Hydrogen sulfide prevents H2O2-induced senescence in human umbilical vein endothelial cells through SIRT1 activation

Suo, Rong; Zhao, Zhihui; Tang, Zhi‐Han; Zhong, Ren; Liu, Xing; Liu, Lu‐Shan; Zuo, Wangmeng; Tang, Chao-Ke; Wei, Dangheng; Jiang, Zhi‐Sheng

doi:10.3892/mmr.2013.1417

Cited by 79 publications

(59 citation statements)

References 36 publications

(34 reference statements)

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“…H 2 O 2 is a common reactive oxygen species that promotes free radical generation, which is able to enter and accumulate within cells, resulting in cellular damage (10). The reported concentrations of H 2 O 2 required to induce cell lesions varies widely in the literature (11)(12)(13). In the present study, 500 mmol/l H 2 O 2 was used, and the results showed that ~10% of the cells were in the DNA synthesis (S) or mitotic (G 2 ) phase of the cell cycle.…”

Section: Discussionmentioning

confidence: 65%

Protective effects of monosialotetrahexosylganglioside sodium on H2O2-induced human vascular endothelial cells

Zhao

et al. 2015

Experimental and Therapeutic Medicine

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Section: Discussionmentioning

confidence: 65%

Protective effects of monosialotetrahexosylganglioside sodium on H2O2-induced human vascular endothelial cells

Zhao

et al. 2015

Experimental and Therapeutic Medicine

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“…Recent studies demonstrated that senescence can be induced in many cell types under various conditions and treatments such as hypoxia, uremia, UVB, radiation, H 2 O 2 and oxidized low-density lipoprotein (59)(60)(61)(62)(63)(64). Previous studies indicated that β-galactosidase activity is a major characteristic found in senescence cells and is generally applied to senescence detection (65)(66)(67).…”

Section: Discussionmentioning

confidence: 99%

RC-6 ribonuclease induces caspase activation, cellular senescence and neuron-like morphology in NT2 embryonal carcinoma cells

et al. 2014

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Abstract.Frog ribonucleases have been demonstrated to have anticancer activities. However, whether RC-6 ribonuclease exerts anticancer activity on human embryonal carcinoma cells remains unclear. In the present study, RC-6 induced cytotoxicity in NT2 cells (a human embryonal carcinoma cell line) and our studies showed that RC-6 can exert anticancer effects and induce caspase-9 and -3 activities. Moreover, to date, there is no evidence that frog ribonuclease-induced cytotoxicity effects are related to cellular senescence. Therefore, our studies showed that RC-6 can increase p16 and p21 protein levels and induce cellular senescence in NT2 cells. Notably, similar to retinoic acid-differentiated NT2 cells, neuron-like morphology was found on some remaining live cells after RC-6 treatment. In conclusion, our study is the first to demonstrate that RC-6 can induce cytotoxic effects, caspase-9/-3 activities, cellular senescence and neuron-like morphology in NT2 cells.

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“…NaHS treatment results in the upregulation of both XOD and p67 (phox) levels but down-regulation of Mn-SOD expression [116], thereby slowing down cell aging. Another study shows that H 2 S delays HUVEC senescence and prevents H 2 O 2 -induced damage via sirtuin 1 activation [125]. In addition, H 2 Sreleasing drugs, NaHS and ACS6 (both at 10 µM), inhibit superoxide formation and gp91 (phox) (a catalytic subunit of NADPH oxidase) expression in porcine pulmonary arterial ECs by increasing cAMP levels and recruiting PKA upon the inhibition of PDE-5 [126].…”

Section: The Roles Of H 2 S and No In Oxidative Stressmentioning

confidence: 99%

“…4), whereas NaHS pretreatment reverses the changes in SOD activity and H 2 O 2 level [116]. Similarly, the expression levels of both xanthine oxidase (XOD) and subunits p67 (phox) of NADPH oxidase are increased in the senescent group relative to the fresh one, whereas manganese-superoxide dismutase (Mn-SOD) expression levels are decreased [125]. NaHS treatment results in the upregulation of both XOD and p67 (phox) levels but down-regulation of Mn-SOD expression [116], thereby slowing down cell aging.…”

