Objective: To monitor and provide the first-hand information on the groundwater quality by assessing spatio-temporal variations of physicochemical characteristics and heavy metals in Aizawl, Mizoram, an Indo Burma biodiversity hotspot region. Methods: Physico-chemical characteristics of groundwater were analyzed through standard methodology following American Public Health Association (APHA) while heavy metals were analyzed using Atomic Absorption Spectrophotometer (AAS). Findings: Results revealed that several physico-chemical characteristics were observed within the range of regulatory or prescribed limits, except PO 4 3and N-NH 3 . To this end, the ranges of physico-chemical characteristics such as Total Alkalinity ((TA) 9.25 -250.9 mg/L), Total Hardness ((TH)11-242.5 mg/L), Calcium (2.2-71 mg/L), Phosphate ((PO 4 3-) 0-0.63 mg/L), Ammonia ((N-NH 3 )0.056-1.816 mg/L), Nitrate ((N-NO 2 ) 0.003-0.88 mg/L), and heavy metals, specifically Zinc ((Zn)0.2.196 mg/L) and Iron ((Fe)0-1.162 mg/L) were recorded. The present study provides first-hand information on physico-chemical characteristics, especially heavy metals in groundwater sample of Aizawl which is an integral landscape of Indo Burma biodiversity hotspot. Novelty: Groundwater pollution can be maintained in Aizawl through regular monitoring and application of green chemicals as well as phytoremediation technology to sustain the potable water quality for human well-being/-health.