Hydrogen Sulfide Production with a Microbial Consortium Isolated from Marine Sediments Offshore

Briones-Gallardo, Roberto; González-Muñoz, Muriel; García-Bautista, Itzá; Valdés-Lozano, David; Toledano-Thompson, Tanit; Polanco-Lugo, Erik; Rivera‐Madrid, Renata; Valdez-Ojeda, Ruby

doi:10.3390/jmse10030436

Cited by 6 publications

(4 citation statements)

References 42 publications

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“…In agreement with the 16S rRNA gene sequencing, the SRB community populating all the reservoirs, apart from R4, is dominated by members of the Clostridia class, over the Deltaproteobacteria class. These taxa were also recently found in oil fields, sulphate and CH 4 ‐rich groundwater from deep boreholes, and marine sediments tested for H 2 S production (Bomberg et al, 2015; Briones‐Gallardo et al, 2022; Rizzo et al, 2020; Varjani & Gnansounou, 2017).…”

Section: Resultsmentioning

confidence: 55%

“…Additionally, several taxa such as D. vulgaris and Desulfomicrobium species were found limited to R4, and, according to KEGG functional analysis, they display the whole set of genes for the reduction of sulphate to H 2 S. The occurrence of these microorganisms had been previously reported in marine sediments and deep aquifers used for natural gas storage (Briones-Gallardo et al, 2022;Ranchou-Peyruse et al, 2019).…”

Section: Reduction Of Sulphur Compoundsmentioning

confidence: 80%

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Biogeochemical characterization of four depleted gas reservoirs for conversion into underground hydrogen storage

Bassani,

Bellini,

Vizzarro

et al. 2023

Environmental Microbiology

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Depleted gas reservoirs are a valuable option for underground hydrogen storage (UHS). However, different classes of microorganisms, which are capable of using free H2 as a reducing agent for their metabolism, inhabit deep underground formations and can potentially affect the storage. This study integrates metagenomics based on Illumina‐NGS sequencing of bacterial and archaeal 16S rRNA and dsrB and mcrA functional genes to unveil the composition and the variability of indigenous microbial populations of four Italian depleted reservoirs. The obtained mcrA sequences allow us to implement the existing taxonomic database for mcrA gene sequences with newly classified sequences obtained from the Italian gas reservoirs. Moreover, the KEGG and COG predictive functional annotation was used to highlight the metabolic pathways potentially associated with hydrogenotrophic metabolisms. The analyses revealed the specificity of each reservoir microbial community, and taxonomic and functional data highlighted the presence of an enriched number of taxa, whose activity depends on both reservoir hydrochemical composition and nutrient availability, of potential relevance in the context of UHS. This study is the very first to address the profiling of the microbial population and allowed us to perform a preliminary assessment of UHS feasibility in Italy.

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Section: Resultsmentioning

confidence: 55%

Section: Reduction Of Sulphur Compoundsmentioning

confidence: 80%

Biogeochemical characterization of four depleted gas reservoirs for conversion into underground hydrogen storage

Bassani,

Bellini,

Vizzarro

et al. 2023

Environmental Microbiology

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“…Along with AB and HM, several studies report the presence of SRBs in different geological formations such as oil fields, sulfate and CH4-rich groundwater from deep boreholes, marine sediments, aquifers and salt mines ( Martini et al, 2005 ; Mochimaru et al, 2007 ; Morozova et al, 2011 ; Bomberg et al, 2015 ; Varjani and Gnansounou, 2017 ; Rusmanis et al, 2019 ; Rizzo et al, 2020 ; Briones-Gallardo et al, 2022 ). Although initially believed to rely only on H 2 for sulfur reduction, different studies characterized SRBs as capable of generating electrons from the oxidation of organic compounds including sugars, organic acids (i.e., scVFAs, acetate) and amino acids.…”

Section: Discussionmentioning

confidence: 99%

Investigating the activity of indigenous microbial communities from Italian depleted gas reservoirs and their possible impact on underground hydrogen storage

Bellini,

Vasile,

Bassani

et al. 2024

Front. Microbiol.

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H2 produced from renewable energies will play a central role in both greenhouse gas reduction and decarbonization by 2050. Nonetheless, to improve H2 diffusion and utilization as a fuel, large storage capacity systems are needed. Underground storage of natural gas in depleted reservoirs, aquifers and salt caverns is a well-established technology. However, new challenges arise when it comes to storing hydrogen due to the occurrence and activity of indigenous microbial populations in deep geological formations. In a previous study, four Italian natural gas reservoirs were characterized both from a hydro-chemical and microbiological point of view, and predictive functional analyses were carried out with the perspective of underground hydrogen storage (UHS). In the present work, formation waters from the same reservoirs were used as inoculant during batch cultivation tests to characterize microbial activity and its effects on different gas mixtures. Results evidence a predominant acidogenic/acetogenic activity, whilst methanogenic and sulfate reducing activity were only marginal for all tested inoculants. Furthermore, the microbial activation of tested samples is strongly influenced by nutrient availability. Obtained results were fitted and screened in a computational model which would allow deep insights in the study of microbial activity in the context of UHS.

