Hydrogen sulfide donor sodium hydrosulfide-induced heat tolerance in tobacco (Nicotiana tabacum L) suspension cultured cells and involvement of Ca2+ and calmodulin

Li, Zhong-Guang; Gong, Ming; Xie, Hong; Yang, Lan; Li, Jing

doi:10.1016/j.plantsci.2011.10.006

Cited by 185 publications

(129 citation statements)

References 31 publications

Supporting

Mentioning

122

Contrasting

Unclassified

Order By: Relevance

“…Recent studies in plants revealed that H 2 S plays multiple roles in the modulation of various physiological processes [3], such as copper, cadmium, drought, salinity and heat et al [6][7][8][9][10]. In addition, our results indicate that the content of H 2 S was rapidly increased by low temperature.…”

Section: Discussionsupporting

confidence: 56%

“…Many data indicate that most of the endogenously synthesized H 2 S occurred via L-cysteine desulfhydrase (LCD, EC4.4.1.1) and D-cysteine desulfhydrase (DCD, EC4.4.1.15) in high plants [2][3][4][5]. Recent studies show that H 2 S is involved not only in plant responses to drought and copper stresses but also in tolerance to salinity, heat, cadmium, boron and chromium stresses [6][7][8][9][10]. It is reported that exogenous H 2 S can enhance the resistance of plants to drought stress [11][12][13][14] and copper stress [15,16] by improving the activities of antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Hydrogen sulfide is involved in the chilling stress response in Vitis vinifera L.

et al. 2013

View full text Add to dashboard Cite

Hydrogen sulfide (H 2 S) is an important signaling molecule involved in several stress-resistance processes in plants, such as drought and heavy metal stresses. However, little is known about the roles of H 2 S in responses to chilling stress. In this paper, we demonstrated that chilling stress enhance the H 2 S levels, the H 2 S synthetase (L-/D-cysteine desulfhydrase, L/DCD) activities, and the expression of L/DCD gene in Vitis vinifera L. 'F-242' . Furthermore, the seedlings were treated with sodium hydrosulfide (NaHS, a H 2 S donor) and hypotaurine (HT, a H 2 S scavenger) at 4°C to examine the effects of exogenous H 2 S on grape. The results revealed that the high activity of superoxide dismutase and enhanced expression of VvICE1 and VvCBF3 genes, but low level of superoxide anion radical, malondialdehyde content and cell membrane permeability were detected after addition of NaHS. In contrast, HT treatment displayed contrary effect under the chilling temperature. Taken together, these data suggested that H 2 S might be directly involved in the cold signal transduction pathway of grape.

show abstract

Section: Discussionsupporting

confidence: 56%

Section: Introductionmentioning

confidence: 99%

Hydrogen sulfide is involved in the chilling stress response in Vitis vinifera L.

et al. 2013

View full text Add to dashboard Cite

show abstract

“…The novel functions of hydrogen sulfide have been uncovered in seed germination (Zhang et al 2010a;Li et al 2012a), root organogenesis (Zhang et al 2009a) and stomata movement (Lisjak et al 2010;Liu et al 2011;Jin et al 2013), as well as in alleviation in osmotic stress (Zhang et al 2009b), salt stress (Wang et al 2012), oxidative stress (Shan et al 2011, heavy metal stress (Zhang et al 2008(Zhang et al , 2010bChen et al 2013) and pathogen infection (Bloem et al 2011). Our previous results also showed that pretreatment with sodium hydrosulfide (NaHS), a hydrogen sulfide donor, could improve resistance of tobacco cells (Li et al 2012b) and maize seedlings (Li et al 2013a(Li et al ,b, 2014 to high temperature, but its mechanism is not fully clear. In this study, effect of NaHS pretreatment on heat tolerance of maize seedlings in relation to antioxidant system was investigated.…”

Section: Introductionmentioning

confidence: 99%

Effect of pretreatment with hydrogen sulfide donor sodium hydrosulfide on heat tolerance in relation to antioxidant system in maize (Zea mays) seedlings

