Hydrogen uptake in Nostoc sp. strain PCC 73102. Cloning and characterization of a hupSL homologue

Oxelfelt, Fredrik; Tamagnini, Paula; Lindblad, Peter

doi:10.1007/s002030050571

Cited by 51 publications

(58 citation statements)

References 29 publications

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“…While the uptake hydrogenase is present in all nitrogenfixing strains tested so far, the bidirectional enzyme is distributed among both nitrogen-fixing and non-nitrogen-fixing cyanobacteria (although it is not a universal cyanobacterial enzyme) (192). The molecular masses indicated for the uptake hydrogenase subunits are mean values calculated from the deduced amino acid sequences of Anabaena strain PCC 7120 (39), Nostoc strain PCC 73102 (150), and A. variabilis ATCC 29413 (79), while the values for the subunits of the bidirectional enzyme are based on data exclusively from A. variabilis ATCC 29413 (173).…”

Section: Nitrogenasesmentioning

confidence: 99%

Hydrogenases and Hydrogen Metabolism of Cyanobacteria

Tamagnini

Axelsson

Lindberg

et al. 2002

Microbiol Mol Biol Rev

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464

372

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Cyanobacteria may possess several enzymes that are directly involved in dihydrogen metabolism: nitrogenase(s) catalyzing the production of hydrogen concomitantly with the reduction of dinitrogen to ammonia, an uptake hydrogenase (encoded by hupSL) catalyzing the consumption of hydrogen produced by the nitrogenase, and a bidirectional hydrogenase (encoded by hoxFUYH) which has the capacity to both take up and produce hydrogen. This review summarizes our knowledge about cyanobacterial hydrogenases, focusing on recent progress since the first molecular information was published in 1995. It presents the molecular knowledge about cyanobacterial hupSL and hoxFUYH, their corresponding gene products, and their accessory genes before finishing with an applied aspect—the use of cyanobacteria in a biological, renewable production of the future energy carrier molecular hydrogen. In addition to scientific publications, information from three cyanobacterial genomes, the unicellular Synechocystis strain PCC 6803 and the filamentous heterocystous Anabaena strain PCC 7120 and Nostoc punctiforme (PCC 73102/ATCC 29133) is included

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Section: Nitrogenasesmentioning

confidence: 99%

Hydrogenases and Hydrogen Metabolism of Cyanobacteria

Tamagnini

Axelsson

Lindberg

et al. 2002

Microbiol Mol Biol Rev

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464

372

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“…Ligation into the vector pGEM 3Zf(+) (Promega), transformation and screening (using the probe GhupS; see also Fig. 1a) were performed as described previously (Oxelfelt et al, 1998). Positive clones were detected using a Typhoon 8600 Variable Mode imager (Amersham Biosciences).…”

Section: Primer Sequence 5 §R3 § Referencesmentioning

confidence: 99%

“…All cyanobacteria examined so far contain an uptake, a bi-directional or both the hydrogenases (Schmitz et al, 1995(Schmitz et al, , 2002Boison et al, 1996;Tamagnini et al, 2000Tamagnini et al, , 2002Sheremetieva et al, 2002; Schütz et al, 2004). Moreover, the uptake-type enzyme has been found in all nitrogen fixing cyanobacterial strains studied to date (Carrasco & Golden, 1995;Oxelfelt et al, 1998;Happe et al, 2000;Tamagnini et al, 2000Tamagnini et al, , 2002 Schütz et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Characterization and transcriptional analysis of hupSLW in Gloeothece sp. ATCC 27152: an uptake hydrogenase from a unicellular cyanobacterium

et al. 2004

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The structural genes (hupSL) encoding an uptake hydrogenase in the unicellular cyanobacterium Gloeothece sp. ATCC 27152, a strain capable of aerobic N 2 fixation, were identified and sequenced. 39-RACE experiments uncovered the presence of an additional ORF 184 bp downstream of hupL, showing a high degree of sequence identity with a gene encoding an uptake-hydrogenase-specific endopeptidase (hupW ) in other cyanobacteria. In addition, the transcription start point was identified 238 bp upstream of the hupS translational start. RT-PCR experiments revealed that hupW is co-transcribed with the uptake hydrogenase structural genes in Gloeothece sp. ATCC 27152. In addition, Northern hybridizations clearly showed that hupSLW are transcribed under nitrogen fixing conditions, but not in the presence of combined nitrogen. A putative NtcA binding site was identified in the promoter region upstream of hupS, centred at "41?5 bp with respect to the transcription start point. Electrophoretic retardation of a labelled DNA fragment (harbouring the putative NtcA-binding motif) was significantly affected by an Escherichia coli cell-free extract containing overexpressed NtcA, suggesting that NtcA is involved in the transcriptional regulation of hupSLW. INTRODUCTIONSeveral species of bacteria and cyanobacteria are capable of N 2 fixation. During the N 2 fixation process H 2 is formed as a by-product. This nitrogenase-dependent H 2 production is often compromised by the presence of an uptake hydrogenase (encoded by hupSL) that rapidly consumes the H 2 generated. In addition, a bi-directional enzyme (encoded by hoxEFUYH) may be present which, depending on the growth conditions, may display the capacity of both producing and consuming H 2 (Lambert & Smith, 1981;Houchins, 1984;Schmitz et al., 2002;Tamagnini et al., 2002). All cyanobacteria examined so far contain an uptake, a bi-directional or both the hydrogenases (Schmitz et al., 1995(Schmitz et al., , 2002Boison et al., 1996;Tamagnini et al., 2000Tamagnini et al., , 2002Sheremetieva et al., 2002; Schütz et al., 2004). Moreover, the uptake-type enzyme has been found in all nitrogen fixing cyanobacterial strains studied to date (Carrasco & Golden, 1995;Oxelfelt et al., 1998;Happe et al., 2000;Tamagnini et al., 2000Tamagnini et al., , 2002 Schütz et al., 2004).The first data on cyanobacterial hupSL transcription appeared in 1995 (Carrasco & Golden, 1995). RT-PCR experiments on Anabaena/Nostoc sp. strain PCC 7120 demonstrated that hupL transcription coincides with the formation of heterocysts. Subsequent studies, in other filamentous strains, have confirmed the induction of an hupL transcript under nitrogen-fixing conditions only (Axelsson et al., 1999;Happe et al., 2000; Hansel et al., 2001). One exception is Anabaena variabilis ATCC 29413, where a low level of hupL expression has been detected in vegetative cells grown with the addition of ammonia (Boison et al., 2000). In all the cyanobacterial strains 3Present address: Department of Physiological Botany, Evolutionary Biology Centre,...

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“…Inactivation of the xisF gene prevents N # fixation, but has no effect on heterocyst differentiation and pattern (Carrasco et al, 1994). Indeed, both the 11-kb (Oxelfelt et al, 1998 ;Bo$ hme, 1998) and the 55-kb (Haselkorn, 1992 ;Thiel et al, 1999) elements are absent from the genomes of a number of Anabaena and Nostoc strains.…”

Section: Heterocyst Developmentmentioning

confidence: 99%