Hydrogen-rich saline protects spermatogenesis and hematopoiesis in irradiated BALB/c mice

Chuai, Yunhai; Shen, Jianliang; Qian, Liren; Wang, Yicun; Huang, Yuecheng; Gao, Fu; Cui, Jianguo; Jin, Ning; Zhao, Ling; Liu, Shulin; Sun, Xuejun; Li, Bailong; Cai, Jianming

doi:10.12659/msm.882513

Cited by 30 publications

(17 citation statements)

References 30 publications

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“…Recent basic and clinical research has revealed that hydrogen is an important physiological regulatory factor with antioxidant, anti-inflammatory and antiapoptotic protective effects on cells and organs [5][6][7][8][9][10], proving that hydrogen could down-regulate cytokines, including CCL2, IL-1b, IL-6, IL-12, TNF-a, etc. We also have proposed and proved that hydrogen has radioprotective effects in cultured cells and mice [11][12][13][14][15][16][17]. It also has been demonstrated that H 2 is effective in the prevention of cerebral, myocardial, hepatic ischaemia-reperfusion injuries and other injuries [18,19].…”

Section: Introductionmentioning

confidence: 99%

Hydrogen therapy may be an effective and specific novel treatment for Acute Graft‐versus‐host disease (GVHD)

Qian

Shen

2013

J Cellular Molecular Medi

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Allogeneic haematopoietic stem cell transplantation (HSCT) has been widely used for the treatment of haematological malignant and non-malignant haematologic diseases. However, acute graft-versus-host disease (aGVHD) is a kind of severe complication of HSCT limiting its application. Cytokines such as tumour necrosis factor-α (TNF-α), IL-6 play an extremely important role in the formation and development of aGVHD. Besides, the oxidation phenomena and/or the formation of free radicals have been suggested to be causally related to various haematological disorders including aGVHD. Reactive oxygen species (ROS), such as hydroxyl radicals, play an important role in the formation and development of aGVHD. Hydrogen has been reported to have the ability to inhibit levels of cytokines such as TNF, IL-6 in vivo. Our recent studies provided evidence that hydrogen inhalation can selectively reduce cytotoxic oxygen radicals and exert antioxidant effects. Therefore, we suggested that hydrogen may have therapeutic effects on aGVHD. This hypothesis entails many experimentally testable predictions. We propose the experimental study by detecting complete blood counts (CBC) and Clinic signs of aGVHD mice. We also propose to detect the levels of TNF-α, IL-2, IL-1β, IL-6 which play important roles in the pathogenesis of aGVHD. To discover potential mechanisms of the therapeutic effects of hydrogen on the aGVHD model, we will examine gene-expression profiles. This study will open a new therapeutic avenue combining the field of therapeutic medical gases and aGVHD. This theory is original and probably of importance, because therapeutic medical gases have never been used for aGVHD previously.

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Section: Introductionmentioning

confidence: 99%

Hydrogen therapy may be an effective and specific novel treatment for Acute Graft‐versus‐host disease (GVHD)

Qian

Shen

2013

J Cellular Molecular Medi

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“…Ionizing radiation-induced tissue damage was caused mainly by hydroxyl radicals (11) . Hydroxyl radicals can easily react with cellular macromolecules such as DNA, protein and lipid, to exert strong cytotoxic effects (8,12) . Ohsawa et al (6) found hydrogen could selectively reduce the hydroxyl radical without react with other reactive oxygen species, which possess physiological roles and effective protect cells against oxidative stress damage.…”

Section: Discussionmentioning

confidence: 99%

Radio-protective effect of hydrogen rich water combined with amifostine in mice

Qin

Yin

et al. 2016

IJRR

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Background: Hydrogen has been demonstrated can selec vely reduce the hydroxyl, which is the main cause of ionizing radia on-induced damage. Amifos ne (AM) is the only radioprotec ve drug approved by the U.S. Food and Drug Administra on for use in radiotherapy. The purpose of the present study was to inves gate the combined radio-protec ve effect of hydrogen rich water (HRW) and AM. Materials and Methods: Male ICR mice were treated intragastrically with HRW or/and intraperitoneally with AM 30 minutes prior to 9.0 Gy whole body irradia on from a 60 Co source (dose rate 0.96Gy/min). Then the survival rate for 30 days, the hematological parameters, the Clinical chemistry parameters and the bone marrow nucleated cells were examined. Results: We found that the mice treated with HRW and AM before irradia on could increase the 30-day survival rate and improve the body weight be2er than the HRW or AM treatment alone group and irradia on alone group. Hematological test and Clinical chemistry assays also showed the same results that HRW combined AM could be2er improve the recovery of hemopoie c system and alleviate the detrimental effects of radia on. Conclusion: The results indicate that the combined applica on of HRW and AM may be a be2er method for radia on therapy.

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“…As we previously reported [18][19][20], hydrogen was dissolved in physiological saline 6 hrs under high pressure (0.4 MPa) to a supersaturated level using hydrogen-rich water producing apparatus produced by our department. The saturated hydrogen saline was stored under Liren Qian and Xiaopeng Liu contribute equally to the paper.…”

Section: Hydrogen-rich Saline Productionmentioning

confidence: 99%

Therapeutic effects of hydrogen on chronic graft‐versus‐host disease

Qian

Liu

Shen

et al. 2017

J Cellular Molecular Medi

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The incidence of chronic graft‐versus‐host disease (cGVHD) is rising recent years, which has been the leading cause of non‐transplantation mortality post allogenetic hematopoietic stem cell transplantation (HSCT). Imbalance of inflammatory cytokines and fibrosis plays critical roles in the pathogenesis of cGVHD. Recent studies showed that molecular hydrogen has anti‐inflammatory, antioxidant, anti‐fibrosis effects. Therefore, we hypothesized that molecular hydrogen may have therapeutic effects on cGVHD. To determine whether hydrogen could protect mice from cGVHD in an MHC‐incompatible murine bone marrow transplantation (BMT) model, survival rates of mice were calculated, and skin lesions were also evaluated after BMT. This article demonstrated that administration of hydrogen‐rich saline increased survival rate of cGVHD mice. Administration of hydrogen‐rich saline after transplantation also reduced skin lesions of cGVHD mice. Previously, we reported the therapeutic effects of hydrogen on acute GVHD. However, there was no report on the therapeutic effects of hydrogen on cGVHD mice. It is suggested that hydrogen has a potential as an effective and safe therapeutic agent on cGVHD. This study will provide new ideas on the treatment of cGVHD and has important theoretical values.

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Hydrogen-rich saline protects spermatogenesis and hematopoiesis in irradiated BALB/c mice

Cited by 30 publications

References 30 publications

Hydrogen therapy may be an effective and specific novel treatment for Acute Graft‐versus‐host disease (GVHD)

Hydrogen therapy may be an effective and specific novel treatment for Acute Graft‐versus‐host disease (GVHD)

Radio-protective effect of hydrogen rich water combined with amifostine in mice

Therapeutic effects of hydrogen on chronic graft‐versus‐host disease

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