Hydrogen sulfide alleviates toxic effects of arsenate in pea seedlings through up-regulation of the ascorbate–glutathione cycle: Possible involvement of nitric oxide

Singh, Vijay Pratap; Singh, Samiksha; Kumar, J.; Prasad, Sheo Mohan

doi:10.1016/j.jplph.2015.03.015

Cited by 223 publications

(98 citation statements)

References 70 publications

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“…Meanwhile, higher cysteine level was observed in As-stress seedlings in comparison to all other treatments (As-free; NaHS; As + NaHS), while these effects were alleviated by the addition of NaHS (Singh et al, 2015). Further experiments showed that As treatment inhibited the activity of the enzymes involved in the ascorbic acid (AsA)–glutathione (GSH) cycle, whereas their activities were enhanced by application of NaHS (Singh et al, 2015). In addition, the redox status of AsA and GSH was disturbed, as indicated by a steep decline in their reduced/oxidized ratios.…”

Section: H2s-induced Cross-adaptationmentioning

confidence: 93%

“…In addition, the redox status of AsA and GSH was disturbed, as indicated by a steep decline in their reduced/oxidized ratios. However, exogenously applied NaHS restored the redox status of the AsA and GSH pools under As stress (Singh et al, 2015). Furthermore, NaHS treatment ameliorated As toxicity, which was coincided with the increased accumulation of H 2 S. The results demonstrated that H 2 S might counterbalance ROS-mediated damage to macromolecules by reducing the accumulation of As and triggering up-regulation of the AsA–GSH cycle, further suggesting that H 2 S plays a crucial role in plant priming, and in particular for pea seedlings in mitigating As stress.…”

Section: H2s-induced Cross-adaptationmentioning

confidence: 99%

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Hydrogen Sulfide: A Signal Molecule in Plant Cross-Adaptation

2016

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For a long time, hydrogen sulfide (H2S) has been considered as merely a toxic by product of cell metabolism, but nowadays is emerging as a novel gaseous signal molecule, which participates in seed germination, plant growth and development, as well as the acquisition of stress tolerance including cross-adaptation in plants. Cross-adaptation, widely existing in nature, is the phenomenon in which plants expose to a moderate stress can induce the resistance to other stresses. The mechanism of cross-adaptation is involved in a complex signal network consisting of many second messengers such as Ca2+, abscisic acid, hydrogen peroxide and nitric oxide, as well as their crosstalk. The cross-adaptation signaling is commonly triggered by moderate environmental stress or exogenous application of signal molecules or their donors, which in turn induces cross-adaptation by enhancing antioxidant system activity, accumulating osmolytes, synthesizing heat shock proteins, as well as maintaining ion and nutrient balance. In this review, based on the current knowledge on H2S and cross-adaptation in plant biology, H2S homeostasis in plant cells under normal growth conditions; H2S signaling triggered by abiotic stress; and H2S-induced cross-adaptation to heavy metal, salt, drought, cold, heat, and flooding stress were summarized, and concluded that H2S might be a candidate signal molecule in plant cross-adaptation. In addition, future research direction also has been proposed.

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Section: H2s-induced Cross-adaptationmentioning

confidence: 93%

Section: H2s-induced Cross-adaptationmentioning

confidence: 99%

Hydrogen Sulfide: A Signal Molecule in Plant Cross-Adaptation

2016

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“…Oxidative stress induced by As V (25 and 50 µM) in maize cultivar and hybrid were further correlated with the redox status of ascorbate (AsA+DHA, AsA, and DHA) and glutathione (GSH+GSSG, GSH, and GSSG). It has been shown that ascorbate (AsA) plays an important role in scavenging ROS directly and indirectly (Foyer and Noctor, 2011;Singh et al, 2015a). Furthermore, the protective role of ascorbate (AsA) against oxidative stress has been demonstrated in Arabidopsis ascorbate-deficient (vtc1) mutant (Gao and Zhang, 2008).…”

Section: Treatmentsmentioning

confidence: 99%

“…In last two decades, millions of people are exposed to arsenic (As) poisoning due to drinking of As-contaminated water (Nordstrom, 2002;Patel et al, 2005;Zhao et al, 2009;Singh R. et al, 2015;Singh et al, 2015a). Arsenic is found in different oxidation states, with the most dominant forms being trivalent (As III ) and penta-valent (As V ), which are classified as potent carcinogens Abbreviations: AsA, reduced ascorbate; APX, ascorbate peroxidase; DHA, dehydroascorbate; DHAR, dehydrateascorbate reductase; F v /F m , maximum photochemical efficiency of PS II; GR, glutathione reductase; GSH, reduced glutathione; GSSG, oxidized glutathione; H 2 O 2 , hydrogen peroxide; MDA, malondialdehyde; NPQ, non-photochemical quenching; qP, photochemical quenching; ROS, reactive oxygen species; Si, silicon; SiNp, silicon nanoparticle; SOD, superoxide dismutase; SOR, superoxide radical.…”

Section: Introductionmentioning

confidence: 99%

Silicon Nanoparticles More Efficiently Alleviate Arsenate Toxicity than Silicon in Maize Cultiver and Hybrid Differing in Arsenate Tolerance

Tripathi

Singh

et al. 2016

Front. Environ. Sci.