Section: The Roles Of H 2 S and No In Oxidative Stressmentioning

confidence: 99%

Hydrogen Sulfide and Endothelial Dysfunction: Relationship with Nitric Oxide

Altaany¹,

Moccia²,

Munaron³

et al. 2014

CMC

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The endothelium is a cellular monolayer that lines the inner surface of blood vessels and plays a central role in the maintenance of cardiovascular homeostasis by controlling platelet aggregation, vascular tone, blood fluidity and fibrinolysis, adhesion and transmigration of inflammatory cells, and angiogenesis. Endothelial dysfunctions are associated with various cardiovascular diseases, including atherosclerosis, hypertension, myocardial infarction, and cardiovascular complications of diabetes. Numerous studies have established the antiinflammatory, anti-apoptotic, and anti-oxidant effects of hydrogen sulfide (H 2 S), the latest member to join the gasotransmitter family along with nitric oxide and carbon monoxide, on vascular endothelium. In addition, H 2 S may prime endothelial cells (ECs) toward angiogenesis and contribute to wound healing, aside to its well-known ability to relax vascular smooth muscle cells (VSMCs), thereby reducing blood pressure. Finally, H 2 S may inhibit VSMC proliferation and platelet aggregation. Consistently, a deficit in H 2 S homeostasis is involved in the pathogenesis of atherosclerosis and of hyperglycaemic endothelial injury. Therefore, the application of H 2 S-releasing drugs or using gene therapy to increase endogenous H 2 S level may help restore endothelial function and antagonize the progression of cardiovascular diseases. The present article reviews recent studies on the role of H 2 S in endothelial homeostasis, under both physiological and pathological conditions, and its putative therapeutic applications. Endothelial structure and functionThe endothelium lines the luminal surface of the entire vascular tree and the cardiac chambers, where it is termed endocardium. As a consequence, the endothelium presents a rather broad extension (300 to 1000 m 2 ) and weight (1.5 kg), achieving sizes comparable to other vital organs of the human body [1,2]. The endothelium can, therefore, be regarded as a real "organ spread across the organism" [3] and, as such, not only it is important for the regulation of local tissue functions, but also for maintaining the whole organism homeostasis [4]. Vascular endothelial cells (ECs) possess a polarized architecture: their luminal membrane is directly exposed to hematic constituents and circulating cells, while the basolateral surface is separated from surrounding tissues by a glycoprotein basement membrane -the sub-endothelial basement -which is formed by fibronectin, laminin, and collagen and is secreted by ECs themselves [2].Heterogeneity is, however, the hallmark of endothelium. ECs differ in morphology, function, and gene expression profile, so that even neighbouring cells from the same organ and blood vessel type exhibit distinct features [4]. For instance, vascular ECs may vary in size, shape, thickness and position of the nucleus. Albeit oriented along direction of blood flow, to attenuate the impact of shear stress forces on the vascular wall, microvascular ECs are rather thin (0.1 µm) and spindle-like, whilst their macrovascul...

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Hydrogen sulfide prevents H2O2-induced senescence in human umbilical vein endothelial cells through SIRT1 activation

Cited by 79 publications

References 36 publications

Protective effects of monosialotetrahexosylganglioside sodium on H2O2-induced human vascular endothelial cells

Protective effects of monosialotetrahexosylganglioside sodium on H2O2-induced human vascular endothelial cells

RC-6 ribonuclease induces caspase activation, cellular senescence and neuron-like morphology in NT2 embryonal carcinoma cells

Hydrogen Sulfide and Endothelial Dysfunction: Relationship with Nitric Oxide

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