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“…The strategies for enhancement mainly focused on supplementation with organic carbon, either as defined compounds such as lactate ( Luptáková et al, 2016 ), pyruvate ( Calabrese and Bissonnette, 1990 ), ethanol ( Martin et al, 2003 ) or as complex sources like manure, whey, sawdust and other industry byproducts ( Christensen et al, 1996 ; Castro et al, 1999 ; Chang et al, 2000 ; Hao et al, 2014 ; Sampaio et al, 2020 ). This was investigated both at field sites ( Koschorreck et al, 2007 ; McCullough et al, 2008 ) and in more controlled laboratory settings, using in some cases the microbial communities present in the metal-contaminated material ( Greets et al, 2006 ) or mixed external environmental samples as additional microbial seed material ( Barbosa et al, 2014 ; Briones-Gallardo et al, 2022 ). However, biosulfidogenesis has also been shown to establish naturally in anoxic parts of AMD environments, both in AMD sediments ( Sánchez-Andrea et al, 2012 ; Falagán et al, 2015 ) and in the deep part of the water column of several permanently stratified (meromictic) APLs ( Falagán et al, 2014 ; Sánchez-España et al, 2020a ; van der Graaf et al, 2020 ), where it was linked to the natural attenuation of extreme conditions.…”

Section: Introductionmentioning

confidence: 99%

Glycerol amendment enhances biosulfidogenesis in acid mine drainage-affected areas: An incubation column experiment

Ilin

Graaf

Yusta

et al. 2022

Front. Bioeng. Biotechnol.

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Microbial sulfate (SO42−) reduction in Acid Mine Drainage (AMD) environments can ameliorate the acidity and extreme metal concentrations by consumption of protons via the reduction of SO42− to hydrogen sulfide (H2S) and the concomitant precipitation of metals as metal sulfides. The activity of sulfate-reducing bacteria can be stimulated by the amendment of suitable organic carbon sources in these generally oligotrophic environments. Here, we used incubation columns (IC) as model systems to investigate the effect of glycerol amendment on the microbial community composition and its effect on the geochemistry of sediment and waters in AMD environments. The ICs were built with natural water and sediments from four distinct AMD-affected sites with different nutrient regimes: the oligotrophic Filón Centro and Guadiana acidic pit lakes, the Tintillo river (Huelva, Spain) and the eutrophic Brunita pit lake (Murcia, Spain). Physicochemical parameters were monitored during 18 months, and the microbial community composition was determined at the end of incubation through 16S rRNA gene amplicon sequencing. SEM-EDX analysis of sediments and suspended particulate matter was performed to investigate the microbially-induced mineral (neo)formation. Glycerol amendment strongly triggered biosulfidogenesis in all ICs, with pH increase and metal sulfide formation, but the effect was much more pronounced in the ICs from oligotrophic systems. Analysis of the microbial community composition at the end of the incubations showed that the SRB Desulfosporosinus was among the dominant taxa observed in all sulfidogenic columns, whereas the SRB Desulfurispora, Desulfovibrio and Acididesulfobacillus appeared to be more site-specific. Formation of Fe3+ and Al3+ (oxy)hydroxysulfates was observed during the initial phase of incubation together with increasing pH while formation of metal sulfides (predominantly, Zn, Fe and Cu sulfides) was observed after 1–5 months of incubation. Chemical analysis of the aqueous phase at the end of incubation showed almost complete removal of dissolved metals (Cu, Zn, Cd) in the amended ICs, while Fe and SO42− increased towards the water-sediment interface, likely as a result of the reductive dissolution of Fe(III) minerals enhanced by Fe-reducing bacteria. The combined geochemical and microbiological analyses further establish the link between biosulfidogenesis and natural attenuation through metal sulfide formation and proton consumption.

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Hydrogen Sulfide Production with a Microbial Consortium Isolated from Marine Sediments Offshore

Cited by 6 publications

References 42 publications

Biogeochemical characterization of four depleted gas reservoirs for conversion into underground hydrogen storage

Biogeochemical characterization of four depleted gas reservoirs for conversion into underground hydrogen storage

Investigating the activity of indigenous microbial communities from Italian depleted gas reservoirs and their possible impact on underground hydrogen storage

Glycerol amendment enhances biosulfidogenesis in acid mine drainage-affected areas: An incubation column experiment

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