Hydrogen sulfide modulates actin-dependent auxin transport via regulating ABPs results in changing of root development in Arabidopsis

Abstract

Hydrogen sulfide (H2S) signaling has been considered a key regulator of plant developmental processes and defenses. In this study, we demonstrate that high levels of H2S inhibit auxin transport and lead to alterations in root system development. H2S inhibits auxin transport by altering the polar subcellular distribution of PIN proteins. The vesicle trafficking and distribution of the PIN proteins are an actin-dependent process. H2S changes the expression of several actin-binding proteins (ABPs) and decreases the occupancy percentage of F-actin bundles in the Arabidopsis roots. We observed the effects of H2S on F-actin in T-DNA insertion mutants of cpa, cpb and prf3, indicating that the effects of H2S on F-actin are partially removed in the mutant plants. Thus, these data imply that the ABPs act as downstream effectors of the H2S signal and thereby regulate the assembly and depolymerization of F-actin in root cells. Taken together, our data suggest that the existence of a tightly regulated intertwined signaling network between auxin, H2S and actin that controls root system development. In the proposed process, H2S plays an important role in modulating auxin transport by an actin-dependent method, which results in alterations in root development in Arabidopsis.

Hydrogen Sulfide Increases Production of NADPH Oxidase-Dependent Hydrogen Peroxide and Phospholipase D-Derived Phosphatidic Acid in Guard Cell Signaling

“…Another explanation is related to the organization of the guard cell cytoskeleton, a key structure for the modulation of stomatal movement (Higaki et al, 2010). Given that PLDd modulates the cytoskeleton by forming physical bridges between the microtubules and the plasma membrane (Andreeva et al, 2009;Ho et al, 2009), and that H 2 S regulates microtubule organization in root hairs (Jia et al, 2015), it can be postulated that PLDd-derived PA might affect stomatal closure in response to H 2 S via modulation of microtubule organization.…”

Effects of discrete bioactive microbial volatiles on plants and fungi

“…These toxic effects of H 2 S emerged at high concentrations (ppm range). Because most likely such high levels are not emitted under natural conditions, H 2 S was recently considered in planta to be a signalling compound (Lisjak et al ), playing a role in stomatal apertures and improvement of drought resistance (Garcia‐Mata and Lamattina ; Jin et al ), controlling root system development (Jia et al ), altering enzyme activities and influencing NO and H 2 O 2 metabolism (Lisjak et al ). Thompson and Kats () even showed that low concentrations of H 2 S positively affect the growth of Medicago , lettuce and sugar beets.…”

Improving Photosynthetic Capacity, Alleviating Photosynthetic Inhibition and Oxidative Stress Under Low Temperature Stress With Exogenous Hydrogen Sulfide in Blueberry Seedlings

“…A high concentration of H 2 S can cause cytotoxicity, while a low concentration of H 2 S does not cause toxicity to plants and may act as a signaling molecule (Duan et al, 2015;Li et al, 2016). Recently, many studies have found that H 2 S can regulate plant growth and development, such as inducing plant seed germination (Liu and Lal, 2015), improving photosynthetic capacity (Coyne and Bingham, 1978;Chen et al, 2011), regulating stomatal movement (Lisjak et al, 2011;Scuffi et al, 2014), promoting the development of lateral roots (Jia et al, 2015;, regulating secondary metabolism of sugar, polyamines, organic acids and amino acids (Shi et al, 2015;Chen et al, 2016), participating in protein modification (Mustafa et al, 2009), maintaining ion balance in plants (Wang et al, 2012;Lai et al, 2014), delaying ripening and senescence of postharvest fruits during storage (Fu et al, 2014;Hu et al, 2014), and improving antioxidant capacity (Luo et al, 2015). In addition, H 2 S has been shown to participate in the regulation of resistance (Hua et al, 2010;Christou, 2013;Jin et al, 2018).…”