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277

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Alleviation of abiotic stress by silicon (Si) is well documented. But mitigation of abiotic stress by silicon nanoparticles (SiNPs) is not much known. Therefore, hydroponic experiments were conducted to investigate if SiNPs are more effective than Si in mitigation of arsenate (As V ; 25 and 50 µM) toxicity in maize cultivar and hybrid differing in As V tolerance. Under As V stress, reduction in growth was accompanied by enhanced level of As and oxidative stress. As V inhibited activities of antioxidant enzymes like ascorbate peroxidase, glutathione reductase, and dehydroascorbate reductase (except superoxide dismutase). The redox status of ascorbate and glutathione was disturbed by As V as indicated by a steep decline in their reduced/oxidized ratios. However, addition of Si and SiNp ameliorates As V toxicity in maize. Si and SiNp both could reduce As V toxicity in maize cultivar and hybrid, which could be related with decreased accumulation of As and oxidative stress, and enhanced components of the ascorbate-glutathione cycle (AsA-GSH cycle). But lowering in the accumulation of As and oxidative stress markers, and enhancement in components of the AsA-GSH cycle was prominent in SiNp fed seedlings under As V stress. The results also showed that SiNp are more effective in reducing As V toxicity than Si, which is due to their greater availability to seedlings. Comparing responses of cultivar and hybrid, maize cultivar shows more resistance against As V than hybrid.

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“…For the first time, using the SOD inhibitor DDC, the value of this enzyme in the formation of hydrogen peroxide signal pool, necessary for activating antioxidant enzymes and improving the heat resistance of coleoptiles, has been shown. In the future, it seems relevant to clarify the question of the functional interaction of hydrogen sulfide with other signal media tors, primarily calcium ions and nitric oxide, which have close functional links with ROS [6,31,32].…”

Section: Fig 3 Content Of Hydrogen Peroxide In Wheat Coleoptiles Unmentioning

confidence: 99%

nduction of plant cells heat resistance by hydrogen sulfide donor is mediated by H(2)O(2) generation with participation of NADPH oxidase and superoxide dismutase

Kolupaev¹,

Firsova²,

Yastreb³

2017

Ukr.Biochem.J.

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A t present, it is known that the formation of plant resistance to stress factors occurs with the participation of a number of signal mediators. The role of calcium ions, reactive oxygen species (ROS) and nitric oxide in transduction of stress signals into genetic apparatus of a plant cell and formation of adaptive reactions is most studied [1][2][3].In recent years, hydrogen sulfide (H 2 S) has been considered as another inorganic mediating molecule both in animals [4] and in plant cells [5,6].Effects of endogenous hydrogen sulfide content increasing in plants under action of stressors, in particular, drought [7], salinity [8], heavy metals [9] were shown. An increase in content of hydrogen sulfide at treatment of maize seedlings with NO donors or hydrogen peroxide inducing heat resistance was revealed [10,11]. This indicates a close relationship between hydrogen sulfide, ROS and NO as signa ling molecules.Data have also been obtained that indicate the role of ROS in transduction of H 2 S signal. Increasing resistance of barley plants to UV-B by hydrogen sulfide donor was accompanied by an increase in content of hydrogen peroxide in leaves and this effect was eliminated by the H 2 O 2 scavenger dimethylthiourea [12]. We have previously shown that induced by the hydrogen sulfide donor NaHS increase in heat resistance of wheat coleoptiles was leveled by treatment with an antioxidant ionol [13]. At the same time, however, the role of ROS in realization of the effects of hydrogen sulfide as a physiologically active

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Hydrogen sulfide alleviates toxic effects of arsenate in pea seedlings through up-regulation of the ascorbate–glutathione cycle: Possible involvement of nitric oxide

Cited by 223 publications

References 70 publications

Hydrogen Sulfide: A Signal Molecule in Plant Cross-Adaptation

Hydrogen Sulfide: A Signal Molecule in Plant Cross-Adaptation

Silicon Nanoparticles More Efficiently Alleviate Arsenate Toxicity than Silicon in Maize Cultiver and Hybrid Differing in Arsenate Tolerance

nduction of plant cells heat resistance by hydrogen sulfide donor is mediated by H(2)O(2) generation with participation of NADPH oxidase and superoxide dismutase

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