Hydrogen-rich saline injection into the subarachnoid cavity within 2 weeks promotes recovery after acute spinal cord injury

Wang, Jianlong; Zhang, Qingshan; Zhu, Kai-di; Sun, Jianfeng; Zhang, Ze-peng; Sun, Jian-Wen; Zhang, Kexiang

doi:10.4103/1673-5374.158361

Cited by 14 publications

(11 citation statements)

References 45 publications

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“…Subarachnoid cavity injection of HRS improved motor function in rat hindlimb, decreased cell death, inflammatory reaction and oxidative damage in a taumatic spinal cord injury model. 17 HRS also significantly decreased the levels of lipid peroxide, vascular endothelial growth factor, thrombomodulin, and increased the glutathione level and microvessel density in a rabbit model of steroid-associated necrosis of the femoral head, which indicated that hydrogen might provide an alternative treatment for steroid-associated necrosis of the femoral head. 67 Researchers also investigated the beneficial effect of hydrogen in a duchenne muscular dystrophy animal model, and found hydrogen increased the spontaneous running distance and the weight of the offsprings, decreased plasma creatine kinase activities and the number of central nuclei of muscle fibers at ages 10 and 24 weeks.…”

Section: T He R Esearch P mentioning

confidence: 84%

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The transfer of hydrogen from inert gas to therapeutic gas

Shen

et al. 2017

Med Gas Res

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Hydrogen is the most abundant chemical element in the universe, and has been used as an inert gas for a long time. More recent studies have shown that molecular hydrogen as a kind of antioxidant, anti-inflammatory, anti-apoptosis, gene expression and signal modulation molecule, can be used for the treatment of many diseases. This review mainly focuses on the research progresses of hydrogen in various medical fields and the possible action mechanisms.

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Section: T He R Esearch P mentioning

confidence: 84%

“… 16 Subarachnoid cavity injection of hydrogen rich saline (HRS) has also become a new treatment for brain and spinal cord diseases. 17 …”

Section: T He I Njection M mentioning

confidence: 99%

The transfer of hydrogen from inert gas to therapeutic gas

Shen

et al. 2017

Med Gas Res

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“…Therefore, oxidative events that occur during the pathogenesis of secondary injury after SCI leading to apoptosis is a major complicator of this process (32). Various studies have shown that irrespective of whether it is ischemia/reperfusion or trauma-related injuries on neural tissue or multiple organs, apoptosis has vigorously increased (32)(33)(34)(35). In our previous studies, we have shown an increase in caspase-3 activity and protein expression in SCI-in-duced spinal cord, bladder, and corpus cavernosum injuries (21,27,36).…”

Section: Discussionmentioning

confidence: 99%

Riboflavin Treatment Reduces Apoptosis and Oxidative DNA Damage in a Rat Spinal Cord Injury Model

Sakarcan¹,

Erşahin²,

Eminoğlu³

et al. 2017

Clin Exp Health Sci

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Amaç: Omurilik hasarı inflamatuar yanıta ve oksidatif strese yol açarak çeşitli organ sistemlerinde zararlı etkiler oluşturur. Riboflavin insan ve hayvanda sağlığın sürdürülmesinde önemli role sahip olan ve kolayca absorbe edilen mikrobesindir. Bu çalışma omurilik yaralanmasına bağlı omurilik ve böbrek dokusunda riboflavinin koruyucu etkilerini araştırmak üzere planlandı. Yöntemler: Omurilik hasarı oluşturmak için anestezi altındaki sıçanlara T10 seviyesinde 100 g/cm şiddetinde ağırlık düşürme metodu uygulandı. Hasarlı hayvanlara riboflavin 25 mg/kg dozunda ya da taşıyıcı çözelti tedavisi hasardan 15 dakika sonra verildi ve 7 gün süreyle devam edildi. Hasardan sonra 7.günde nörolojik testin arkasından hayvanlar dekapite edilerek spinal ve böbrek dokuları alındı. Dokularda histolojik tayinler yapıldı ve malondialdehit (MDA), glutatyon (GSH), 8-hidroksi-2-deoksiguanozin (8-OHdG) düzeyleri ile myeloperoksidaz (MPO), süperoksid dismutaz (SOD) ve katalaz aktiviteleri tayin edildi. Bulgular: Omurilik hasarı dokularda GSH düzeylerinde ve SOD aktivitesinde azalamaya, MDA düzeyinde ve MPO ve kaspaz aktivitelerinde artışa neden oldu. Riboflavin tedavisi bu parametreleri geri çevirdi ve histolojik bulgularda düzelme gösterdi. Sonuç: Çalışmamızda Omurilik hasarı, dokuya nötrofil göçüne ve oksidan strese yol açarken, antiapoptotik ve nöroprotektif özellikleri ile riboflavin lipid peroksidasyonunu ve nötrofillerin dokuya infiltrasyonunu inhibe etti. Ayrıca, çalışmamız riboflavinin antiapoptotik ve antioksidan etkisinin sadece omurilikte değil omurilik hasarında ikincil olarak ortaya çıkan böbrek hasarında da önemli faydaları olduğunu gösterdi.

