Hydrogen Sulfide Prevents Advanced Glycation End-Products Induced Activation of the Epithelial Sodium Channel

Wang, Qiushi; Song, Bo; Jiang, Shuai; Chen, Liang; Chen, Xiao; Shi, Jing; Li, Xinyuan; Sun, Yingying; Wu, Ming‐Ming; Zhao, Daqing; Zhang, Zhiren; Ma, Heping

doi:10.1155/2015/976848

Cited by 16 publications

(17 citation statements)

References 36 publications

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“…Confocal and immunofluorescence technology were used to examine reactive oxygen species (ROS) in the cells, as previously described [19]. Briefly, HUVECs were cultured on glass coverslips.…”

Section: Methodsmentioning

confidence: 99%

Transient Receptor Potential Melastatin 4 (TRPM4) Contributes to High Salt Diet-Mediated Early-Stage Endothelial Injury

Ding

Ban

Liu

et al. 2017

Cell Physiol Biochem

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Background/Aims: The present study investigated whether the transient receptor potential melastatin 4 (TRPM4) channel plays a role in high salt diet (HSD)-induced endothelial injuries. Methods: Western blotting and immunofluorescence were used to examine TRPM4 expression in the mesenteric endothelium of Dahl salt-sensitive (SS) rats fed a HSD. The MTT, TUNEL, and transwell assays were used to evaluate the cell viability, cell apoptosis, and cell migration, respectively, of human umbilical vein endothelial cells (HUVECs). Enzyme-linked immunosorbent assays were used to determine the concentrations of intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion protein 1 (VCAM-1), and E-selectin. Carboxy-H2DCFDA, a membrane-permeable reactive oxygen species (ROS)-sensitive fluorescent probe, was used to detect intracellular ROS levels. Results: TRPM4 was mainly expressed near the plasma membrane of mesenteric artery endothelial cells, and its expression level increased in SS hypertensive rats fed a HSD. Its protein expression was significantly upregulated upon treatment with exogenous hydrogen peroxide (H₂O₂) and aldosterone in cultured HUVECs. Cell viability decreased upon treatment with both agents in a concentration-dependent manner, which could be partially reversed by 9-phenanthrol, a specific TRPM4 inhibitor. Exogenous H₂O₂ induced apoptosis, enhanced cell migration, and increased the release of adhesion molecules, including ICAM-1, VCAM-1, and E-selectin, all of which were significantly attenuated upon treatment with 9-phenanthrol. Aldosterone and H₂O₂ induced the accumulation of intracellular ROS, which was significantly inhibited by 9-phenanthrol, suggesting that oxidative stress is one of the mechanisms underlying aldosterone-induced endothelial injury. Conclusions: Given the fact that oxidative stress and high levels of circulating aldosterone are present in hypertensive patients, we suggest that the upregulation of TRPM4 in the vascular endothelium may be involved in endothelial injuries caused by these stimuli.

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Section: Methodsmentioning

confidence: 99%

Transient Receptor Potential Melastatin 4 (TRPM4) Contributes to High Salt Diet-Mediated Early-Stage Endothelial Injury

Ding

Ban

Liu

et al. 2017

Cell Physiol Biochem

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“…Patch-clamp recording. Single-channel currents were recorded from mpkCCDc 14 cells under the voltage-clamp mode with an Axopatch-200B amplifier (Molecular Devices, Sunnyvale, CA), using the cell-attached and excised inside-out patch-clamp configurations, as described previously (37,43). Data were acquired and sampled with a low-pass, 1-kHz, eight-pole Bessel filter using a Digidata 1440A analog-digital interface (Axon Instruments).…”

Section: Methodsmentioning

confidence: 99%

Hydrogen peroxide suppresses TRPM4 trafficking to the apical membrane in mouse cortical collecting duct principal cells

