Hydrogen Sulfide-Releasing Fibrous Membranes: Potential Patches for Stimulating the Human Stem Cells Proliferation and Viability Under Oxidative Stress.

Abstract

The design of biomaterial platforms able to release bioactive molecules is mandatory in tissue repair and regenerative medicine. In this context, electrospinning is a user-friendly, versatile and low-cost technique, able to process different kinds of materials in micro- and nano-fibers with a large surface area-to-volume ratio for an optimal release of gaseous signalling molecules. Recently, the antioxidant and anti-inflammatory properties of the endogenous gasotramsmitter hydrogen sulfide (H2S), as well as its ability to stimulate relevant biochemical processes on the growth of mesenchymal stem cells (MSC), have been investigated. Therefore, in this work, new poly(lactic) acid fibrous membranes (PFM), doped and functionalized with H2S slow-releasing donors extracted from garlic, were synthetized. These innovative H2S-releasing mats were characterized for their morphological, thermal, mechanical and biological properties. Their antimicrobial activity and effects on the in vitro human cardiac MSC growth, either in the presence or in the absence of oxidative stress, were here assessed. On the basis of the results here presented, these new H2S-releasing PFM could represent promising and low-cost scaffolds or patches for biomedical applications in tissue repair.

Conductive Hydrogen Sulfide-Releasing Hydrogel Encapsulating ADSCs for Myocardial Infarction Treatment

“…It was demonstrated that H 2 S-releasing materials are able to enhance the proliferation of mesenchymal stem cells and cardiomyocytes (CMs). 52,53 To confirm the positive effects of H 2 Sreleasing hydrogel on treating MI, the expressions of inflammatory factors (TNF-α) and proangiogenic growth factors (VEGFA and Ang-1 mRNA) in the myocardium were determined by RT-PCR. As shown in Figure 8A, the ALG-CHO/ALG−TA−APTC/Geln/ADSCs hydrogel can dramatically reduce the expression of TNF-α and increase the expressions of VEGFA and Ang-1 compared with the MI group and other hydrogel groups (Figure 8B,C).…”

The Benefits of Macromolecular/Supramolecular Approaches in Hydrogen Sulfide Delivery: A Review of Polymeric and Self-Assembled Hydrogen Sulfide Donors

“…In 2018, Melino and coworkers reported electrospun microfibrous polylactic acid (PLA) membranes containing garlic-derived organosulfur compounds fabricated using two different approaches (13). In the first approach, garlic oil-soluble extract (GaOS) was directly coated onto the electrospun microfibers (*700 nm in diameter) to yield GaOS-coated PLA fiber membranes (PFM+GaOS).…”