Hydrogen sulfide regulates lung tissue-oxidized glutathione and total antioxidant capacity in hypoxic pulmonary hypertensive rats

Abstract

Aim: To investigate the modulatory effect of sudium hydrosulfide on lung tissue‐oxidized glutathione and total antioxidant capacity in the development of hypoxic pulmonary hypertension (HPH). Methods: After 21 d of hypoxia, the mean pulmonary artery pressure was measured by cardiac catheterization. The plasma H2S level and production of H2S in the lung tissues were determined by using a spectrophotometer. The lung homogenates were assayed for total antioxidant capacity (T‐AOC), superoxide dismutase (SOD), oxidized glutathione (GSSG), reduced glutathione and malonaldehyde by colorimetry. The mRNA level of SOD was analyzed by real‐time PCR, and the SOD expression was detected by Western blotting. Results: In the hypoxia group, the plasma H2S concentration and H2S production in the lung was significantly decreased compared with the control group (187.2±13.1 vs 299.6±12.4 μmol/L; 0.138±0.013 vs 0.289±0.036 nmol·mg−1·min−1, P<0.01). The administration of sodium hydrosulfide could reduce the mean pulmonary artery pressure by 31.2% compared with the hypoxia group (P<0.01). Treatment with sodium hydrosulfide decreased GSSG, and the T‐AOC level of the lung tissues was enhanced compared with the hypoxia group (P<0.05). There were no significant changes in the lung tissue SOD mRNA level, protein level, and its activity among the 3 groups. Conclusion: Oxidative stress occurred in the development of HPH and was accompanied by a decrease in the endogenous production of H2S in the lung tissues. H2S acted as an antioxidant during the oxidative stress of HPH partly as a result of the attenuated GSSG content.

Hydrogen sulfide ameliorates hyperhomocysteinemia-associated chronic renal failure

“…Along the same line, Wei et al (48) showed that severe oxidative stress was present in a model of hypoxic pulmonary hypertension and was accompanied by a decrease in the endogenous production of H 2 S in the lung tissue. H 2 S, however, acted as an antioxidant during this oxidative stress, which was a result of the attenuated GSSG content.…”

“…It is reported that H 2 S is involved in the regulation of vascular tone (24) and hypertension (52) and protects neuronal cells from oxidative stress by increasing the intracellular concentration of antioxidant and GSH (19,48). Recently, Tripatara et al (44) demonstrated that generation of endogenous H 2 S limits renal ischemia/reperfusion injury and dysfunction.…”

Exogenous Hydrogen Sulfide (H2S) Reduces Blood Pressure and Prevents the Progression of Diabetic Nephropathy in Spontaneously Hypertensive Rats

“…Alternatively, the increased plasma and urinary H 2 S levels in diabetic-treated rats suggests that exogenous H 2 S might have improved endothelial function due to its reducing oxidative stress property as it has been reported to be a potent antioxidant molecule. 51,52 Nevertheless, the above hypothesis deserves further verification.…”

Hydrogen Sulfide and the Vasculature: A Novel Vasculoprotective Entity and Regulator of Nitric Oxide Bioavailability?

“…It is possible that part of the vasculoprotective effects may be due to inhibition of platelet aggregation [96] [11,[23][24][25][26] [21,22,30,114] …”