Zhai

et al. 2016

American Journal of Physiology-Renal Physiology

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A Ca-activated nonselective cation channel (NSC) is found in principal cells of the mouse cortical collecting duct (CCD). However, the molecular identity of this channel remains unclear. We used mpkCCD cells, a mouse CCD principal cell line, to determine whether NSC represents the transient receptor potential (TRP) channel, the melastatin subfamily 4 (TRPM4). A Ca-sensitive single-channel current was observed in inside-out patches excised from the apical membrane of mpkCCD cells. Like TRPM4 channels found in other cell types, this channel has an equal permeability for Na and K and has a linear current-voltage relationship with a slope conductance of ~23 pS. The channel was inhibited by a specific TRPM4 inhibitor, 9-phenanthrol. Moreover, the frequency of observing this channel was dramatically decreased in TRPM4 knockdown mpkCCD cells. Unlike those previously reported in other cell types, the TRPM4 in mpkCCD cells was unable to be activated by hydrogen peroxide (HO). Conversely, after treatment with HO, TRPM4 density in the apical membrane of mpkCCD cells was significantly decreased. The channel in intact cell-attached patches was activated by ionomycin (a Ca ionophore), but not by ATP (a purinergic P receptor agonist). These data suggest that the NSC current previously described in CCD principal cells is actually carried through TRPM4 channels. However, the physiological role of this channel in the CCD remains to be further determined.

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“…An overproduction of H 2 O 2 , in addition to affecting the ability of CAT to metabolize, can cause damage to this enzyme, leading to a decrease in its activity (Omotayo et al, 2010). Another possibility is that CAT activity might be inhibited by advanced glycation end products, as described by Wang et al (2015).…”

Section: F I G U R Ementioning

confidence: 99%

Jabuticaba (Plinia trunciflora(O. Berg) Kausel) improved the lipid profile and immune system and reduced oxidative stress in streptozotocin‐induced diabetic rats

et al. 2020

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The aim of this study was evaluating the effects of jabuticaba aqueous extract (JPE ‐ 0.5 g/kg) on serum lipid levels, immune system, and oxidative stress parameters of streptozotocin‐induced diabetic rats. Administration of JPE for 30 days, by gavage, was able to reduce serum levels of total cholesterol, non‐high density lipoprotein (HDL) cholesterol, and triglycerides in diabetic rats. The HDL cholesterol levels increased in both diabetic and healthy rats after JPE treatment. Total leukocyte and lymphocyte counts reduced in diabetic rats, and JPE treatment prevented these diabetes mellitus (DM)‐induced changes in the immune system. In addition, the induction of DM also led to dysregulation in the activity of superoxide dismutase and catalase antioxidant enzymes as well as an increase in oxidative stress markers. Treatments with JPE reduced oxidative stress and modulated antioxidant enzyme activities. These data demonstrate the potential of JPE as an adjuvant treatment option for diabetic patients. Practical applications Considering that it is very common to observe dyslipidemia in diabetic patients and that these alterations, combined with the increased oxidative stress levels, also common in these patients, can lead to the development of cardiovascular diseases, JPE would be an alternative treatment adjunct to reduce these risks. In addition, although more studies are needed, JPE has the potential to improve the count of total lymphocytes and leukocytes, which could assist in improving the immune response of these patients, who also commonly have a higher risk of infectious diseases. Thus, JPE could be used by these patients, in combination with conventional treatment, in the form of a nutraceutical rich in phenolic compounds.

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Hydrogen Sulfide Prevents Advanced Glycation End-Products Induced Activation of the Epithelial Sodium Channel

Cited by 16 publications

References 36 publications

Transient Receptor Potential Melastatin 4 (TRPM4) Contributes to High Salt Diet-Mediated Early-Stage Endothelial Injury

Transient Receptor Potential Melastatin 4 (TRPM4) Contributes to High Salt Diet-Mediated Early-Stage Endothelial Injury

Hydrogen peroxide suppresses TRPM4 trafficking to the apical membrane in mouse cortical collecting duct principal cells

Jabuticaba (Plinia trunciflora(O. Berg) Kausel) improved the lipid profile and immune system and reduced oxidative stress in streptozotocin‐induced diabetic rats

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