Hydrogen-rich saline protects immunocytes from radiation-induced apoptosis

Yang, Yue; Li, Bailong; Liu, Cong; Chuai, Yunhai; Lei, Jixiao; Fu, Guosheng; Cui, Jianguo; Sun, Ding; Cheng, Ying; Zhou, Chunyun; Cai, Jianming

doi:10.12659/msm.882616

Cited by 36 publications

(33 citation statements)

References 21 publications

(21 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mice received total-body radiation at the radiation center in the faculty of Naval Medicine, Second Military Medical University, China [19, 20], and the survival of mice was recorded.…”

Section: Methodsmentioning

confidence: 99%

Polymyxin B Attenuates LPS-Induced Death but Aggravates Radiation-Induced Death via TLR4-Myd88-IL-6 Pathway

Cheng¹,

Du²,

Han³

et al. 2017

Cell Physiol Biochem

Self Cite

View full text Add to dashboard Cite

Background/Aims: Polymyxin B (PMB) is a cyclic cationic polypeptide antibiotic widely used to counteract the effects of endotoxin contamination, both in vitro and in vivo. Lipopolysaccharide (LPS) is an endotoxin that acts as a radiation protection factor. In this study, we focus on the role of PMB in LPS-induced and radiation-induced mortality in mice. Methods: Mice received total-body radiation or were pretreated by LPS or PMB, and the survival of mice was recorded. Elisa were used to detect the cytokines levels. Results: PMB decreased LPS-induced, but increased radiation-induced mortality in mice. Moreover, PMB could block the LPS-induced radioprotective effect. The ELISA and gene knock-out experiments indicated that PMB reduces TNF-α level to block LPS-induced mortality in mice, and inhibits IL-6, G-CSF and IL-10 to increase radiation-induced mortality via the TLR4-Myd88-IL-6 pathway. Conclusions: Our study revealed a role of PMB in LPS-induced endotoxemia and radiation exposure. We infer that the TLR4-Myd88-IL-6 pathway may play a crucial role in the process.

show abstract

“…Mice received total-body radiation at the radiation center in the faculty of Naval Medicine, Second Military Medical University, China [19, 20], and the survival of mice was recorded.…”

Section: Methodsmentioning

confidence: 99%

Polymyxin B Attenuates LPS-Induced Death but Aggravates Radiation-Induced Death via TLR4-Myd88-IL-6 Pathway

Cheng¹,

Du²,

Han³

et al. 2017

Cell Physiol Biochem

Self Cite

View full text Add to dashboard Cite

show abstract

“…IR causes bone marrow suppression, which suppress the hematopoietic stem and progenitor cells proliferation and differentiation (Wang et al, 2006;Meng et al, 2003). The rapidly dividing cells of the blood system, especially leukocytes and erythrocytes, and the immune organs and immune cells are highly sensitive to IR (Widel et al, 2003;Yang et al, 2012). Radiation significantly decreased peripheral blood WBC, platelet and RBC counts (Jiang et al, 2015;Suryavanshi et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Peer Review #3 of "Pine polyphenols from Pinus koraiensis prevent injuries induced by gamma radiation in mice (v0.1)"

Jurin¹

2016

View full text Add to dashboard Cite

Pine polyphenols (PPs) are bioactive dietary constituents, enhance health and help prevent diseases through antioxidant. Antioxidants reduce the level of oxidative damages caused by ionizing radiation (IR). The main purpose of this paper is to study the protective effect of PPs on peripheral blood, liver and spleen injuries in mice induced by IR. ICR (Institute of Cancer Research) male mice were administered orally with PPs (200 mg/kg b.wt.) once daily for 14 consecutive days prior to 7 Gy γ-radiations. PPs showed strong antioxidant activities. PPs significantly increased white blood cells, red blood cells and platelets counts. PPs also significantly reduced lipid peroxidation and increased the activities of superoxide dismutase, catalase and glutathione peroxidases, and the level of glutathione. PPs reduced the spleen morphologic injury. In addition, PPs inhibited mitochondria-dependent apoptosis pathways in splenocytes induced by IR. These results indicate that PPs are radioprotective promising reagent.

show abstract

“…However, a large portion of patients may fail in responding to the treatments or relapse after the treatment [22]. Recently, we found that hydrogen-rich solution can protect the immune system and accelerate the recovery of immunodeficiency induced by irradiation [23]. Meanwhile, our research determined that hydrogen can protect mice from GVHD by regulating TNF-α, IL-2, IL-10 [17].…”

Section: Discussionmentioning

confidence: 99%

Therapeutic Effects of Hydrogen-Rich Solution on Aplastic Anemia in Vivo

Zhao

Mei

Qian

et al. 2013

Cell Physiol Biochem

Self Cite

View full text Add to dashboard Cite

Background: Aplasitc anemia (AA) is a bone marrow failure syndrome characterized by an immune-mediated destruction of hematopoietic stem cells. Though clinical symptoms could be ameliorated by bone marrow transplantation and/or immunosuppressive therapy, frequent recurrence and especially evolution of clonal hematologic diseases remains problematic clinically. Cytokines such as interferon-γ (INF-γ), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) secreted by autologous T cells are closely related with the development of AA. Hydrogen-rich solution was reported to inhibit the levels of cytokines including INF-γ, TNF-α and IL-6 in vivo in recent studies. This study was to investigate the potential therapeutic effects of hydrogen-rich solution on AA in vivo. Methods: AA model was determined in vivo by mice and body weights of the mice were used as the basic physiological index. Peripheral blood cells were calculated to evaluate the hematologic recovery degree. Bone marrow nucleated cells (BMNCs), tissue histology, as well as CFU-S and CFU-GM forming units were used to evaluate the recovery of bone marrow microenvironment. The ratio of CD4⁺ and CD8⁺ cells were examined along with cytokine levels in serum to determine the efficacy of H₂-rich solution on the affected immunological functions. Results: Body weight and number of peripheral blood cells were significantly improved for mice in the H₂-rich solution treated groups as compared with those with AA. The number of BMNCs and CFUs increased markedly and the bone marrow microenvironment was also improved significantly. The experimental group restrained the cell apoptosis, relieved hyperemia and accelerated tissue repair. The number of CD4⁺ and CD8⁺ cells as well as the ratio of CD4/CD8 increased to normal gradually, while the levels of TNF-α, IFN-γ, and IL-6 in serum decreased after H₂-rich solution treatment. Conclusion: Our study firstly showed that hydrogen-rich solution accelerated the recovery of either hematological or immunological recovery on aplastic anemia mice. This finding suggests hydrogen-rich solution as a potential clinical therapeutic agent for AA.

show abstract

Hydrogen-rich saline protects immunocytes from radiation-induced apoptosis

Cited by 36 publications

References 21 publications

Polymyxin B Attenuates LPS-Induced Death but Aggravates Radiation-Induced Death via TLR4-Myd88-IL-6 Pathway

Polymyxin B Attenuates LPS-Induced Death but Aggravates Radiation-Induced Death via TLR4-Myd88-IL-6 Pathway

Peer Review #3 of "Pine polyphenols from Pinus koraiensis prevent injuries induced by gamma radiation in mice (v0.1)"

Therapeutic Effects of Hydrogen-Rich Solution on Aplastic Anemia in Vivo

Contact Info

Product

Resources

About