Hydrogenophaga defluvii sp. nov. and Hydrogenophaga atypica sp. nov., isolated from activated sludge

Kämpfer, Peter; Schulze, Renate; Jäckel, Udo; Malik, K. A.; Amann, Rudolf; Spring, Stefan

doi:10.1099/ijs.0.03041-0

Cited by 94 publications

(52 citation statements)

References 19 publications

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“…are facultative heterotrophs frequently associated with wastewater treatment (5,20,22). Certain Hydrogenophaga species are capable of thiosulfate oxidation (18); thus, the Hydrogenophaga-related phylotype detected in this study and a previous study (12) may participate in sulfide oxidation in the FBR.…”

mentioning

confidence: 80%

Identification of Bacteria Potentially Responsible for Oxic and Anoxic Sulfide Oxidation in Biofilters of a Recirculating Mariculture System

Cytryn

Rijn

Schramm

et al. 2005

Appl Environ Microbiol

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Bacteria presumably involved in oxygen-or nitrate-dependent sulfide oxidation in the biofilters of a recirculating marine aquaculture system were identified using a new application of reverse transcription-PCR denaturing gradient gel electrophoresis (DGGE) analysis termed differential-transcription (DT)-DGGE. Biofilter samples were incubated in various concentrations of sulfide or thiosulfate (0 to 5 mM) with either oxygen or nitrate as the sole electron acceptor. Before and after short-term incubations (10 to 20 h), total DNA and RNA were extracted, and a 550-bp fragment of the 16S rRNA genes was PCR amplified either directly or after reverse transcription. DGGE analysis of DNA showed no significant change of the original microbial consortia upon incubation. In contrast, DGGE of cDNA revealed several phylotypes whose relative band intensities markedly increased or decreased in response to certain incubation conditions, indicating enhanced or suppressed rRNA transcription and thus implying metabolic activity under these conditions. Specifically, species of the gammaproteobacterial genus Thiomicrospira and phylotypes related to symbiotic sulfide oxidizers could be linked to oxygen-dependent sulfide oxidation, while members of the Rhodobacteraceae (genera Roseobacter, Rhodobacter, and Rhodobium) were putatively active in anoxic, nitrate-dependent sulfide oxidation. For all these organisms, the physiology of their closest cultured relatives matches their DT-DGGE-inferred function. In addition, higher band intensities following exposure to 5 mM sulfide and nitrate were observed for Thauera-, Hydrogenophaga-, and Dethiosulfovibrio-like phylotypes. For these genera, nitrate-dependent sulfide oxidation has not been documented previously and therefore DT-DGGE might indicate a higher relative tolerance to high sulfide concentrations than that of other community members. We anticipate that DT-DGGE will be of general use in tracing functionally equivalent yet phylogenetically diverse microbial populations in nature.Zero-discharge marine aquaculture (mariculture) systems provide a solution for the environmentally detrimental effects of conventional mariculture (16). These closed systems utilize biofilters to convert the main contaminants from the process water to harmless gases. Treatment encompasses oxidation of ammonia to nitrate in an aerobic trickling filter and anaerobic removal of organic matter and nitrate by denitrification in a parallel purification loop comprising a digestion/sedimentation basin (DB) and a fluidized-bed reactor (FBR) (16).The high organic load entering the DB creates sharp redox potential gradients with extensive sulfate reduction within settled sludge. In these nitrate-depleted zones, sulfide concentrations can reach values exceeding 5,000 M (11). Due to extensive sulfide oxidation, sulfide concentrations in the overlying water of the basin are generally more than 1 order of magnitude below these values (11). Remaining sulfide from the DB effluent is oxidized in the organic carbon-limited FBR (12...

