Hydrogen-rich water delays postharvest ripening and senescence of kiwifruit

Hu, Huali; Li, Pengxia; Wang, Yuning; Gu, Rongxin

doi:10.1016/j.foodchem.2014.01.067

Cited by 112 publications

(86 citation statements)

References 41 publications

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“…Interestingly, we observed that Arabidopsis plant growth was improved by HRW irrigation (Fig. 1D), further implying that not only the stomatal responses but also some developmental and probably other metabolic pathways are also affected by HRW (Zeng et al, 2013;Hu et al, 2014;Lin et al, 2014).…”

Section: Gork Acts As the Downstream Target Of Hrw Signalingmentioning

confidence: 73%

“…In plants, corresponding results by using HRW combined with gas chromatography (GC) revealed that H 2 could act as a novel beneficial gaseous molecule in plant responses against salinity (Xie et al, 2012;Xu et al, 2013), cadmium stress , and paraquat toxicity (Jin et al, 2013). More recently, the observation that HRW could delay the postharvest ripening and senescence of kiwifruit (Actinidia deliciosa) was reported (Hu et al, 2014).…”

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confidence: 99%

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Reactive Oxygen Species-Dependent Nitric Oxide Production Contributes to Hydrogen-Promoted Stomatal Closure in Arabidopsis

et al. 2014

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The signaling role of hydrogen gas (H 2 ) has attracted increasing attention from animals to plants. However, the physiological significance and molecular mechanism of H 2 in drought tolerance are still largely unexplored. In this article, we report that abscisic acid (ABA) induced stomatal closure in Arabidopsis (Arabidopsis thaliana) by triggering intracellular signaling events involving H 2 , reactive oxygen species (ROS), nitric oxide (NO), and the guard cell outward-rectifying K + channel (GORK). ABA elicited a rapid and sustained H 2 release and production in Arabidopsis. Exogenous hydrogen-rich water (HRW) effectively led to an increase of intracellular H 2 production, a reduction in the stomatal aperture, and enhanced drought tolerance. Subsequent results revealed that HRW stimulated significant inductions of NO and ROS synthesis associated with stomatal closure in the wild type, which were individually abolished in the nitric reductase mutant nitrate reductase1/2 (nia1/2) or the NADPH oxidasedeficient mutant rbohF (for respiratory burst oxidase homolog). Furthermore, we demonstrate that the HRW-promoted NO generation is dependent on ROS production. The rbohF mutant had impaired NO synthesis and stomatal closure in response to HRW, while these changes were rescued by exogenous application of NO. In addition, both HRW and hydrogen peroxide failed to induce NO production or stomatal closure in the nia1/2 mutant, while HRW-promoted ROS accumulation was not impaired. In the GORK-null mutant, stomatal closure induced by ABA, HRW, NO, or hydrogen peroxide was partially suppressed. Together, these results define a main branch of H 2 -regulated stomatal movement involved in the ABA signaling cascade in which RbohF-dependent ROS and nitric reductase-associated NO production, and subsequent GORK activation, were causally involved.

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Section: Gork Acts As the Downstream Target Of Hrw Signalingmentioning

confidence: 73%

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confidence: 99%

Reactive Oxygen Species-Dependent Nitric Oxide Production Contributes to Hydrogen-Promoted Stomatal Closure in Arabidopsis

et al. 2014

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“…Similarly, hydrogen sulphide (H 2 S) improved root elongation inhibition triggered by excess B by targeting cell wall-related PME (Wang et al, 2010). In kiwifruit, H 2 was confirmed to suppress the activity of PME and alleviate pectin solubilization (Hu et al, 2014).…”

Section: Hydrogen Alleviated Boron Toxicity By Modulating Ros Homeostmentioning

confidence: 99%

“…Similar to the approach used in animals, hydrogen-rich water was regarded as a safe and easily available means of investigating the physiological function of endogenous H 2 in plants. It has been shown that H 2 might be a novel bioregulator involved in phytohormone signalling , the delay of fruit senescence (Hu et al, 2014) and plant responses to various stresses, including paraquat (Jin et al, 2013), ultraviolet radiation (Su et al, 2014;Xie et al, 2015), drought (Xie et al, 2014), salinity (Xie et al, 2012;Xu et al, 2013), cadmium and mercury exposure (Cui et al, 2014). However, whether H 2 regulates plant adaptive responses to B toxicity is unknown.…”

Section: Introductionmentioning

confidence: 99%

Linking hydrogen-mediated boron toxicity tolerance with improvement of root elongation, water status and reactive oxygen species balance: a case study for rice

Wang

Duan

et al. 2016

Ann Bot

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Background and aims Boron is essential for plant growth but hazardous when present in excess. As the antioxidant properties of hydrogen gas (H 2 ) were recently described in plants, oxidative stress induced by excess boron was investigated along with other biological responses during rice (Oryza sativa) seed germination to study the beneficial role of H 2 .Methods Rice seeds were pretreated with exogenous H 2 . Using physiological, pharmacological and molecular approaches, the production of endogenous H 2 , growth status, reactive oxygen species (ROS) balance and relative gene expression in rice were measured under boron stress to investigate mechanisms of H 2 -mediated boron toxicity tolerance.Key Results In our test, boron-inhibited seed germination and seedling growth, and endogenous H 2 production, were obviously blocked by exogenously applying H 2 . The re-establishment of ROS balance was confirmed by reduced lipid peroxidation and ROS accumulation. Meanwhile, activities of catalase (CAT) and peroxidase (POX) were increased. Suppression of pectin methylesterase (PME) activity and downregulation of PME transcripts by H 2 were consistent with the alleviation of root growth inhibition caused by boron. Water status was improved as well. This result was confirmed by the upregulation of genes encoding specific aquaporins (AQPs), the maintenance of low osmotic potential and high content of soluble sugar. Increased transcription of representative AQP genes (PIP2;7 in particular) and BOR2 along with decreased BOR1 mRNA may contribute to lowering boron accumulation.Conclusions Hydrogen provides boron toxicity tolerance mainly by improving root elongation, water status and ROS balance.Key words: Oryza sativa, boron toxicity, seed germination, root elongation, hydrogen gas, ROS balance, water status, aquaporins. INTRODUCTIONAlthough boron (B) is an essential micronutrient for plant growth, an excessive concentration of B due to arid and saline soils, as well as low rainfall and poor irrigation, usually produces toxicity in plants, including inhibition of seed germination and seedling growth and reduction of crop yield (e.g. Reid et al., 2004;Roessner et al., 2006;Miwa et al., 2007). The inhibition of root elongation has been found to be one of the most distinct symptoms among all the responses to B toxicity in plants (e.g. Chio et al., 2007;Tanaka and Fujiwara, 2008), and it has been reported that pectin methylesterase (PME) and osmotic potential are involved in this process (Chio et al., 2007;Tanaka and Fujiwara, 2008). Due to the excess B normally occurring in arid and semiarid areas, water stress is another serious problem (e.g. Ben-Gal and Shani, 2003;Reid et al., 2009;Pandey and Archana, 2013). Several genes encoding B transporters have been identified to play roles in B absorption or providing tolerance to B toxicity, including PIP2;4, PIP2;7 (Kumar et al., 2014), TIP5;1 (Pang et al., 2010) and BOR1 (Nakagawa et al., 2007), as well as Bot1 in barley (Sutton et al., 2007) and BOR4 in Arabidopsis . Excess of B coul...

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“…Also, H 2 was suggested to promote adventitious root development. 25,26 Signaling molecules exhibit positive role on adventitious root development…”

Section: 24mentioning

confidence: 99%