Hydrogen sulfide, a novel small molecule signalling agent, participates in the regulation of ganoderic acids biosynthesis induced by heat stress in Ganoderma lucidum

Tian, Jia-Long; Ren, Ang; Wang, Ting; Zhu, Jing; Hu, Yanru; Shi, Liang; Yu, Hongyi; Zhao, Mingwen

doi:10.1016/j.fgb.2019.04.014

Cited by 28 publications

(29 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Kong et al (2012) discovered that nitric oxide (NO) can alleviate heat stress-induced oxidative damage in Pleurotus eryngii. Other signaling molecules similar to NO, such as calcium (Ca 2+ ), reactive oxygen species (ROS), and hydrogen sulfide (H 2 S), were found to participate in the heat stress response in G. lucidum (Zhang et al, 2016;Liu et al, 2018b;Tian et al, 2019). Finally, further studies have reported activated phospholipase D (PLD), a significant accumulation of phosphatidic acid (PA) (Liu et al, 2017a), and increased cell membrane fluidity upon heat stress (Liu et al, 2017b).…”

Section: Introductionmentioning

confidence: 99%

Metabolic Response of Pleurotus ostreatus to Continuous Heat Stress

Yan

Zhao

et al. 2020

Front. Microbiol.

View full text Add to dashboard Cite

Heat stress seriously threatens the growth of Pleurotus ostreatus. Various studies have been performed to study the resistance of P. ostreatus to heat stress. Here, the metabolome was evaluated to determine the response of P. ostreatus mycelia to heat stress at different times (6, 12, 24, 48 h). More than 70 differential metabolites were detected and enriched in their metabolic pathways. Dynamic metabolites changes in enrichment pathways under heat stress showed that heat stress enhanced the degradation of unsaturated fatty acids and nucleotides, increased the content of amino acids and vitamins, and accelerated glycolysis and the tricarboxylic acid cycle in P. ostreatus. The time course changes of P. ostreatus metabolites under continuous heat stress demonstrated that amino acids continuously changed with heat stress, nucleotides clearly changed with heat stress at 12 and 48 h, and lipids exhibited an increasing trend with prolonged heat stress, while few types saccharides and vitamins changed under heat stress. Additionally, heat-treated P. ostreatus produced salicylic acid and other stress-resistant substances that were reported in plants. This study first reported the metabolites changes in P. ostreatus mycelia during 48 h of heat stress. The metabolic pathways and substances that changed with heat stress in this research will aid future studies on the resistance of P. ostreatus and other edible fungi to heat stress.

show abstract

Section: Introductionmentioning

confidence: 99%

Metabolic Response of Pleurotus ostreatus to Continuous Heat Stress

Yan

Zhao

et al. 2020

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Further, the gene expression profiles of various crops have been widely investigated which has revealed many stress responsive genes (Gehan et al , Guo et al 2016a, b, Jacob et al ). Moreover, the role of H 2 S as a regulator of stress tolerance has also been widely studied (Guo et al 2016a, b, Hasanuzzaman et al , Guo et al , Tian et al ). This review is an attempt to summarize the role of H 2 S in cross adaptation in plants along with the regulatory genes involved in H 2 S homeostasis.…”

Section: Introductionmentioning

confidence: 99%

Stress responsive gene regulation in relation to hydrogen sulfide in plants under abiotic stress

Pandey

Gautam

2020

Physiologia Plantarum

View full text Add to dashboard Cite

Plants often face a variety of abiotic stresses, which affects them negatively and lead to yield loss. The antioxidant system efficiently removes excessive reactive oxygen species and maintains redox homeostasis in plants. With better understanding of these protective mechanisms, recently the concept of hydrogen sulfide (H2S) and its role in cell signaling has become the center of attention. H2S has been recognized as a third gasotransmitter and a potent regulator of growth and development processes such as germination, maturation, senescence and defense mechanism in plants. Because of its gaseous nature, H2S can diffuse to different part of the cells and balance the antioxidant pools by supplying sulfur to cells. H2S showed tolerance against a plethora of adverse environmental conditions like drought, salt, high temperature, cold, heavy metals and flood via changing in level of osmolytes, malonaldialdehyde, Na+/K+ uptake, activities of H2S biosynthesis and antioxidative enzymes. It also promotes cross adaptation through persulfidation. H2S along with calcium, methylglyoxal and nitric oxide, and their cross talk induces the expression of mitogen activated protein kinases as well as other genes in response to stress. Therefore, it is sensible to evaluate and explore the stress responsive genes involved in H2S regulated homeostasis and stress tolerance. The current article is aimed to summarize the recent updates on H2S‐mediated gene regulation in special reference to abiotic stress tolerance mechanism, and cross adaptation in plants. Moreover, new insights into the H2S‐associated signal transduction pathway have also been explored.

show abstract

“…Accordingly, the GA content is often used as a standard to measure the overall quality of G. lucidum . Previous research has shown that multiple signal molecules (such as, ROS, NO, Ca 2+ , H 2 S) impact GA production 28 , 36 – 38 . Among them, ROS are reported to play roles that regulate GA production.…”

Section: Discussionmentioning

confidence: 99%

GSNOR regulates ganoderic acid content in Ganoderma lucidum under heat stress through S-nitrosylation of catalase

et al. 2022

Self Cite

View full text Add to dashboard Cite

As a master regulator of the balance between NO signaling and protein S-nitrosylation, S-nitrosoglutathione (GSNO) reductase (GSNOR) is involved in various developmental processes and stress responses. However, the proteins and specific sites that can be S-nitrosylated, especially in microorganisms, and the physiological functions of S-nitrosylated proteins remain unclear. Herein, we show that the ganoderic acid (GA) content in GSNOR-silenced (GSNORi) strains is significantly lower (by 25%) than in wild type (WT) under heat stress (HS). Additionally, silencing GSNOR results in an 80% increase in catalase (CAT) activity, which consequently decreases GA accumulation via inhibition of ROS signaling. The mechanism of GSNOR-mediated control of CAT activity may be via protein S-nitrosylation. In support of this possibility, we show that CAT is S-nitrosylated (as shown via recombinant protein in vitro and via GSNORi strains in vivo). Additionally, Cys (cysteine) 401, Cys642 and Cys653 in CAT are S-nitrosylation sites (assayed via mass spectrometry analysis), and Cys401 may play a pivotal role in CAT activity. These findings indicate a mechanism by which GSNOR responds to stress and regulates secondary metabolite content through protein S-nitrosylation. Our results also define a new S-nitrosylation site and the function of an S-nitrosylated protein regulated by GSNOR in microorganisms.

show abstract

Hydrogen sulfide, a novel small molecule signalling agent, participates in the regulation of ganoderic acids biosynthesis induced by heat stress in Ganoderma lucidum

Cited by 28 publications

References 51 publications

Metabolic Response of Pleurotus ostreatus to Continuous Heat Stress

Metabolic Response of Pleurotus ostreatus to Continuous Heat Stress

Stress responsive gene regulation in relation to hydrogen sulfide in plants under abiotic stress

GSNOR regulates ganoderic acid content in Ganoderma lucidum under heat stress through S-nitrosylation of catalase

Contact Info

Product

Resources

About