioeconomic variable to predict the incidence of cholera.

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“…2), prolonged monsoon and prevalence of V. cholerae in the surface water makes the region endemic to cholera. The environmental monitoring studies conducted in this area also confirmed the prevalence of V. cholerae in the aquatic environments thorough out the year [16]. Water associated diseases are considered as one of the major reasons for reduced man working days in the region [17].…”

Section: Resultsmentioning

confidence: 66%

Multiple drug-resistant <em>Vibrio cholerae </em>responsible for cholera outbreak among migrant domestic workers in Kerala, South India

Anas

Krishna

et al. 2020

Proceedings of 1st International Electronic Conference on Microbiology

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Cholera outbreaks are prevalent in countries with low Human Development Index (HDI) where people have limited access to safe drinking water, sanitation and hygiene (WASH). Intriguingly, state of Kerala which records the highest HDI in India is endemic to cholera. We discuss the epidemiology of a cholera outbreak reported among migrant workers of Kerala in 2017. Virulence genes of Vibrio cholerae, tox R and ctx A, were detected in the river and ground water samples collected from the outbreaks sites which indicates need of enhanced awareness on WASH practices among migrant workers. The pathogenic Vibrio cholerae isolated from four patients in two districts had a similar DNA band pattern when analysed using repetitive extragenic palindromic-PCR (BOX), which indicates their single clonal origin. The four isolates were serotyped as O1 Ogawa. These isolates were resistant to multiple antibiotics including the carbapenem like imipenem. Nevertheless, isolates of these pathogenic bacteria were susceptible to tetracycline identical to clinical isolates of Vibrio cholera reported earlier from this region. Current study highlights the importance of generating awareness on WASH protocols among migrant workers to prevent the outbreaks and considers community-based data as socioeconomic variable to predict the incidence of cholera.

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Hydrological regulation of Vibrio dynamics in a tropical monsoonal estuary: a classification and regression tree approach

Cited by 11 publications

References 55 publications

16S Next-generation sequencing and quantitative PCR reveal the distribution of potential pathogens in the Liaohe Estuary

16S Next-generation sequencing and quantitative PCR reveal the distribution of potential pathogens in the Liaohe Estuary

Multiple Drug-Resistant Vibrio Cholerae Responsible for Cholera Outbreak among Migrant Domestic Workers in Kerala, South India

Multiple drug-resistant <em>Vibrio cholerae </em>responsible for cholera outbreak among migrant domestic workers in Kerala, South India

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Hydrological regulation of Vibrio dynamics in a tropical monsoonal estuary: a classification and regression tree approach

Cited by 11 publications

References 55 publications

16S Next-generation sequencing and quantitative PCR reveal the distribution of potential pathogens in the Liaohe Estuary

16S Next-generation sequencing and quantitative PCR reveal the distribution of potential pathogens in the Liaohe Estuary

Multiple Drug-Resistant Vibrio Cholerae Responsible for Cholera Outbreak among Migrant Domestic Workers in Kerala, South India

Multiple drug-resistant &lt;em&gt;Vibrio cholerae &lt;/em&gt;responsible for cholera outbreak among migrant domestic workers in Kerala, South India

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Multiple drug-resistant <em>Vibrio cholerae </em>responsible for cholera outbreak among migrant domestic workers in Kerala, South India