2014

Biologia

Self Cite

View full text Add to dashboard Cite

Hydrogen sulfide (H2S) is a signal molecule that is involved in plant growth, development and the acquisition of stress tolerance including heat tolerance, but the mechanism of H2S-induced heat tolerance is not completely clear. In present study, the effect of sodium hydrosulfide (NaHS), a H2S donor, treatment on heat tolerance of maize seedlings in relation to antioxidant system was investigated. The results showed that NaHS treatment improved survival percentage of maize seedlings under heat stress in a concentration-dependent manner, indicating that H2S treatment could improve heat tolerance of maize seedlings. To further study mechanism of NaHS-induced heat tolerance, catalase (CAT), guaiacol peroxidase (GPX), superoxide dismutase (SOD), glutathione reductase (GR) and ascorbate peroxidase (APX) activities, and glutathione (GSH) and ascorbic acid (AsA) contents in maize seedlings were determined. The results showed that NaHS treatment increased the activities of CAT, GPX, SOD and GR, and GSH and AsA contents as well as the ratio of reduced antioxidants to total antioxidants [AsA/(AsA+DHA) and GSH/(GSH +GSSG)] in maize seedlings under normal culture conditions compared with the control. Under heat stress, antioxidant enzymes activities, antioxidants contents and the ratio of the reduced antioxidants to total antioxidants in control and treated seedlings all decreased, but NaHS-treated seedlings maintained higher antioxidant enzymes activities and antioxidants levels as well as the ratio of reduced antioxidants to total antioxidants. All of above-mentioned results suggested that NaHS treatment could improve heat tolerance of maize seedlings, and the acquisition of this heat tolerance may be relation to enhanced antioxidant system activity.

show abstract

“…Similarly, the inhibitory effect of boron on cucumber (Cucumis sativus) root elongation is substantially reduced by H 2 S treatment, which upregulates the cell wall-associated proteins and expansins . Moreover, sodium hydrosulfide pretreatment improves heat tolerance in tobacco (Nicotiana tabacum) suspension cultured cells, requiring the involvement of calcium and calmodulin (Li et al, 2012). It has also been reported that sulfur fertilization increases plant resistance against fungal pathogens, and it has been proposed that H 2 S is involved in the mechanisms of the sulfur-induced resistance, or sulfur-enhanced defense, phenomenon (Rausch and Wachter, 2005).…”

Section: Introductionmentioning

confidence: 99%

Cysteine-Generated Sulfide in the Cytosol Negatively Regulates Autophagy and Modulates the Transcriptional Profile in Arabidopsis

et al. 2012

View full text Add to dashboard Cite

In Arabidopsis thaliana, DES1 is the only identified L-Cysteine desulfhydrase located in the cytosol, and it is involved in the degradation of cysteine and the concomitant production of H 2 S in this cell compartment. Detailed characterization of the T-DNA insertion mutants des1-1 and des1-2 has provided insight into the role of sulfide metabolically generated in the cytosol as a signaling molecule. Mutations of L-CYS DESULFHYDRASE 1 (DES1) impede H 2 S generation in the Arabidopsis cytosol and strongly affect plant metabolism. Senescence-associated vacuoles are detected in mesophyll protoplasts of des1 mutants. Additionally, DES1 deficiency promotes the accumulation and lipidation of the ATG8 protein, which is associated with the process of autophagy. The transcriptional profile of the des1-1 mutant corresponds to its premature senescence and autophagy-induction phenotypes, and restoring H 2 S generation has been shown to eliminate the phenotypic defects of des1 mutants. Moreover, sulfide is able to reverse ATG8 accumulation and lipidation, even in wild-type plants when autophagy is induced by carbon starvation, suggesting a general effect of sulfide on autophagy regulation that is unrelated to sulfur or nitrogen limitation stress. Our results suggest that cysteine-generated sulfide in the cytosol negatively regulates autophagy and modulates the transcriptional profile of Arabidopsis.

show abstract

Hydrogen sulfide donor sodium hydrosulfide-induced heat tolerance in tobacco (Nicotiana tabacum L) suspension cultured cells and involvement of Ca2+ and calmodulin

Cited by 185 publications

References 31 publications

Hydrogen sulfide is involved in the chilling stress response in Vitis vinifera L.

Hydrogen sulfide is involved in the chilling stress response in Vitis vinifera L.

Effect of pretreatment with hydrogen sulfide donor sodium hydrosulfide on heat tolerance in relation to antioxidant system in maize (Zea mays) seedlings

Cysteine-Generated Sulfide in the Cytosol Negatively Regulates Autophagy and Modulates the Transcriptional Profile in Arabidopsis

Contact Info

Product

Resources

About