Hydrogen Uptake (Hydrogenase) Activity of Rhizobium Japonicum Strains Forming Nodules in Soybean Production Areas of the U.S.A.

Lim, S. T.; Uratsu, Sandra L.; Weber, D. F.; Keyser, H. H.

doi:10.1007/978-1-4684-3953-3_15

Cited by 7 publications

(2 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(1,16,19,25). In 1979, a large survey of R. japonicum isolates from 28 states which make up the major U.S. soybean production area showed that a majority (>75%) were lacking the Hup system (18).…”

mentioning

confidence: 99%

Rhizobium japonicum Serogroup and Hydrogenase Phenotype Distribution in 12 States

Keyser

Weber

Uratsu

1984

Appl Environ Microbiol

View full text Add to dashboard Cite

A survey was conducted in 1980 on 972 isolates of Rhizobium japonicum obtained from 65 soybean field locations in 12 states. Isolates were examined for the hydrogenase (Hup) phenotype and somatic serogroup identity. Only 20% of the isolates were Hup+, with a majority of Hup-isolates occurring in 10 of the 12 states. The most predominant serogroup was 31 (21.5%), followed by 123 (13.6%). Although most serogroups contained a majority of Hup-isolates, marked differences occurred. None of the isolates in serogroup 135 were Hup+, but 93% of the isolates in serogroup 122 were Hup+. The serogroups with relatively high frequencies of Hup+ isolates (122 and 110) constitute only a small part (<5% each) of the R. japonicum field popuiation in the 12 states.

show abstract

mentioning

confidence: 99%

Rhizobium japonicum Serogroup and Hydrogenase Phenotype Distribution in 12 States

Keyser

Weber

Uratsu

1984

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…Enzyme systems that catalyze oxidation of H2 by 02 have been found in a limited number of Rhizobium spp. strains occurring primarily within the slow-growing taxonomic groups (12,18,23). In some of these H2 uptake-positive (Hup+) strains, sufficient H2-oxidizing activity is present to recycle all of the H2 produced during symbiotic N2 fixation.…”

mentioning

confidence: 99%

Characterization of Rhizobium japonicum hydrogen uptake genes

Haugland¹,

Cantrell²,

Beaty³

et al. 1984

J Bacteriol

View full text Add to dashboard Cite

Recombinant cosmids from a gene library of the DNA from Hup+ Rhizobium japonicum 122DES previously have been shown to restore hydrogenase activity when transferred by conjugation into certain Hup- mutants of R. japonicum. We generated a restriction map covering 32.2 kilobases of this cosmid DNA. At least 25.3 kilobases of the cosmid pHU1 were shown to have the same arrangement as those in the genome of strain 122DES. Analysis of Tn5 insertions into the 122DES genome indicates that hup-specific sequences occur in a region spanning about 15 kilobases of insert DNA within pHU1. Introduction of pHU1 into five out of six R. japonicum Hup- mutants resulted in a Hup+ phenotype in some transconjugants. Three of the mutations appear to be in transcriptional units completely contained within pHU1, whereas the other two must be in genes that are at least partially contained within pHU1. pBR235 derivatives containing fragments of hup DNA can be transferred into the R. japonicum Hup- mutant PJ18nal if the derivatives contain a region of homology with the R. japonicum genome. The hup mutation in strain PJ18nal appears to be dominant. The hup genes in R. japonicum strain 122DES appear to be organized in at least two, and probably three, transcriptional units.

show abstract

Hydrogenase and Energy Efficiency in Nitrogen Fixing Symbionts

Brewin

1984

Genes Involved in Microbe-Plant Interactions

View full text Add to dashboard Cite

Hydrogen Uptake (Hydrogenase) Activity of Rhizobium Japonicum Strains Forming Nodules in Soybean Production Areas of the U.S.A.

Cited by 7 publications

References 13 publications

Rhizobium japonicum Serogroup and Hydrogenase Phenotype Distribution in 12 States

Rhizobium japonicum Serogroup and Hydrogenase Phenotype Distribution in 12 States

Characterization of Rhizobium japonicum hydrogen uptake genes

Hydrogenase and Energy Efficiency in Nitrogen Fixing Symbionts

Contact Info

Product

Resources

About