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mentioning

confidence: 80%

Identification of Bacteria Potentially Responsible for Oxic and Anoxic Sulfide Oxidation in Biofilters of a Recirculating Mariculture System

Cytryn

Rijn

Schramm

et al. 2005

Appl Environ Microbiol

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“…in MFCs fed with short-chain fatty acids is sometimes reported (Teng et al, 2010;Xing et al, 2010), their ecophysiological roles in the MFCs are largely unknown. In general, members of the genus Hydrogenophaga are known to be aerobic bacteria that can oxidize hydrogen but not acetate (Kämpfer et al, 2005). For archaeal community, only two same bands (A-1 and A-2) were detected from all samples of anodic biofilms and culture fluid, both of which are closely related to Methanosarcina sp.…”

Section: Syntrophic Acetate-oxidizing Microbes In Mfcsmentioning

confidence: 99%

Acetate oxidation by syntrophic association between Geobacter sulfurreducens and a hydrogen-utilizing exoelectrogen

Kimura

Okabe

2013

The ISME Journal

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Anodic microbial communities in acetate-fed microbial fuel cells (MFCs) were analyzed using stableisotope probing of 16S rRNA genes followed by denaturing gradient gel electrophoresis. The results revealed that Geobacter sulfurreducens and Hydrogenophaga sp. predominated in the anodic biofilm. Although the predominance of Geobacter sp. as acetoclastic exoelectrogens in acetate-fed MFC systems has been often reported, the ecophysiological role of Hydrogenophaga sp. is unknown. Therefore, we isolated and characterized a bacterium closely related to Hydrogenophaga sp. (designated strain AR20). The newly isolated strain AR20 could use molecular hydrogen (H 2 ), but not acetate, with carbon electrode as the electron acceptor, indicating that the strain AR20 was a hydrogenotrophic exoelectrogen. This evidence raises a hypothesis that acetate was oxidized by G. sulfurreducens in syntrophic cooperation with the strain AR20 as a hydrogen-consuming partner in the acetate-fed MFC. To prove this hypothesis, G. sulfurreducens strain PCA was cocultivated with the strain AR20 in the acetate-fed MFC without any dissolved electron acceptors. In the coculture MFC of G. sulfurreducens and strain AR20, current generation and acetate degradation were the highest, and the growth of strain AR20 was observed. No current generation, acetate degradation and cell growth occurred in the strain AR20 pure culture MFC. These results show for the first time that G. sulfurreducens can oxidize acetate in syntrophic cooperation with the isolated Hydrogenophaga sp. strain AR20, with electrode as the electron acceptor.

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“…Three prominent DGGE bands present over the entire experiment represented Hydrogenophaga and Herbaspirillum species. These genera are known for their diverse metabolic phenotypes and ability to use a wide range of low-molecular-weight organic matter (21,36). On the other hand, OTUs prominent during the stable growth phase were affiliated with bacterial groups that may perform rather specialized functions in a microbial community.…”

Section: Microbial Community Successionmentioning

confidence: 99%

Biophysical Controls on Community Succession in Stream Biofilms

Besemer

Singer

Limberger

et al. 2007

Appl Environ Microbiol

211

180

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Biofilm formation is controlled by an array of coupled physical, chemical, and biotic processes. Despite the ecological relevance of microbial biofilms, their community formation and succession remain poorly understood. We investigated the effect of flow velocity, as the major physical force in stream ecosystems, on biofilm community succession (as continuous shifts in community composition) in microcosms under laminar, intermediate, and turbulent flow. Flow clearly shaped the development of biofilm architecture and community composition, as revealed by microscopic investigation, denaturing gradient gel electrophoresis (DGGE) analysis, and sequencing. While biofilm growth patterns were undirected under laminar flow, they were clearly directed into ridges and conspicuous streamers under turbulent flow. A total of 51 biofilm DGGE bands were detected; the average number ranged from 13 to 16. Successional trajectories diverged from an initial community that was common in all flow treatments and increasingly converged as biofilms matured. We suggest that this developmental pattern was primarily driven by algae, which, as "ecosystem engineers," modulate their microenvironment to create similar architectures and flow conditions in all treatments and thereby reduce the physical effect of flow on biofilms. Our results thus suggest a shift from a predominantly physical control to coupled biophysical controls on bacterial community succession in stream biofilms.

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Hydrogenophaga defluvii sp. nov. and Hydrogenophaga atypica sp. nov., isolated from activated sludge

Cited by 94 publications

References 19 publications

Identification of Bacteria Potentially Responsible for Oxic and Anoxic Sulfide Oxidation in Biofilters of a Recirculating Mariculture System

Identification of Bacteria Potentially Responsible for Oxic and Anoxic Sulfide Oxidation in Biofilters of a Recirculating Mariculture System

Acetate oxidation by syntrophic association between Geobacter sulfurreducens and a hydrogen-utilizing exoelectrogen

Biophysical Controls on Community Succession in Stream Biofilms

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