Hydrogen sulfide-releasing silk fibroin scaffold for bone tissue engineering

Gambari, Laura; Amore, Emanuela; Raggio, Rosasilvia; Bonani, Walter; Barone, Marli; Lisignoli, Gina; Grigolo, Brunella; Motta, Antonella; Grassi, Francesco

doi:10.1016/j.msec.2019.04.039

Cited by 51 publications

(35 citation statements)

References 54 publications

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“…These three scaffolds showed protective effects on the oxidative damage of ROS and the ability to improve cell viability. A sponge H 2 S-releasing silk fibroin (SF) was also doped with GYY4137 [167], resulting in a scaffold with the same mechanical properties, which was able to induce a significant increase in differentiation to mature osteoblasts (OBs) and expression of osteogenic genes after three weeks of growth in a 3D culture of human MSCs. Table 2 summarizes scaffolds and H 2 S-releasing scaffolds that are currently under investigation for tissue repair.…”

Section: Tissue Regeneration As a Therapeutic Approach In Arthritismentioning

confidence: 99%

Hydrogen Sulfide as Potential Regulatory Gasotransmitter in Arthritic Diseases

Sunzini

Stefano

Chimenti

et al. 2020

IJMS

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The social and economic impact of chronic inflammatory diseases, such as arthritis, explains the growing interest of the research in this field. The antioxidant and anti-inflammatory properties of the endogenous gasotransmitter hydrogen sulfide (H 2 S) were recently demonstrated in the context of different inflammatory diseases. In particular, H 2 S is able to suppress the production of pro-inflammatory mediations by lymphocytes and innate immunity cells. Considering these biological effects of H 2 S, a potential role in the treatment of inflammatory arthritis, such as rheumatoid arthritis (RA), can be postulated. However, despite the growing interest in H 2 S, more evidence is needed to understand the pathophysiology and the potential of H 2 S as a therapeutic agent. Within this review, we provide an overview on H 2 S biological effects, on its role in immune-mediated inflammatory diseases, on H 2 S releasing drugs, and on systems of tissue repair and regeneration that are currently under investigation for potential therapeutic applications in arthritic diseases.

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Section: Tissue Regeneration As a Therapeutic Approach In Arthritismentioning

confidence: 99%

Hydrogen Sulfide as Potential Regulatory Gasotransmitter in Arthritic Diseases

Sunzini

Stefano

Chimenti

et al. 2020

IJMS

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“…Bone tissue engineering (BTE) refers to the high-concentration osteoblasts, bone marrow stromal stem cells or chondrocytes that are isolated from the organism itself and which are cultured in vitro and transplanted into a cell scaffold [132][133][134]. A perfect scaffold must be biodegradable and biocompatible, promote cell adhesion and proliferation, and preserve cell metabolism.…”

Section: Bone Tissue Engineering Materialsmentioning

confidence: 99%

Chitosan Derivatives and Their Application in Biomedicine

Wang

Meng

et al. 2020

IJMS

585

326

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Chitosan is a product of the deacetylation of chitin, which is widely found in nature. Chitosan is insoluble in water and most organic solvents, which seriously limits both its application scope and applicable fields. However, chitosan contains active functional groups that are liable to chemical reactions; thus, chitosan derivatives can be obtained through the chemical modification of chitosan. The modification of chitosan has been an important aspect of chitosan research, showing a better solubility, pH-sensitive targeting, an increased number of delivery systems, etc. This review summarizes the modification of chitosan by acylation, carboxylation, alkylation, and quaternization in order to improve the water solubility, pH sensitivity, and the targeting of chitosan derivatives. The applications of chitosan derivatives in the antibacterial, sustained slowly release, targeting, and delivery system fields are also described. Chitosan derivatives will have a large impact and show potential in biomedicine for the development of drugs in future.

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“…Indeed, a number of studies have established extensive crosstalk between H 2 S and VEGF. Exogenously administered H 2 S has been shown to upregulate VEGF expression in several tissues and cells [75], among which skeletal muscle cells and bone cells in vitro and in vivo [76,77]. H 2 S also significantly upregulated HIF-1alpha protein levels and increased HIF-1alpha binding activity under hypoxic conditions [78].…”

Section: Bone Tissue Repair: H2s As a Suitable Biological Cue For mentioning

confidence: 99%

“…Notably, H 2 S-deficiency due to CBS knock-down was linked to impaired osteogenesis and bone loss in mice [15]. Several pathways are implicated in H 2 S-dependent induction of osteogenesis, summarized in Figure 2: the WNT pathway [14,88]; BMP pathway [77]; and p38-MAPK and ERK signaling pathways [84,89]. Moreover, S-sulfhydration of cysteine residues in critical proteins involved in osteogenesis, such as Runx-2 [81] and Ca 2+ TRP channels [15], has been shown to be a mechanism of H 2 S-dependent induction of osteogenesis.…”

Section: Bone Tissue Repair: H2s As a Suitable Biological Cue For mentioning

confidence: 99%

“…The only reported H 2 S-releasing biomaterial targeting bone regeneration was developed by our group [77]. Based on previous preclinical studies showing efficient stimulation of osteogenesis by H 2 S donors, we postulated that a local release of physiologically relevant H 2 S concentrations may trigger osteogenic differentiation of h-MSCs.…”

Section: H2s-releasing Scaffolds For Regenerative Medicinementioning

confidence: 99%

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Hydrogen Sulfide in Bone Tissue Regeneration and Repair: State of the Art and New Perspectives

Gambari

Grigolo

Grassi

2019

IJMS

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The importance of hydrogen sulfide (H2S) in the regulation of multiple physiological functions has been clearly recognized in the over 20 years since it was first identified as a novel gasotransmitter. In bone tissue H2S exerts a cytoprotective effect and promotes bone formation. Just recently, the scientific community has begun to appreciate its role as a therapeutic agent in bone pathologies. Pharmacological administration of H2S achieved encouraging results in preclinical studies in the treatment of systemic bone diseases, such as osteoporosis; however, a local delivery of H2S at sites of bone damage may provide additional opportunities of treatment. Here, we highlight how H2S stimulates multiple signaling pathways involved in various stages of the processes of bone repair. Moreover, we discuss how material science and chemistry have recently developed biomaterials and H2S-donors with improved features, laying the ground for the development of H2S-releasing devices for bone regenerative medicine. This review is intended to give a state-of-the-art description of the pro-regenerative properties of H2S, with a focus on bone tissue, and to discuss the potential of H2S-releasing scaffolds as a support for bone repair.

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Hydrogen sulfide-releasing silk fibroin scaffold for bone tissue engineering

Cited by 51 publications

References 54 publications

Hydrogen Sulfide as Potential Regulatory Gasotransmitter in Arthritic Diseases

Hydrogen Sulfide as Potential Regulatory Gasotransmitter in Arthritic Diseases

Chitosan Derivatives and Their Application in Biomedicine

Hydrogen Sulfide in Bone Tissue Regeneration and Repair: State of the Art and New Perspectives

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