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“…HRS, a convenient and safe way to provide H 2 , has been reported in a large number of studies. 1 31 32 H 2 can easily pass through biological tissue barriers, such as blood-brain barrier and blood-eye barrier, to reach target organs. It has been proved to reduce oxidative stress in various organs and tissues, such as the brain, eyes, lungs, liver, kidney.…”

Section: Discussionmentioning

confidence: 99%

Effects of hydrogen-rich saline on endotoxin-induced uveitis

et al. 2017

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The therapeutic effects of hydrogen-rich saline (HRS) have been reported for a wide range of diseases mainly via selectively reducing the amount of reactive oxygen species. Oxidative stress plays an important role in the pathogenesis of uveitis and endotoxin-induced uveitis (EIU). In this study, we investigated whether HRS can mitigate EIU in rats. Sprague-Dawley rats were randomly divided into Norm group, Model group, HRS group, dexamethasone (DEX) group, and rats in the latter three groups were injected with equal amount of lipopolysaccharide (LPS) to induce EIU of different severities (by 1 mg/kg of LPS, or 1/8 mg/kg of LPS). Rats in HRS group were injected with HRS intraperitoneally at three different modes to purse an ameliorating effect of EIU (10 mL/kg of HRS immediately after injection of 1 mg/kg of LPS, 20 mL/kg of HRS once a day for 1 week before injection of 1 mg/kg of LPS and at 0, 0.5, 1, 2, 6, 8, 12 hours after LPS administration, or 20 mL/kg of HRS once a day for 1 week before injection of 1/8 mg/kg of LPS, and at 0, 0.5, 1, 2, 6, 8, 12, 24 hours and once a day for 3 weeks after LPS administration). Rats of DEX group were injected with 1 mL/kg of DEX solution intraperitoneally immediately after LPS administration. Rats in Norm and Model groups did not receive any treatment. All rats were examined under slit lamp microscope and graded according to the clinical signs of uveitis. Electroretinogram, quantitative analysis of protein in aqueous humor (AqH) and histological examination of iris and ciliary body were also carried out. Our results showed that HRS did not obviously ameliorate the signs of uveitis under slit lamp examination and the inflammatory cells infiltration around iris and cilliary body of EIU induced by 1 mg/kg or 1/8 mg/kg of LPS (P > 0.05), while DEX significantly reduced the inflammation reflected by the above two indicators (P < 0.05). The impaired retinal function of mild EIU induced by 1/8 mg/kg of LPS, showed by delay of peak time of b-wave of Dark adapted 3.0 electroretinogram, was not significantly restored by HRS (P > 0.05), while DEX had an obvious therapeutic effect (P < 0.05). However, HRS exerted an inhibition trend on elevation of protein in AqH of EIU induced by 1 mg/kg of LPS, and significantly reduced the increasing amount of protein in AqH of mild EIU induced by 1/8 mg/kg of LPS (P < 0.05). In conclusion, HRS could not obviously mitigate EIU in rats, while it could inhibit the elevation of AqH protein.

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Hydrogen-rich saline injection into the subarachnoid cavity within 2 weeks promotes recovery after acute spinal cord injury

Cited by 14 publications

References 45 publications

The transfer of hydrogen from inert gas to therapeutic gas

The transfer of hydrogen from inert gas to therapeutic gas

Riboflavin Treatment Reduces Apoptosis and Oxidative DNA Damage in a Rat Spinal Cord Injury Model

Effects of hydrogen-rich saline on endotoxin-induced uveitis

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