Hydrogen Sulfide Attenuates Ischemia-Reperfusion Injury in In Vitro and In Vivo Models of Intestine Free Tissue Transfer

Abstract

Hydrogen sulfide significantly attenuates ischemia-reperfusion injury in intestinal tissue in vitro and in vivo. These results have significant implications for enteric free tissue transfers and other gastrointestinal procedures in which ischemic intervals may be anticipated.

Protective Effects of Exogenous Hydrogen Sulfide on Neurons of Hippocampus in a Rat Model of Brain Ischemia

“…*P \ 0.05, comparison between 2VO group and 2VO-NaHS group. # P \ 0.05, comparison between sham group and 2VO group production may be of therapeutic benefit in the treatment of ischemia-induced organ injury [3,[39][40][41]. However, less investigation was about the effect of H 2 S on brain ischemia in vivo.…”

“…H 2 S-mediated cytoprotection was in connection with an improved balance between reduced GSH and oxidized glutathione (GSSG), an attenuated formation of lipid hydroperoxides, and an increased expression of thioredoxin-1 (Trx-1) [17]. Previous studies demonstrated that H 2 S significantly decreased apoptosis in vivo and vitro in the setting of ischemia injury [27,39]. H 2 S inhibited the progression of apoptosis after I/R injury by increasing the protein expression of heat shock protein (HSP-90) and Bcl-2 [17].…”

The Effects of Hydrogen Sulfide on Adipocyte Viability in Human Adipocyte and Adipocyte-Derived Mesenchymal Stem Cell Cultures Under Ischemic Conditions

“…Moreover, we found that the protective effect of NaHS is less evident at higher doses than at lower doses, as seen in previous studies. [15][16][17] According to our results, we propose that the most beneficial NaHS concentrations are 0.1 to 100 μM for adipose tissue.…”

“…For in vitro cell culture studies for hypoxia, 1% O 2 levels have been used. 16,17 Similar to the literature, the hypoxic conditions in our study were set to 1% O 2 for the mature adipocyte and adipose-derived mesenchymal cell cultures. By using a special incubator, the gas levels could be set and maintained at constant levels during the experiment.…”

“…13 Hydrogen sulfide has emerged as a potent cytoprotective mediator in various models of tissue and cellular injuries but there is no research about its effects on adipose tissue in the literature. [14][15][16][17] Hydrogen sulfide increases resistance to ischemia in some tissues and decreases apoptosis; however, the exact mechanism is still unclear.…”